全文获取类型
收费全文 | 1158篇 |
免费 | 66篇 |
国内免费 | 1篇 |
出版年
2024年 | 3篇 |
2023年 | 13篇 |
2022年 | 36篇 |
2021年 | 59篇 |
2020年 | 31篇 |
2019年 | 42篇 |
2018年 | 49篇 |
2017年 | 30篇 |
2016年 | 42篇 |
2015年 | 62篇 |
2014年 | 61篇 |
2013年 | 98篇 |
2012年 | 89篇 |
2011年 | 96篇 |
2010年 | 53篇 |
2009年 | 39篇 |
2008年 | 43篇 |
2007年 | 52篇 |
2006年 | 50篇 |
2005年 | 48篇 |
2004年 | 53篇 |
2003年 | 38篇 |
2002年 | 38篇 |
2001年 | 9篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 8篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1968年 | 3篇 |
1928年 | 2篇 |
1911年 | 1篇 |
排序方式: 共有1225条查询结果,搜索用时 937 毫秒
101.
Xiang MA Rybczynski PJ Patel M Chen RH McComsey DF Zhang HC Gunnet JW Look R Wang Y Minor LK Zhong HM Villani FJ Demarest KT Damiano BP Maryanoff BE 《Bioorganic & medicinal chemistry letters》2007,17(23):6623-6628
We have continued to explore spirobenzazepines as vasopressin receptor antagonists to follow up on RWJ-339489 (2), which had advanced into preclinical development. Further structural modifications were pursued to find a suitable backup compound for human clinical studies. Thus, we identified carboxylic acid derivative 3 (RWJ-676070; JNJ-17158063) as a potent, balanced vasopressin V(1a)/V(2) receptor antagonist with favorable properties for clinical development. Compound 3 is currently undergoing human clinical investigation. 相似文献
102.
Mona Hoppenrath Malte Elbrächter Hannelore Halliger Reinoud P. T. Koeman Alexander Krakhmalnyy Barbara Surek Katrin Erler Bernd Luckas 《Helgoland Marine Research》2007,61(3):157-165
Thecadinium yashimaense was recorded for the first time in France, Great Britain, The Netherlands, and Germany. The invasion and establishment of
the species in the German Bight was documented reliably and is presented here. The geographic expansion of the species from
the North Pacific to the North Atlantic Ocean is discussed. This bloom-forming, marine, sand-dwelling dinoflagellate was shown
to be non-toxic. Also Thecadinium kofoidii, the type species of the genus, was analyzed for potential toxin production and turned out to be non-toxic as well. 相似文献
103.
Hassan Mona M. Taha Rania A. Abd El-Aziz M. E. Shaaban Esam A. Ibrahim Eman A. 《Plant Cell, Tissue and Organ Culture》2022,148(1):73-80
Plant Cell, Tissue and Organ Culture (PCTOC) - Leaf chlorosis is often a problem in micropropagated Rubus idaeus, which makes successful acclimatization difficult. We found that... 相似文献
104.
Dawood Mona F. A. Zaid Abbu Latef Arafat Abdel Hamed Abdel 《Journal of Plant Growth Regulation》2022,41(5):1919-1942
Journal of Plant Growth Regulation - Under the present era of changing climate, plants face simultaneous abiotic pressures rather than single stress. Under these unprecedented and joint... 相似文献
105.
Yu Zhao Mona C Majid Jennifer M Soll Joshua R Brickner Sebastian Dango Nima Mosammaparast 《The EMBO journal》2015,34(12):1687-1703
Repair of DNA alkylation damage is critical for genomic stability and involves multiple conserved enzymatic pathways. Alkylation damage resistance, which is critical in cancer chemotherapy, depends on the overexpression of alkylation repair proteins. However, the mechanisms responsible for this upregulation are unknown. Here, we show that an OTU domain deubiquitinase, OTUD4, is a positive regulator of ALKBH2 and ALKBH3, two DNA demethylases critical for alkylation repair. Remarkably, we find that OTUD4 catalytic activity is completely dispensable for this function. Rather, OTUD4 is a scaffold for USP7 and USP9X, two deubiquitinases that act directly on the AlkB proteins. Moreover, we show that loss of OTUD4, USP7, or USP9X in tumor cells makes them significantly more sensitive to alkylating agents. Taken together, this work reveals a novel, noncanonical mechanism by which an OTU family deubiquitinase regulates its substrates, and provides multiple new targets for alkylation chemotherapy sensitization of tumors. 相似文献
106.
107.
Anna Amcheslavsky Mona?L. Wood Andriy?V. Yeromin Ian Parker J.?Alfredo Freites Douglas?J. Tobias Michael?D. Cahalan 《Biophysical journal》2015,108(2):237-246
Upon endoplasmic reticulum Ca2+ store depletion, Orai channels in the plasma membrane are activated directly by endoplasmic reticulum-resident STIM proteins to generate the Ca2+-selective, Ca2+ release-activated Ca2+ (CRAC) current. After the molecular identification of Orai, a plethora of functional and biochemical studies sought to compare Orai homologs, determine their stoichiometry, identify structural domains responsible for the biophysical fingerprint of the CRAC current, identify the physiological functions, and investigate Orai homologs as potential therapeutic targets. Subsequently, the solved crystal structure of Drosophila Orai (dOrai) substantiated many findings from structure-function studies, but also revealed an unexpected hexameric structure. In this review, we explore Orai channels as elucidated by functional and biochemical studies, analyze the dOrai crystal structure and its implications for Orai channel function, and present newly available information from molecular dynamics simulations that shed light on Orai channel gating and permeation. 相似文献
108.
