全文获取类型
收费全文 | 783篇 |
免费 | 42篇 |
国内免费 | 1篇 |
出版年
2023年 | 11篇 |
2022年 | 22篇 |
2021年 | 41篇 |
2020年 | 36篇 |
2019年 | 69篇 |
2018年 | 49篇 |
2017年 | 29篇 |
2016年 | 53篇 |
2015年 | 45篇 |
2014年 | 53篇 |
2013年 | 64篇 |
2012年 | 67篇 |
2011年 | 52篇 |
2010年 | 37篇 |
2009年 | 24篇 |
2008年 | 31篇 |
2007年 | 26篇 |
2006年 | 25篇 |
2005年 | 16篇 |
2004年 | 21篇 |
2003年 | 9篇 |
2002年 | 10篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1982年 | 3篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 2篇 |
1968年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有826条查询结果,搜索用时 171 毫秒
21.
22.
Mohsen Koohestani Richard Perdriau Yves Le Dréan Mauro Ettorre Maxim Zhadobov 《Bioelectromagnetics》2020,41(5):369-381
This paper presents the design of a resonant system for in vitro studies to emulate the exposure of a monolayer of cells to a wireless power transfer system operating at 13.56 MHz. The design procedure targets a system, which maximizes the specific absorption rate (SAR) uniformity on the plane where the layer is cultured, as well as SAR efficiency (defined as SAR over the input power), within the size constraints of a standard incubator. Three resonant wireless power transfer systems with different commonly used loop/coil geometries (cylindrical with circular and square cross-sections and annular) were compared with assess the configuration maximizing the considered design criteria. The system performance in terms of reflection and transmission coefficients, as well as generated E- and H-fields, was characterized numerically and experimentally inside the incubator. Moreover, SAR was computed at the monolayer level. The system equipped with cylindrical coils with square cross-sections led to a high electromagnetic field uniformity in in vitro biological samples. In particular, the uniformities in E and SAR at the layer level were within 7.9% and 5.5%, respectively. This was achieved with the variation in H below the usually considered ±5% limit. © 2020 Bioelectromagnetics Society 相似文献
23.
Cai Gaochao Ahmed Mutez Ali Dippold Michaela A. Zarebanadkouki Mohsen Carminati Andrea 《Plant and Soil》2020,447(1-2):579-579
Plant and Soil - In the original version of this article, equations 4 and 9 unfortunately contained errors 相似文献
24.
Ghazizadeh Hamideh Rezaei Majid Avan Amir Fazilati Mohammad Pasdar Alireza Tavallaie Shima Kazemi Elham Seyedi Seyed Mohammad Reza Ferns Gordon A. Azimi-Nezhad Mohsen Ghayour-Mobarhan Majid 《Molecular biology reports》2020,47(2):867-875
Molecular Biology Reports - Metabolic syndrome (MetS) is associated with a pro-inflammatory state and endothelial dysfunction that places subjects with MetS at a higher risk of atherosclerosis.... 相似文献
25.
26.
Safaei Mohsen Mobini Gholam-Reza Abiri Ardavan Shojaeian Ali 《Molecular biology reports》2020,47(8):6207-6216
Molecular Biology Reports - Synthetic biology breakthroughs have facilitated genetic circuit engineering to program cells through novel biological functions, dynamic gene expressions, as well as... 相似文献
27.
