Effects of sulfur dioxide on pore populations of canine tracheal epithelium

The bioelectric and barrier properties of the tracheal epithelium in nose-breathing dogs and in dogs that had been exposed for 75 min to compressed air or to two high concentrations of SO2 were measured and compared. We also studied tissues that had been treated with chloroform. Based on a model of restrictive diffusion we demonstrated heteropores (6 and 250 A) in the control tissues. Bioelectric changes due to 100-ppm SO2 were minimal. After exposure to 500 ppm SO2, adverse changes in the bioelectric properties were focal; they were marked in 8 out of 12 animals but were less striking in the other 4. Nonelectrolyte permeability increased with an increase in SO2 concentrations. Small pores were still present in the tissues severely affected by SO2 but they were absent in chloroform-treated tissues. Scanning electron microscopy of tissues from animals exposed to 500 ppm SO2 showed that in the same dog tissue appearance varied from normal to one of repair (normal bioelectric properties) or one of marked exfoliation of ciliated cells (abnormal bioelectric measurements).     

Cytokinin autonomy in tissue cultures of phaseolus: a genotype-specific and heritable trait

Intra- and interspecific differences in cytokinin requirement were detected in callus cultures of Phaseolus vulgaris L. and P. lunatus L. Of the ten genotypes of P. vulgaris tested in the present study, one required cytokinin for callus growth, six exhibited some to moderate growth on cytokinin-free medium, and the remaining three grew uniformly in the absence of cytokinin. In contrast, six of the P. lunatus genotypes were strictly cytokinin-dependent, while four genotypes displayed irregular amount of callus growth on cytokinin-free medium. The genotype-specific behavior of Phaseolus callus tissues was independent of the tissue of origin and the time in culture. The inheritance of the cytokinin requirement of Phaseolus tissue cultures was studied in hybrid tissues resulting from crosses between a strictly cytokinin-dependent genotype (P.I. 200960) and two independent genotypes (cv. G 50 and P.I. 286303) of P. vulgaris. Fresh weights of hybrid tissues on cytokinin-free medium were intermediate between and significantly different from the parental tissues. No differences were found between reciprocal hybrids. These results suggest that cytokinin autonomy in tissue cultures of P. vulgaris is a genetic trait under nuclear control. Both parental and intermediate phenotypes were recovered in the F₂ progeny. The frequency distribution of cytokinin-dependent progeny in F₂ and backcross populations indicates that the cytokinin requirement of P. vulgaris callus tissue may be regulated by one set of alleles.     

The effects of glucose and oxygen on the cytochromes and metabolic activity of yeast batch cultures. II. Candida utilis

Candida utilis was grown in batch culture with and without oxygen control. The concentrations of A-, B-, and C-type cytochromes were found to vary with the initial glucose concentration, with the dissolved oxygen concentration, and with time. A-type was the most sensitive. After glucose was essentially exhausted, the yeast catabolized ethanol, if it had been growing in a relatively low initial glucose concentration, or non-glucose carbohydrate, including some of that previously accumulated within the cell, if it had been growing in a high initial glucose concentration. This difference in metabolic pattern could explain why cytochrome derepression was initiated soon after glucose uptake ceased only if initial glucose had been relatively low. The effects of glucose and dissolved oxygen concentrations on yeast cytochromes and respiratory activity are discussed.     

粘虫的发育和成活与环境湿度的关系——Ⅰ.卵和一龄幼虫

在25℃中,相对湿度为20—100%的范围内,卵的孵化率受湿度的影响不明显,在32℃中,相对湿度为40-100%的范围内,湿度对卵孵化率的影响亦不显著,但当相对湿度降至20%时,孵化率即有明显的下降;在25℃及32℃中,相对湿度对卵的发育速度有明显的影响,随相对湿度的增加,发育加快,二者呈直线正相关。 卵期湿度对一龄幼虫的成活及发育有明显的影响,在32℃中,随着卵期湿度的增加,幼虫成活率提高,二者呈正相关;在25℃及60%以下的相对湿度中,亦获得了相似的结果;25℃及32℃中,一龄幼虫的发育速率与卵期相对湿度呈正相关,随卵期湿度的增加,发育加快。 一龄幼虫期的湿度条件,对一龄幼虫有明显的影响,在25℃中,幼虫的成活率和发育速率与相对湿度均呈正相关,在32℃中,这种影响更为显著,当湿度降低到40%时,全部幼虫均不能存活,在60%以上的相对湿度中,成活率和发育速率均随湿度的提高而增加。     