Kirankumar Katta Tabasum Imran Marta Busse-Wicher Mona Gr?nning Szymon Czajkowski Marion Kusche-Gullberg 《The Journal of biological chemistry》2015,290(21):13168-13177
Heparan sulfate proteoglycans are ubiquitously located on cell surfaces and in the extracellular matrices. The negatively charged heparan sulfate chains interact with a multitude of different proteins, thereby influencing a variety of cellular and developmental processes, for example cell adhesion, migration, tissue morphogenesis, and differentiation. The human exostosin (EXT) family of genes contains five members: the heparan sulfate polymerizing enzymes, EXT1 and EXT2, and three EXT-like genes, EXTL1, EXTL2, and EXTL3. EXTL2 has been ascribed activities related to the initiation and termination of heparan sulfate chains. Here we further investigated the role of EXTL2 in heparan sulfate chain elongation by gene silencing and overexpression strategies. We found that siRNA-mediated knockdown of EXTL2 in human embryonic kidney 293 cells resulted in increased chain length, whereas overexpression of EXTL2 in the same cell line had little or no effect on heparan sulfate chain length. To study in more detail the role of EXTL2 in heparan sulfate chain elongation, we tested the ability of the overexpressed protein to catalyze the in vitro incorporation of N-acetylglucosamine and N-acetylgalactosamine to oligosaccharide acceptors resembling unmodified heparan sulfate and chondroitin sulfate precursor molecules. Analysis of the generated products revealed that recombinant EXTL2 showed weak ability to transfer N-acetylgalactosamine to heparan sulfate precursor molecules but also, that EXTL2 exhibited much stronger in vitro N-acetylglucosamine-transferase activity related to elongation of heparan sulfate chains. 相似文献
109.
Mohammad-Shafie?Rahmani Paula?M.?PijutEmail author Naghi?Shabanian Mona?Nasri 《In vitro cellular & developmental biology. Plant》2015,51(4):407-419
A protocol was established for callus induction and plant regeneration of Albizia julibrissin Durazz., a multipurpose tree. Calli were induced on hypocotyl explants excised from 10- to 14-d-old in vitro seedlings cultured on Murashige and Skoog (MS) medium supplemented with α-naphthaleneacetic acid (NAA) alone or in combination with 6-benzylaminopurine (BA) or 6-furfurylaminopurine (kinetin). The highest frequency of organogenic callus (82.2?±?3.6%) was obtained on MS medium with 10.8 μM NAA and 4.4 μM BA. Calli were then cultured on MS medium with BA or zeatin, singly or in combination, for shoot regeneration. Calli cultured on MS medium with 13.2 μM BA and 4.6 μM zeatin produced the highest frequency of adventitious shoot regeneration (75.3?±?6.3%). Maximum rooting of shoots (73.3?±?5%) was achieved using half-strength MS medium with 4.9 μM indole-3-butyric acid. The genetic fidelity of 12 plants acclimatized to the greenhouse was assessed based on analyses of start codon targeted (SCoT) polymorphism and inter-retrotransposon amplified polymorphism (IRAP). The 14 SCoT and 7 IRAP adapted primers produced 71 and 34 scoreable fragments, of which 33 (46%) and 12 (35%) were polymorphic, respectively. The in vitro-raised plants exhibited 0.129–0.438 genetic distance from the mother plant and 0.000–0.788 distance from one another according to the SCoT and IRAP analyses. Although the culture method described here may not be suitable for clonal propagation of elite genotypes, it can be used for conservation of this plant. 相似文献
110.
Glucagon signalling in the dorsal vagal complex is sufficient and necessary for high‐protein feeding to regulate glucose homeostasis in vivo
下载免费PDF全文
![点击此处可从《EMBO reports》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mary P LaPierre Mona A Abraham Jessica TY Yue Beatrice M Filippi Tony KT Lam 《EMBO reports》2015,16(10):1299-1307
High‐protein feeding acutely lowers postprandial glucose concentration compared to low‐protein feeding, despite a dichotomous rise of circulating glucagon levels. The physiological role of this glucagon rise has been largely overlooked. We here first report that glucagon signalling in the dorsal vagal complex (DVC) of the brain is sufficient to lower glucose production by activating a Gcgr–PKA–ERK–KATP channel signalling cascade in the DVC of rats in vivo. We further demonstrate that direct blockade of DVC Gcgr signalling negates the acute ability of high‐ vs. low‐protein feeding to reduce plasma glucose concentration, indicating that the elevated circulating glucagon during high‐protein feeding acts in the brain to lower plasma glucose levels. These data revise the physiological role of glucagon and argue that brain glucagon signalling contributes to glucose homeostasis during dietary protein intake. 相似文献