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751.
Sohair R. Fahmy Amel M. Soliman Mervat El Ansary Samah Abd Elhamid Heba Mohsen 《Tissue & cell》2017,49(3):369-375
Background
Acute kidney injury (AKI) is a common clinical problem raising the urgent needs to develop new strategies for treatment. The present study investigated the therapeutic potential of human umbilical cord – mesenchymal stem cells (HUC-MSCs) transplantation against renal ischemia/reperfusion injury (IRI) in rats.Methods
Twenty four male Wistar rats were assigned into two main groups, sham group (control group) and I/R group. I/R group was injected in the tail vein with either phosphate buffer saline (PBS) or HUC-MSCs.Results
The HUC-MSCs improved kidney injury induced by I/R as demonstrated by enhancement of the kidney function via decreasing serum levels of creatinine, urea and uric acid. The therapeutic efficacy of HUC-MSCs were found to be mediated through anti-oxidant activity as indicated by significant reduction in total malondialdehyde (MDA) and significant increment in the levels of reduced glutathione (GSH), catalase (CAT) and glutathione-S-transferase (GST).Conclusion
The present work suggests that HUC-MSCs may be an effective therapeutic agent against renal IRI. The recorded data showed improvement of renal functions and urine albumin in HUC-MSCs than IRI group with positive antioxidant efficacy of HUC-MSCs through scavenging free radicals and supporting the antioxidant enzymes. 相似文献752.
Mohsen Sargolzaei Mahdi Afshar Hossein Nikoofard 《Russian Journal of Bioorganic Chemistry》2017,43(4):435-442
DNA binding position and binding affinity of drugs are important information that helps medicinal chemists in synthesis of new drugs. We used molecular docking and molecular dynamics simulation to reveal binding strength of thieno[2,3-b]benzo[1,8]naphthyridine derivatives to DNA. Molecular docking showed that molecules with more steric hindrance select groove position in DNA structure. Other molecules are intercalated between base pairs of GC and AT. Restrained electrostatic potential (RESP) charges, root mean square deviation (RMSD), and total potential analyses were performed. RMSD and total potential analyses showed that all simulations have stability for MMGBSA analysis. Binding affinity of all drugs was derived via MMGBSA analysis. Thermodynamics analysis showed that binding affinity of groove binding drugs is less than that of intercalating ones. Also, it was found that a linear relationship exists between RESP charges and ΔG pred. Additionally, our results demonstrated the highest affinity for molecules carrying substituent groups of–OCH3 and–CH3. 相似文献
753.
Frank J.A. van Rooij Rehan Qayyum Albert V. Smith Yi Zhou Stella Trompet Toshiko Tanaka Margaux F. Keller Li-Ching Chang Helena Schmidt Min-Lee Yang Ming-Huei Chen James Hayes Andrew D. Johnson Lisa R. Yanek Christian Mueller Leslie Lange James S. Floyd Mohsen Ghanbari Santhi K. Ganesh 《American journal of human genetics》2017,100(1):51-63
754.
In this study, the opportunity to enhance the insecticidal activity of Rosmarinus officinalis essential oil was studied for effective management of the red flour beetle, Tribolium castaneum , as a stored product pest beetle. Nanoprecipitation method was used to prepare rosemary oil‐loaded nanocapsules. Bioassays were conducted at 27–30°C temperature and 70–75 % relative humidity in the dark. Fumigant toxicity of the non‐formulated oil and nanocapsules of R. officinalis were investigated at 13.20, 15.92, 19.12, 23.04, and 27.76 μL/L air after 24 and 72 h exposure and the contact toxicity of the non‐formulated oil and nanocapsules were investigated at 4.28, 3.55, 2.95, 2.45 and 2.36 μL/cm2 after 24 h exposure. The major constituents of the essential oil of rosemary were α‐Pinene, 1,8‐cineol, camphor, and cis‐verbenone. Nanocapsules presented an average size (145 ± 15 nm) (± standard error [SE]) with a polydispersity index below 0.3, a negative zeta potential (?11.0 ± 0.5 mV), and a high encapsulation efficiency (78.20 ± 0.93 %). Scanning electron microscope photomicrograph of rosemary oil‐loaded nanocapsules showed the presence of spherical nanocapsules with regular and homogeneous surfaces. In fumigant and contact toxicity, there were significant differences between non‐formulated and rosemary oil‐loaded nanocapsules in all the concentrations and times. The results suggested that nanoencapsulated essential oils from R. officinalis can be used for effective control in T. castaneum . When this technique is used, it can produce pesticides that have controlled‐release properties and reduce the concentration of the applied doses and number of applications. 相似文献
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757.
Sayed Mohammad Shafiee Atefeh Seghatoleslam Mohsen Nikseresht Seyed Vahid Hosseini Mahvash Alizadeh-Naeeni Akbar Safaei Ali Akbar Owji 《Molecular biology reports》2013,40(12):7045-7051
Colorectal cancer is the third most common cancer in the world. Ubiquitin–proteasome system has shown to be activated in colorectal and other malignancies. UBE2Q1 is a novel human gene that encodes a putative E2 ubiquitin conjugating enzyme. Here, we investigated the expression pattern of UBE2Q1 gene in cell lines and tissues from human colorectal tumors. Quantitative (q) RT-PCR were employed to evaluate the expression levels of the mRNA for UBE2Q1 in colorectal cancer cell lines (HT29/219, LS180, SW742, Caco2, HTC116, SW48, SW480 and SW1116). Expression of UBE2Q1 at the protein levels were assessed by Western blotting in cell lines as well as in 43 human colorectal tumor tissues. All cell lines tested expressed UBE2Q1 gene at the level of both mRNA and protein, with the SW1116 line representing the lowest level of expression. The cell lines HT29/219 and SW742 showed the highest levels of UBE2Q1 protein and mRNA respectively. When compared to corresponding normal tissues, malignant parts of colorectal tumors showed increased levels of UBE2Q1 immunoreactivity in 32 (74.42 %) of cases. These data suggest that UBE2Q1 is differentially expressed in colorectal cell lines and shows overexpression in colorectal tumors. 相似文献
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759.
Seghatoleslam A Nikseresht M Shafiee SM Monabati A Namavari MM Talei A Safaei A Owji AA 《Molecular biology reports》2012,39(5):5135-5141
The novel human gene, designated ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1) maps to chromosome 1q21.3. The
gene has an open reading frame corresponding to 422 amino acids and contains a RWD domain and an E2 ubiquitin conjugating
enzyme domain. Here, we investigated the expression levels of both mRNA and protein of UBE2Q1 gene in cancerous versus normal
parts of breast specimens from 26 patients. Real-time PCR data showed that the relative expression level of UBE2Q1 mRNA was
significantly greater in cancers than in non-cancerous tissues of breast specimens (Mean ± SEM, 0.064 ± 0.015 for cancers
and 0.026 ± 0.01 for noncancerous tissues, P < 0.05 Mann–Whitney test). A rabbit polyclonal antibody was generated against an amino acid sequence predicted from the DNA
sequence of UBE2Q1 gene. This antibody was used to perform Western blotting on 21 cases in our cohort of breast specimens.
Thus, 13 (61.904%) of the cases showed an increase in the UBE2Q1 immunoreactivity in their cancerous tissues as compared with
the corresponding normal tissues. This result along with the real-time PCR data shows that the novel human gene, UBE2Q1, is
expressed in human breast and may have implications for pathogenesis of breast cancer. 相似文献