望谟县蚊类初步调查报告

望谟县位于我省的最南部,在北纬25°—25°30′,东经106°—106°20′区内,南面隔红水河与广西呲隣。1958年3—5月间我们在该县南端的蔗乐板、陈乐康等乡迸行疟疾流行病学调查的同寸,对当地蚊虫孳生与栖息场所进行了全面的普查。据蔗乐水文站记录:当地海拔为400米左右,1953—1957年的年平均温度为20.16℃,月平均温度最低为11.52℃(1月),最高为27.08℃(7     

牵拉猫左心房所致的肾效应

本实验在50只麻醉猫中研究了牵拉左心房(LAS)对尿量(UV)、尿钠(U_(Na)V)和尿钾(U_KV)排出量的影响。在迷走神经完整的动物,LAS 导致 UV,U_(Na)V 和 U_KV(P<0.001)明显增加。切断迷走神经后 LAS 仍能使 UV,U_(Na)V 有所增加(p<0.01),但增加量明显低于迷走神经完整的动物(P<0.005)。在迷走神经完整的猫滴注肝素(10U/min/mg)后,LAS 也能引起 UV,U_(Na)V 和 U_KV 的增加,但增值明显低于没有滴注肝素的猫。在迷走神经切除后滴入肝素,则 LAS 的肾效应消失(p>0.05)。切断左侧肾神经后,LAS 引起神经完整肾尿量和尿钠排出显著增加(P<0.001)。而去神经肾对 LAS 的效应虽减弱,但其增值仍有显著性。两侧肾效应的差异,在统计学上是显著的(P<0.05)。上述结果说明,LAS 在麻醉猫中能引起尿量,尿钠和尿钾明显增加,这些效应是神经反射和体液机制共同作用的结果。     

Phorbol ester dissociates endothelin-stimulated phosphoinositide hydrolysis and arachidonic acid release in vascular smooth muscle cells

Endothelin-stimulated [3H]-inositol phosphate formation and [3H]-arachidonic acid release were measured in cultured vascular smooth muscle cells from rabbit renal artery. Both responses were partially inhibited by pretreatment with pertussis toxin, indicating the involvement of pertussis toxin-sensitive guanine nucleotide binding regulatory proteins in the coupling processes. Pretreatment with the phorbol ester PMA inhibited endothelin-stimulated [3H]-inositol phosphate formation, but potentiated endothelin-stimulated [3H]-arachidonic acid release, suggesting that these two coupling processes occur in a parallel and independent manner in vascular smooth muscle cells.     

Water Relations and Cell Wall Elasticity Quantities in Phaseolus vulgaris Leaves

The investigations were designed to test osmotic adjustment,cell wall bulk elastic modulus and stomatal behaviour duringand after water stress and rewatering in the primary and firsttrifoliolate leaf of Phaseolus vulgaris. Leaf water relationsquantities fully recovered after rewatering within a few hours;diffusion resistance to vapour flow, however, required 6 h.Leaf growth recovery was considerably delayed. Osmotic adjustmentwas absent during water stress in both the primary and the firsttrifoliolate leaf. The bulk elastic modulus (_v), however, waslower for the primary leaf (higher elasticity) than for thetrifoliolate leaves. These two types of leaves differed in theirdrought resistance in that the primary leaf exhibited wiltingat the end of the stress period (7 d) while the trifoliolateleaf remained relatively turgid. The bulk elastic modulus ofthe cell wall changed almost proportionally with the turgorpressure (_p). The structure coefficient (), an indicator forthe intensity of change of the bulk elastic modulus with turgorwas higher for the primary than for the first trifoliolate leaf.The leaf diffusion resistance (r), below the turgor loss point,changed proportionally with the solute potential with very similarregression lines for the relation of (r) versus RWC ¹. The datasuggest that greater drought resistance of the first trifoliolateleaf is related to a decreased bulk elastic modulus, but notto osmotic adjustment nor to differences in stomatal resistanceduring water stress. Key words: Phaseolus vulguris, Water stress, Recovery, Cell wall elasticity