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11.
We have studied the effect of resveratrol on lipoperoxidation and antioxidant enzyme activity level in the brain of healthy
rats. When intraperitoneally administered, resveratrol significantly and dose dependently decreased brain malondialdehyde
level. Resveratrol also increased in a dose-dependent way brain superoxide dismutase, catalase and peroxidase activities.
Optimal effect on antioxidant enzyme and lipoperoxidation products were obtained with resveratrol concentration of 12.5 mg/kg
body wt. Native polyacrylamide gel electrophoresis analysis of antioxidant isoenzymes revealed that resveratrol up regulated
at least two acidic superoxide dismutase isoforms called A1 and A2, two basic isoforms called B1 and B2. Resveratrol also up regulated two catalase isoforms and a broad peroxidase band corresponding to several isoforms. All these
findings suggest that resveratrol is able to cross the blood brain barrier and exerts potent antioxidant features. Resveratrol
also exerts neuroprotective properties by up regulating several detoxifying enzymes, most of which are iron proteins. 相似文献
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Angelo Livolsi Nathalie Niederhoffer Nassim Dali-Youcef Caroline Rambaud Catherine Olexa Walid Mokni Jean-Pierre Gies Pascal Bousquet 《PloS one》2010,5(3)
Background
Sudden infant death syndrome (SIDS) remains the leading cause of death among infants less than 1 year of age. Disturbed expression of some neurotransmitters and their receptors has been shown in the central nervous system of SIDS victims but no biological abnormality of the peripheral vago-cardiac system has been demonstrated to date. The present study aimed to seek vago-cardiac abnormalities in SIDS victims. The cardiac level of expression of muscarinic receptors, as well as acetylcholinesterase enzyme activity were investigated.Methodology/Principal Findings
Left ventricular samples and blood samples were obtained from autopsies of SIDS and children deceased from non cardiac causes. Binding experiments performed with [3H]NMS, a selective muscarinic ligand, in cardiac membrane preparations showed that the density of cardiac muscarinic receptors was increased as shown by a more than doubled Bmax value in SIDS (n = 9 SIDS versus 8 controls). On average, the erythrocyte acetylcholinesterase enzyme activity was also significantly increased (n = 9 SIDS versus 11 controls).Conclusions
In the present study, it has been shown for the first time that cardiac muscarinic receptor overexpression is associated with SIDS. The increase of acetylcholinesterase enzyme activity appears as a possible regulatory mechanism. 相似文献14.
Abdellatif Chakar Ridha Mokni Philippe Chappuis Jean-Louis Mahu Philip A. Walravens Fanny Bleiberg-Daniel Patrice Therond Jean Navarro Daniel Lemonnier 《Biological trace element research》1993,36(1):25-33
Plasma selenium (Se) concentration and erythrocyte glutathione peroxidase activity (GPx) were assessed in a population of
healthy preschool children two to five years old, residing in the city of Paris. In the 118 subjects, mean (±SD) plasma Se
concentration was 62.10 ±13.96 μg/L, and mean GPx activity was 23.58±8.52 U/g Hb. Mean plasma Se of male children was significantly
(p=0.001) higher (12%) than levels of girls. Plasma selenium levels were not correlated with erythrocyte GPx activity. Children
from Mediterranean origin had a slightly lower erythrocyte GPx activity (p<0.05) than children from other regions. Mean plasma Se concentration of this group corresponded to the lower limit of intervals,
which characterizes geographical regions of intermediate selenium concentrations. 相似文献
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Ben Salem Nesrine Boussetta Sami de Rojas Itziar Moreno-Grau Sonia Montrreal Laura Mokni Narjes Mahmoud Imene Younes Samia Daouassi Nizar Frih-Ayed Mahbouba Hammami Afef Ben Ammar Elgaaied Amel Ruiz Agustín Cherni Lotfi 《Molecular biology reports》2022,49(3):1687-1700
Molecular Biology Reports - Alzheimer’s disease (AD) is the most common neurodegenerative disorder in humans and presents a major health problem throughout the world. The etiology of AD is... 相似文献
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Mokni W Keravis T Etienne-Selloum N Walter A Kane MO Schini-Kerth VB Lugnier C 《PloS one》2010,5(12):e14227