Kornelia Neveling Lilian?A. Martinez-Carrera Irmgard H?lker Angelien Heister Aad Verrips Seyyed?Mohsen Hosseini-Barkooie Christian Gilissen Sascha Vermeer Maartje Pennings Rowdy Meijer Margot te?Riele Catharina?J.M. Frijns Oksana Suchowersky Linda MacLaren Sabine Rudnik-Sch?neborn Richard?J. Sinke Klaus Zerres R.?Brian Lowry Henny?H. Lemmink Lutz Garbes Joris?A. Veltman Helenius?J. Schelhaas Hans Scheffer Brunhilde Wirth 《American journal of human genetics》2013,92(6):946-954
Spinal muscular atrophy (SMA) is a heterogeneous group of neuromuscular disorders caused by degeneration of lower motor neurons. Although functional loss of SMN1 is associated with autosomal-recessive childhood SMA, the genetic cause for most families affected by dominantly inherited SMA is unknown. Here, we identified pathogenic variants in bicaudal D homolog 2 (Drosophila) (BICD2) in three families afflicted with autosomal-dominant SMA. Affected individuals displayed congenital slowly progressive muscle weakness mainly of the lower limbs and congenital contractures. In a large Dutch family, linkage analysis identified a 9q22.3 locus in which exome sequencing uncovered c.320C>T (p.Ser107Leu) in BICD2. Sequencing of 23 additional families affected by dominant SMA led to the identification of pathogenic variants in one family from Canada (c.2108C>T [p.Thr703Met]) and one from the Netherlands (c.563A>C [p.Asn188Thr]). BICD2 is a golgin and motor-adaptor protein involved in Golgi dynamics and vesicular and mRNA transport. Transient transfection of HeLa cells with all three mutant BICD2 cDNAs caused massive Golgi fragmentation. This observation was even more prominent in primary fibroblasts from an individual harboring c.2108C>T (p.Thr703Met) (affecting the C-terminal coiled-coil domain) and slightly less evident in individuals with c.563A>C (p.Asn188Thr) (affecting the N-terminal coiled-coil domain). Furthermore, BICD2 levels were reduced in affected individuals and trapped within the fragmented Golgi. Previous studies have shown that Drosophila mutant BicD causes reduced larvae locomotion by impaired clathrin-mediated synaptic endocytosis in neuromuscular junctions. These data emphasize the relevance of BICD2 in synaptic-vesicle recycling and support the conclusion that BICD2 mutations cause congenital slowly progressive dominant SMA. 相似文献
28.
Amir Assadieskandar Amirali Amirhamzeh Marjan Salehi Keriman Ozadali Seyed Nasser Ostad Abbas Shafiee Mohsen Amini 《Bioorganic & medicinal chemistry》2013,21(8):2355-2362
A series of 4-aryl-5-(4-(methylsulfonyl)phenyl)-2-alkylthio and 2-alkylsulfonyl-1H-imidazole derivatives were synthesized. All compounds were tested in human blood assay to determine COX-1 and COX-2 inhibitory potency and selectivity. Among the synthesized compounds, 2-alkylthio series were more potent and selective than 2-sulfonylalkyl derivatives. In molecular modeling, interaction of 2-sulfonylalkyl moiety with Arg120 in COX-1 and an extra hydrogen bond with Tyr341 in COX-2 increased the residence time of ligands in the active site in 2-sulfonylalkyl and 2-alkylthio analogs, respectively. 相似文献
29.
Behnaz Forouhar Kalkhoran Farida Behzadian Farzaneh Sabahi Mohsen Karimi Hesam Mirshahabi 《Reports of Biochemistry & Molecular Biology》2013,2(1):28-34
Background:
Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging.Methods:
In this study, PCR-based site-directed mutagenesis by the overlap extension method was used to create the point mutation converting the small-HDAg (S-HDAg) stop codon to a tryptophan codon through three stages.Results:
Sequencing confirmed the desirable mutation and integrity of the L-HDAg open reading frame. The amplicon was ligated into pcDNA3.1 and transfected to Huh7 and HEK 293 cell lines. Western blot analysis using enhanced chemiluminescence confirmed L-HDAg expression. The recombinant L-HDAg localized within the nuclei of cells as determined by immunofluorescence and confocal microscopy.Conclusion:
Because L-HDAg requires extensive post-translational modifications, the recombinant protein expressed in a mammalian system might be fully functional and applicable as a tool in HDV molecular studies, as well as in future vaccine research.Key Words: Hepatitis Delta Virus, L-HDAg, SOEing-PCR 相似文献30.