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排序方式: 共有162条查询结果,搜索用时 46 毫秒
81.
Jordi Corominas Jorge AP Marchesi Anna Puig-Oliveras Manuel Revilla Jordi Estellé Estefania Alves Josep M Folch Maria Ballester 《遗传、选种与进化》2015,47(1)
Background
In previous studies on an Iberian x Landrace cross, we have provided evidence that supported the porcine ELOVL6 gene as the major causative gene of the QTL on pig chromosome 8 for palmitic and palmitoleic acid contents in muscle and backfat. The single nucleotide polymorphism (SNP) ELOVL6:c.-533C > T located in the promoter region of ELOVL6 was found to be highly associated with ELOVL6 expression and, accordingly, with the percentages of palmitic and palmitoleic acids in longissimus dorsi and adipose tissue. The main goal of the current work was to further study the role of ELOVL6 on these traits by analyzing the regulation of the expression of ELOVL6 and the implication of ELOVL6 polymorphisms on meat quality traits in pigs.Results
High-throughput sequencing of BAC clones that contain the porcine ELOVL6 gene coupled to RNAseq data re-analysis showed that two isoforms of this gene are expressed in liver and adipose tissue and that they differ in number of exons and 3’UTR length. Although several SNPs in the 3’UTR of ELOVL6 were associated with palmitic and palmitoleic acid contents, this association was lower than that previously observed with SNP ELOVL6:c.-533C > T. This SNP is in full linkage disequilibrium with SNP ELOVL6:c.-394G > A that was identified in the binding site for estrogen receptor alpha (ERα). Interestingly, the ELOVL6:c.-394G allele is associated with an increase in methylation levels of the ELOVL6 promoter and with a decrease of ELOVL6 expression. Therefore, ERα is clearly a good candidate to explain the regulation of ELOVL6 expression through dynamic epigenetic changes in the binding site of known regulators of ELOVL6 gene, such as SREBF1 and SP1.Conclusions
Our results strongly suggest the ELOVL6:c.-394G > A polymorphism as the causal mutation for the QTL on pig chromosome 8 that affects fatty acid composition in pigs.Electronic supplementary material
The online version of this article (doi:10.1186/s12711-015-0111-y) contains supplementary material, which is available to authorized users. 相似文献82.
Moiseev S. A. Pukhov A. M. Mikhailova E. A. Gorodnichev R. M. 《Journal of Evolutionary Biochemistry and Physiology》2022,58(1):88-97
Journal of Evolutionary Biochemistry and Physiology - In motor synergy studies, modern approaches typically include the consideration of synergistic effects at one or, less often, two levels of the... 相似文献
83.
Human Physiology - The experimental results of human muscle spatiotemporal organization, kinematic synergies, and causes of their variability in locomotions of different intensity were described.... 相似文献
84.
GV Pavlova AA Vergun EY Rybalkina PR Butovskaya AP Ryskov 《Cell cycle (Georgetown, Tex.)》2015,14(2):200-205
Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells demonstrated increased DNA instability. RAPD fragments from different cell lines after different number of passages were cloned and sequenced. The chromosomal localization of these fragments was identified and single-nucleotide variations in RAPD fragments isolated from cell lines after 8–12 passages were revealed. Some of them had permanent localization, while most variations demonstrated random distribution and can be considered as de novo mutations. 相似文献
85.
Tara Chapman Fedor Moiseev Victor Sholukha Stéphane Louryan Marcel Rooze Patrick Semal Serge Van Sint Jan 《Comptes Rendus Palevol》2010,9(6-7):445-454
A major problem of fossil hominid analysis is a lack of complete specimens. Many individual specimens have been damaged by the effects of diagenesis and excavation. Significant advances in the field of three dimensional image processing (3D) have enabled the creation of accurately scaled reconstructions of individual fossil bones using mirrored parts of the same fossil bone or human/fossil hominid equivalents. This study presents, for the first time, a method to reconstruct a 3D virtual model of the lower limb of the Neandertal using different bones from different fossil remains (Spy II, Neandertal 1 and Kebara 2) and integrating them into a single model of the Neandertal lower limb. A biomechanical analysis of the model was performed, including computer graphics visualization of the results, motion displacement graphs and muscle moment arms. The overall method has been implemented into an open-source customized software (lhpFusionBox) developed for the biomechanical study of the musculoskeletal system. 相似文献
86.
A V Lichtenstein V L Moiseev M M Zaboykin 《Journal of biochemical and biophysical methods》1989,18(1):77-83
Transfer of DNA (from 0.1 to 10 micrograms) from diluted solutions of variable volumes (1-10 ml) and various composition (2 M NaCl; 4 M LiCl, 8 M urea; 4 M CsCl; 20% sucrose) to nitrocellulose or nylon membranes was achieved with the use of hydroxyapatite. This absorbent that binds nucleic acids effectively and independently of ionic strength and composition of solution (except for chelators and phosphate ions) easily dissolves in small volumes of acids (for example, in 10% TCA). This phenomenon provides the opportunity to deliver the acid-insoluble precipitates to membrane filters. After alkaline denaturation on the filter followed by a fixation step (baking or UV irradiation for nitrocellulose or nylon filters, respectively), DNA hybridizes effectively with nick-translated DNA probes. The method is simple, reproducible, sensitive, and useful for working with diluted DNA solutions containing interfering substances. 相似文献
87.
88.
David Lee Tjaart AP de Beer Roman A Laskowski Janet M Thornton Christine A Orengo 《BMC structural biology》2011,11(1):2
Background
The Midwest Center for Structural Genomics (MCSG) is one of the large-scale centres of the Protein Structure Initiative (PSI). During the first two phases of the PSI the MCSG has solved over a thousand protein structures. A criticism of structural genomics is that target selection strategies mean that some structures are solved without having a known function and thus are of little biomedical significance. Structures of unknown function have stimulated the development of methods for function prediction from structure. 相似文献89.
S Çolakoğlu A Aktaş S Raimondo AP Türkmen BZ Altunkaynak E Odacı 《Biotechnic & histochemistry》2014,89(2):136-144
We investigated the effects of diclofenac sodium (DS) on development of the optic nerve in utero. Pregnant female rats were separated into three groups: control, saline treated and DS treated. Offspring of these animals were divided into 4-week-old and 20-week-old groups. At the end of the 4th and 20th weeks of postnatal life, the animals were sacrificed, and right optic nerves were excised and sectioned for ultrastructural and stereological analyses. We demonstrated that both DS and saline produced structural and morphometric changes in the total axon number and density of axons, but decreased the myelin sheath thickness in male optic nerves. All ultrastructural and morphometric features were well developed in 20-week-old rats. We showed that development of the optic nerve continues during the early postnatal period and that some compensation for exposure to deleterious agents in utero may occur during early postnatal life. 相似文献
90.
Network analysis of temporal functionalities of the gut induced by perturbations in new-born piglets
Nirupama Benis Dirkjan Schokker Maria Suarez-Diez Vitor AP Martins dos Santos Hauke Smidt Mari A Smits 《BMC genomics》2015,16(1)