Left ventricular hypertrophy leads to heart failure and represents a high risk leading to premature death. Cyclic nucleotides (cAMP and cGMP) play a major role in heart contractility and cyclic nucleotide phosphodiesterases (PDEs) are involved in different stages of advanced cardiac diseases. We have investigated their contributions in the very initial stages of left ventricular hypertrophy development. Wistar male rats were treated over two weeks by chronic infusion of angiotensin II using osmotic mini-pumps. Left cardiac ventricles were used as total homogenates for analysis. PDE1 to PDE5 specific activities and protein and mRNA expressions were explored.Rats developed arterial hypertension associated with a slight cardiac hypertrophy (+24%). cAMP-PDE4 activity was specifically increased while cGMP-PDE activities were broadly increased (+130% for PDE1; +76% for PDE2; +113% for PDE5) and associated with increased expressions for PDE1A, PDE1C and PDE5A. The cGMP-PDE1 activation by Ca(2+)/CaM was reduced. BNP expression was increased by 3.5-fold, while NOX2 expression was reduced by 66% and AMP kinase activation was increased by 64%. In early cardiac hypertrophy induced by angiotensin II, all specific PDE activities in left cardiac ventricles were increased, favoring an increase in cGMP hydrolysis by PDE1, PDE2 and PDE5. Increased cAMP hydrolysis was related to PDE4. We observed the establishment of two cardioprotective mechanisms and we suggest that these mechanisms could lead to increase intracellular cGMP: i) increased expression of BNP could increase "particulate" cGMP pool; ii) increased activation of AMPK, subsequent to increase in PDE4 activity and 5'AMP generation, could elevate "soluble" cGMP pool by enhancing NO bioavailability through NOX2 down-regulation. More studies are needed to support these assumptions. Nevertheless, our results suggest a potential link between PDE4 and AMPK/NOX2 and they point out that cGMP-PDEs, especially PDE1 and PDE2, may be interesting therapeutic targets in preventing cardiac hypertrophy. 相似文献
17.
Mehrzia M Ferid L Mohamed A Ezzedine A 《Indian journal of biochemistry & biophysics》2006,43(6):386-390
Garlic has been extensively used as a medicinal plant. Most of its numerous beneficial effects such as antioxidant, antibacterial, antitumoral involve sulfur-derived amino acids. In the present work, we reevaluated the acute effects of aqueous extract of garlic on plasma glucose and cholesterol levels in normal rats. Control (vehicle H2O) or garlic extract-treated group at 100-120 mg protein/kg body wt were intraperitoneally injected (IP) and glucose, cholesterol, insulin and nitric oxide metabolites levels were determined after a short-term duration of 6 h. We confirmed that garlic contained an active fraction, exerting both glucose and cholesterol-lowering activity. The glucose-lowering effect was triggered by an increase in insulinemia. Preliminary study indicated that the active agent was different from S-allyl-cysteine-sulfoxide, the active principle implicated in hypoglycaemic and hypolipidemic effects of garlic or arginine. The mechanism of action seemed to involve nitric oxide (NO), which increased time and dose-dependently. The garlic effects were abolished by diphenyleneiodonium chloride (DPI = 1 mg/kg body wt), a specific inhibitor of NO production, suggesting the involvement of constitutive nitric oxide synthase. 相似文献
18.
Abdellatif Chakar Ridha Mokni Philip A. Walravens Philippe Chappuis Fanny Bleiberg-Daniel Jean-Louis Mahu Daniel Lemonnier 《Biological trace element research》1993,38(1):97-106
Plasma zinc, copper, and parameters of growth were measured in a group of 116 French preschool children, 2–5 yr-old from low-income
households. Participants were selected on the basis of Z-scores of weight for height (WHZ) and height for age (HAZ). Zinc
and copper concentrations of children with growth impairment (GI), defined by a WHZ and/or HAZ< −1 Z-score, were compared
to those of age, sex, and ethnic origin matched controls (WHZ and HAZ >−1 Z-score). Mean (±SD) plasma zinc concentration was
12.58±1.84 μmol/L in the GI group, and 13.27±1.98 μmol/L in the controls. The difference of the means of paired samples was
0.69±2.34, and by pairedt-test the significance reachedp=0.028. This effect was primarily a result of the weight retarded group (WHZ <−1 Z-score,p<0.009) and to the girls (p<0.05). There were no significant differences in plasma copper concentrations between groups. These results suggest the presence
of marginal zinc deficiency in French preschool children with low weight for height Z-scores. 相似文献
19.
Aoun K Chahed MK Mokni M Harrat Z Bouratbine A 《Archives de l'Institut Pasteur de Tunis》2003,80(1-4):53-56
The microscopic study of the dermal smears of 62 cases of cutaneous leishmaniose, 27 infected by Leishmania (L.) infantum and 35 by L. major, showed that the amastigotes of L. infantum are meaningfully smaller (p < 0.001). This criteria is a simple pary alternative to distinguish these 2 species which have completely different epidemiology, recovery delay and prophylactic dispositions. 相似文献
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