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31.
Ratnesh Kumar Tripathi Vindhya Mohindra Akanksha Singh Rajesh Kumar Rahasya Mani Mishra Joy Krushna Jena 《Journal of biosciences》2013,38(2):373-383
With an aim to study the mechanism of adaptation to acute hypoxic periods by hypoxia-tolerant catfish, Clarias batrachus, the mass-specific metabolic rate (VO2) along with its hematological parameters, metabolic response and antioxidant enzyme activities were studied. During progressive hypoxia, C. batrachus was found to be an oxyconformer and showed a steady decline in its aquatic oxygen consumption rate. When C. batrachus was exposed for different periods at experimental hypoxia level (0.98?±?0.1 mg/L, DO), hemoglobin and hematocrit concentrations were increased, along with decrease in mean cellular hemoglobin concentration, which reflected a physiological adaptation to enhance oxygen transport capacity. Significant increase in serum glucose and lactate concentration as well as lactate dehydrogenase activity was observed. Antioxidant enzymes were found to operate independently of one another, while total glutathione concentration was unaffected in any of the tissues across treatments. These observations suggested that hypoxia resulted in the development of oxidative stress and C. batrachus was able to respond through increase in the oxygen carrying capacity, metabolic depression and efficient antioxidant defense system to survive periods of acute hypoxia. 相似文献
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Dutta Nimisha Singh Rajeev K. Mohindra Vindhya Pathak Abhinav Kumar Raj Sah Priyanka Mandal Sangeeta Kaur Gurjeet Lal Kuldeep K. 《Molecular biology reports》2019,46(1):41-49
Molecular Biology Reports - In present study, single molecule-real time sequencing technology was used to obtain a validated set of microsatellite markers for application in population genetics of... 相似文献
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Sangeeta Mandal Achal Singh Priyanka Sah Rajeev K. Singh Raj Kumar Kuldeep K. Lal Vindhya Mohindra 《Journal of fish biology》2021,98(2):430-444
Silonia silondia is a commercially important fish distributed in Asian countries, which is under threat due to overexploitation. This study focuses on the morphological analysis and genetic variation of S. silondia individuals, through truss network and sequencing of two mitochondrial regions, respectively, from six wild populations of the Ganga and Mahanadi river systems in India. A total of 38 haplotypes was observed by analysing combined mitochondrial genes (cytochrome b + ATPase 6/8) in 247 individuals of S. silondia collected from six populations. Average haplotype and nucleotide diversities were 0.8508 and 0.00231, respectively. Genetic structure analysis showed the predominant cause of genetic variation to be within populations. The two clades were observed among the haplotypes and time of divergence from their most probable ancestor was estimated to be around 0.3949 mya. Analysis of combined mitochondrial genes in six populations of S. silondia resulted into three management units or genetic stocks. The truss network analysis was carried out by interconnecting 12 landmarks from digital images of specimens to identify phenotypic stocks. Sixty-five truss morphometric variables were analysed for geometric shape variation which revealed morphological divergence in River Son specimens. The present study presents molecular markers and genetic diversity data which can be critical input for conservation and management of differentiated populations and future monitoring of the genetic bottleneck. The morphological shape analysis clearly shows that variation in the insertion of adipose fin is an important parameter influencing the morphological discrimination. 相似文献
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Maria Habib W. S. Lakra Vindhya Mohindra Praveen Khare A. S. Barman Akanksha Singh Kuldeep K. Lal Peyush Punia Asif A. Khan 《Molecular biology reports》2011,38(2):841-846
Channa marulius (Hamilton, 1822) is a commercially important freshwater fish and a potential candidate species for aquaculture. The present
study evaluated partial Cytochrome b gene sequence of mtDNA for determining the genetic variation in wild populations of C. marulius. Genomic DNA extracted from C. marulius samples (n = 23) belonging to 3 distant rivers; Mahanadi, Teesta and Yamuna was analyzed. Sequencing of 307 bp Cytochrome b mtDNA fragment
revealed the presence of 5 haplotypes with haplotype diversity value of 0.763 and nucleotide diversity value of 0.0128. Single
population specific haplotype was observed in Mahanadi and Yamuna samples and 3 haplotypes in Teesta samples. The analysis
of data demonstrated the suitability of partial Cytochrome b sequence in determining the genetic diversity in C. marulius population. 相似文献
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Abdul Muneer PM Gopalakrishnan A Musammilu KK Mohindra V Lal KK Basheer VS Lakra WS 《Molecular biology reports》2009,36(7):1779-1791
Random amplified polymorphic DNA (RAPD) and microsatellite markers were applied to evaluate the genetic variation in endemic
and endangered yellow catfish, Horabagrus
brachysoma sampled from three geographic locations of Western Ghat, South India river systems. In RAPD, of 32 10-mer RAPD primers screened
initially, 10 were chosen and used in a comparative analysis of H. brachysoma collected from Meenachil, Chalakkudy and Nethravathi River systems. Of the 124 total RAPD fragments amplified, 49 (39.51%)
were found to be shared by individuals of all 3 populations. The remaining 75 fragments were found to be polymorphic (60.48%).
