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Susan Fanayan Mona Shehata Annelies P Agterof Michael A McGuckin Miguel A Alonso Jennifer A Byrne 《BMC cell biology》2009,10(1):7-13
Background
The MAL2 gene, encoding a four-transmembrane protein of the MAL family, is amplified and overexpressed in breast and other cancers, yet the significance of this is unknown. MAL-like proteins have trafficking functions, but their molecular roles are largely obscure, partly due to a lack of known binding partners. 相似文献954.
Molecular phylogeny of ocelloid-bearing dinoflagellates (Warnowiaceae) as inferred from SSU and LSU rDNA sequences 总被引:1,自引:0,他引:1
Mona Hoppenrath Tsvetan R Bachvaroff Sara M Handy Charles F Delwiche Brian S Leander 《BMC evolutionary biology》2009,9(1):116-15
Background
Dinoflagellates represent a major lineage of unicellular eukaryotes with unparalleled diversity and complexity in morphological features. The monophyly of dinoflagellates has been convincingly demonstrated, but the interrelationships among dinoflagellate lineages still remain largely unresolved. Warnowiid dinoflagellates are among the most remarkable eukaryotes known because of their possession of highly elaborate ultrastructural systems: pistons, nematocysts, and ocelloids. Complex organelles like these are evolutionary innovations found only in a few athecate dinoflagellates. Moreover, the taxonomy of warnowiids is extremely confusing and inferences about the evolutionary history of this lineage are mired by the absence of molecular phylogenetic data from any member of the group. In this study, we provide the first molecular phylogenetic data for warnowiids and couple them with a review of warnowiid morphological features in order to formulate a hypothetical framework for understanding character evolution within the group. These data also enabled us to evaluate the evolutionary relationship(s) between warnowiids and the other group of dinoflagellates with complex organelles: polykrikoids. 相似文献955.
Mona Utne Larsen Matthew Seward Anubhav Tripathi Nina C. Shapley 《Biotechnology progress》2009,25(4):1094-1102
This study reveals an exciting phenomenon of stimulated bacteria clustering. Rapid aggregation and microbial arrest are shown to occur in Escherichia coli solutions of neutral pH when chitosan nanoparticles with positive zeta potential are added. Because chitosan nanoparticles can easily be dispersed in aqueous buffers, the rapid clustering phenomenon requires only minuscule nanoparticle concentrations and will be critical in developing new methods for extricating bacterial pathogens. This work establishes the dominant role of electrostatic attraction in bacteria‐nanoparticle interactions by varying the nanoparticle zeta potential from highly positive to strongly negative values, and by exploring concentration effects. For strongly negative nanoparticles, no clusters form, while aggregates are small and loose at intermediate conditions. In addition, optical density measurements indicate that over 90% of the suspended bacteria flocculate within seconds of being mixed with chitosan nanoparticles of a highly positive surface charge. Finally, the nanoparticles are significantly more efficient as a clustering agent compared to an equal mass of molecular chitosan in solution, as the bacteria‐nanoparticle clusters formed are substantially larger. The bacteria‐nanoparticle aggregation effect demonstrated here promises a rapid separation method for aiding pathogen detection and for flocculation of bacteria in fermentation processes. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 相似文献
956.
Anderson GJ Bernardello G Opel MR Santos-Guerra A Anderson M 《American journal of botany》2006,93(9):1295-1305
We confirmed functional dioecy of Withania aristata via field and greenhouse studies. Male flowers are significantly larger. Female flowers bear stamens with no pollen; males bear 220?000 grains. Stigmata of male flowers senesce in buds. Anatomical observations confirm more ovules in females and an ovarian nectary in both sexes. We detected nectar in female flowers in the greenhouse but found no nectar in males. Thus, males offer pollen and females nectar. Females bear large numbers of fruits and, infrequently, male plants bear few significantly smaller fruits with few seeds. Outcrosses of females (self crosses impossible without pollen) yielded fruits in young buds, older buds, and open flowers. Self crosses of male flowers succeeded only with very young buds. Although functionally dioecious, this species manifests self-compatibility; however, no fruits are produced autonomously. Bee species (Lassioglossum, Amegilla, Apis) visit flowers and mature buds. Bud visits in which bees force petal tips apart, coupled with self-compatibility, may explain infrequent fruit on males. Thus, dioecy in W. aristata seems to have evolved from self-compatible ancestors, that leaky dioecy may have been favored during colonization, and, that despite autogamy and a low floral visition rate, this endemic enjoys a high rate of reproductive success. 相似文献
957.
The low plant productivity of boreal forests in general has been attributed to low soil N supply and low temperatures. Exceptionally
high productivity occurs in toe-slope positions, and has been ascribed to influx of N from surrounding areas and higher rates
of soil N turnover in situ. Despite large apparent natural variations in forest productivity, rates of gross soil N mineralization
and gross nitrification have never been compared in Fennoscandian boreal forests of contrasting productivity. We report contrasting
patterns of soil N turnover in three model ecosystems, representing the range in soil C-to-N ratios (19–41) in Fennoscandian
boreal forests and differences in forest productivity by a factor close to 3. Gross N mineralization was seven times higher
when soil, microbial, and plant C-to-N ratios were the lowest compared to the highest. This process, nitrification and potential
denitrification correlated with inorganic, total and microbial biomass N, but not microbial C. There was a constant ratio
between soil and microbial C-to-N ratio of 3.7±0.2, across wide ratios of soil C-to-N and fungi-to-bacteria. Soil N-cycling
should be controlled by the supplies of C and N to the microbes. In accordance with plant allocation theory, we discuss the
possibility that the high fungal biomass at high soil C-to-N ratio reflects a particularly high supply of plant photosynthates,
substrates of high-quality C, to mycorrhizal fungi. Methods to study soil N turnover and N retention should be developed to
take into account the impact of mycorrhizal fungi on soil N-cycling. 相似文献
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In electron tomography the reconstructed density function is typically corrupted by noise and artifacts. Under those conditions, separating the meaningful regions of the reconstructed density function is not trivial. Despite development efforts that specifically target electron tomography manual segmentation continues to be the preferred method. Based on previous good experiences using a segmentation based on fuzzy logic principles (fuzzy segmentation) where the reconstructed density functions also have low signal-to-noise ratio, we applied it to electron tomographic reconstructions. We demonstrate the usefulness of the fuzzy segmentation algorithm evaluating it within the limits of segmenting electron tomograms of selectively stained, plastic embedded spiny dendrites. The results produced by the fuzzy segmentation algorithm within the framework presented are encouraging. 相似文献