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51.
52.
R J Berry R H Mole D W Barnes 《International journal of radiation biology and related studies in physics, chemistry, and medicine》1976,30(6):535-541
Skin reaction to X-irradiation has been studied in the albino guinea-pig; early response in limited-field irradiations of the flank is comparable to that commonly seen in rodents, swine and man, and is dose-dependent with a dynamic range from mild erythema to moist desquamation. The peak early skin reaction is seen between 14 and 21 days after irradiation, and declines before 30 days except at the highest doses used. Fractionation of the X-ray dose at 24 hours results in a 'sparing' of about 340 rad. Permanent partial epilation is detectable at doses in excess of 1400 rad, and complete epilation at 1 year occurs in 50 per cent of irradiated fields at 1740 rad. Twenty-four hour two-dose fractionation results in a 'sparing' of about 500 rad for epilation. Palpable dermal 'fibrosis' is detectable at 3 months after irradiation in fields given more than 2070 rad, and at 1 year after irradiation in fields given more than 1800 rad; 50 per cent of fields showed palpable 'fibrosis' at 1 year at 1930 rad. Unlike domestic swine and man, skin fields in the guinea-pig showed no dimensional contraction after X-ray doses which produced gross early skin damage. 相似文献
53.
A high-performance liquid chromatographic method has been modified for the evaluation of both Phase I and II metabolism of biphenyl by hepatocytes maintained in an embryo/hepatocyte co-culture medium. Extracts of the media, before and after hydrolysis of conjugates, are directly injected onto the HPLC and the major hydroxylated metabolites plus unmetabolized biphenyl are detected by fluorescence after separation under gradient or isocratic conditions. The method is almost free of interferences and is relatively simple and rapid. In the case of the monohydroxylated derivatives, the minimum media concentrations which can be measured are 7 to 20 nM (0.07 to 0.2 pmol on-column). Recoveries from culture medium to which known amounts of biphenyl and metabolites had been added were quantitative (90-103%) and the reproducibility good (interassay CV less than 5%). The assay was applied to cultures of hepatocytes derived from rabbit and from phenobarbital induced and noninduced rat. 相似文献
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56.
Moleón M Sánchez-Zapata JA Gil-Sánchez JM Ballesteros-Duperón E Barea-Azcón JM Virgós E 《Oecologia》2012,168(3):679-689
How predators impact on prey population dynamics is still an unsolved issue for most wild predator–prey communities. When
considering vertebrates, important concerns constrain a comprehensive understanding of the functioning of predator–prey relationships
worldwide; e.g. studies simultaneously quantifying ‘functional’ and ‘numerical responses’ (i.e., the ‘total response’) are
rare. The functional, the numerical, and the resulting total response (i.e., how the predator per capita intake, the population
of predators and the total of prey eaten by the total predators vary with prey densities) are fundamental as they reveal the
predator’s ability to regulate prey population dynamics. Here, we used a multi-spatio-temporal scale approach to simultaneously
explore the functional and numerical responses of a territorial predator (Bonelli’s eagle Hieraaetus fasciatus) to its two main prey species (the rabbit Oryctolagus cuniculus and the red-legged partridge Alectoris rufa) during the breeding period in a Mediterranean system of south Spain. Bonelli’s eagle responded functionally, but not numerically,
to rabbit/partridge density changes. Type II, non-regulatory, functional responses (typical of specialist predators) offered
the best fitting models for both prey. In the absence of a numerical response, Bonelli’s eagle role as a regulating factor
of rabbit and partridge populations seems to be weak in our study area. Simple (prey density-dependent) functional response
models may well describe the short-term variation in a territorial predator’s consumption rate in complex ecosystems. 相似文献
57.
CLN6, which is associated with a lysosomal storage disease, is an endoplasmic reticulum protein 总被引:4,自引:0,他引:4
Mole SE Michaux G Codlin S Wheeler RB Sharp JD Cutler DF 《Experimental cell research》2004,298(2):399-406
The neuronal ceroid lipofuscinoses (NCLs) are severe inherited neurodegenerative disorders affecting children. In this disease, lysosomes accumulate autofluorescent storage material and there is death of neurons. Five types of NCL are caused by mutations in lysosomal proteins (CTSD, CLN1/PPT1, CLN2/TTPI, CLN3 and CLN5), and one type is caused by mutations in a protein that recycles between the ER and ERGIC (CLN8). The CLN6 gene underlying a variant of late infantile NCL (vLINCL) was recently identified. It encodes a novel 311 amino acid transmembrane protein. Antisera raised against CLN6 peptides detected a protein of 30 kDa by Western blotting of human cells, which was missing in cells from some CLN6 deficient patients. Using immunofluorescence microscopy, CLN6 was shown to reside in the endoplasmic reticulum (ER). CLN6 protein tagged with GFP at the C-terminus and expressed in HEK293 cells was also found within the ER. Investigation of the effect of five CLN6 disease mutations that affect single amino acids showed that the mutant proteins were retained in the ER. These data suggest that CLN6 is an ER resident protein, the activity of which, despite this location, must contribute to lysosomal function. 相似文献
58.
Tuckerman JR Zhao Y Hewitson KS Tian YM Pugh CW Ratcliffe PJ Mole DR 《FEBS letters》2004,576(1-2):145-150
59.
Schlemminger I Mole DR McNeill LA Dhanda A Hewitson KS Tian YM Ratcliffe PJ Pugh CW Schofield CJ 《Bioorganic & medicinal chemistry letters》2003,13(8):1451-1454
Analogues of the naturally occurring cyclic hydroxamate dealanylalahopcin, which is an inhibitor of procollagen prolyl-4-hydroxylase, were synthesised and shown to be inhibitors of the human hypoxia-inducible factor prolyl hydroxylases. 相似文献
60.
The primary structure of the J chain from a human Waldenstr?ms IgM protein has been determined using a combination of automated and conventional Edman degradative procedures. Eighty-five percent of the sequence was established with peptides isolated from tryptic digests of carboxyamidomethylated and citraconylated J chain, many of which were sequenced completely. Alignment of the tryptic fragments was achieved with peptides generated by chymotrypsin and limited acid hydrolyses. The j chain consits of 129 amino acids and a single oligosaccharide structure linked to asparagine at positon 43 of the sequence. The molecular weight, including 7.5% carbohydrate by weight, is 16 422. The location and arrangement of three half-cystines could be deduced from previous studies, whereas the pairing of the remaining five disulfide bonds still needs to be clarified. 相似文献