In microsatellites, six polymorphic microsatellite loci were identified by using primers developed for Pangasius
hypophthalmus, Clarias
macrocephalus and Clarias
gariepinus. The identified loci were confirmed as microsatellite by sequencing after making a clone. The nucleotide sequences of 6 loci
were published in NCBI genbank. The number of alleles across the six loci ranged from 4 to 7 and heterozygosities ranged from
0.07 to 0.93. The mean number of alleles and effective number of alleles per locus were 5.00 and 3.314, respectively. The
average heterozygosity across all investigated samples was 0.72, indicating a significant deficiency of heterozygotes in this
species. RAPD and microsatellite methods reported a high degree of gene diversity and genetic distances depicted by UPGMA
dendrograms among the populations of H. brachysoma. 相似文献
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A normalized cDNA library from spleen of Indian catfish, Clarias batrachus, was constructed with a redundancy factor of 2.29. A total of 2045 clones from the library were single-pass sequenced, which generated 1937 high quality ESTs with an average read length of approximately 700 bp. Based on sequence similarities, 65 ESTs were found to be associated with immune functions, which were mainly associated with response to stress, response to chemical stimulus, cellular response to stimulus, response to external stimulus, immune response and regulation of response to stimulus. The immune-relevant gene for CD141, thrombomodulin, has been identified in Teleosts for the first time. Six EST-SSRs and three SNPs were found associated with eight immune-relevant genes. These markers associated with important immune genes would be useful for the identification of trait associated alleles for marker-assisted selection. The identification of the putative immune-related genes provides a meaningful framework to understand the Indian catfish immune system and defense mechanisms. 相似文献
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Mohindra V Singh A Barman AS Tripathi R Sood N Lal KK 《Molecular biology reports》2012,39(5):5921-5931
Clarias batrachus, an Indian catfish species, is endemic to the Indian subcontinent and potential cultivable species. The genomic resources
in C. batrachus in the form of ESTs containing microsatellite repeats (EST-SSR) and single nucleotide polymorphisms (SNPs) that are associated
with the expressed genes from spleen were mined. From a total of 1,937 ESTs generated, 1,698 unique sequences were obtained,
out of which 221 EST-SSRs were identified and 54% could be functionally annotated by similarity searches. A total of 23 contigs
containing 3 or more ESTs were found to contain 31 SNP loci, out of which 8 ESTs showed similarity to genes of known function
and 1 for hypothetical protein. Nine ESTs with SSRs and/or SNPs identified in this study were reported to be associated with
diseases in human and animals. These identified loci can be developed into markers in C. batrachus, which can be useful in linkage mapping, comparative genomics studies and for its genetic improvement programmes. 相似文献
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Al-Abed Y Dubrovsky L Ruzsicska B Seepersaud M Bukrinsky M 《Bioorganic & medicinal chemistry letters》2002,12(21):3117-3119
Arylene bis(methylketone) compounds specifically block nuclear translocation of the HIV-1 pre-integration complex by forming Schiff-base adducts with contiguous lysines within nuclear localization signal. 相似文献