全文获取类型
收费全文 | 358篇 |
免费 | 13篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 11篇 |
2020年 | 7篇 |
2019年 | 6篇 |
2018年 | 7篇 |
2017年 | 4篇 |
2016年 | 11篇 |
2015年 | 13篇 |
2014年 | 10篇 |
2013年 | 12篇 |
2012年 | 25篇 |
2011年 | 20篇 |
2010年 | 23篇 |
2009年 | 18篇 |
2008年 | 16篇 |
2007年 | 21篇 |
2006年 | 17篇 |
2005年 | 15篇 |
2004年 | 12篇 |
2003年 | 19篇 |
2002年 | 12篇 |
2001年 | 9篇 |
2000年 | 3篇 |
1999年 | 9篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1980年 | 2篇 |
1974年 | 2篇 |
1972年 | 1篇 |
1970年 | 3篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
1964年 | 3篇 |
排序方式: 共有371条查询结果,搜索用时 15 毫秒
11.
Ray Mohapatra Amrita Lakshmanan Divya Mahesh Ramatchandirane Suchiang Kitlangki Jeevaratnam Kadirvelu 《International journal of peptide research and therapeutics》2021,27(3):1783-1797
International Journal of Peptide Research and Therapeutics - Biofilm forming pathogens are among the major causes of hospital-acquired infections and are not much affected by antibiotic treatment.... 相似文献
12.
13.
Sowmya H. Reddy Sumanth K. Kambalimath Rajesh K. Singhal Manjunath K. Chikkakariyappa Raveendran Muthurajan Mavinahalli P. Rajanna Rohini Sreevathsa Amitha M. Sevanthi Trilochan Mohapatra Neelamraju Sarla Viswanathan Chinnusamy Gopala S. Krishnan Ashok K. Singh Nagendra K. Singh Rameshwar P. Sharma Sreeman M. Sheshshayee 《Physiologia plantarum》2019,166(2):596-611
14.
Latha Gunturu Manju Raman K. V. Lima John Milton Pattanayak Debasis Singh Ashok K. Chinnusamy Viswanathan Bansal Kailash C. Sambasiva Rao K. R. S. Mohapatra Trilochan 《Plant Cell, Tissue and Organ Culture》2019,137(1):173-179
Plant Cell, Tissue and Organ Culture (PCTOC) - Adventitious root (AR) culturing is an effective approach for obtaining bioactive compounds from the endangered plant species of Oplopanax elatus... 相似文献
15.
p27Kip1 regulates T cell proliferation 总被引:6,自引:0,他引:6
Our studies addressed the mechanism by which serum acts in conjunction with T cell receptor (TCR) agonists to promote the proliferation of primary splenic T cells. When added to resting splenocytes, TCR agonists initiated G(0)/G(1) traverse and activated cyclin D3-cdk6 complexes in a serum-independent manner. On the other hand, both TCR agonists and 10% serum were required for the activation of cyclin E-cdk2 and cyclin A-cdk2 complexes and the entry of cells into S phase. Serum facilitated cdk2 activation by maximizing the extent and extending the duration of the TCR-initiated down-regulation of the cdk2 inhibitor, p27(Kip1). Although p27(Kip1) levels were reduced (albeit submaximally) in cells stimulated in serum-deficient medium, nearly all of the cdk2 complexes in these cells contained p27(Kip1). In contrast, in cells receiving TCR agonist and 10% serum, little if any p27(Kip1) was present in cyclin-cdk2 complexes. Unlike wild-type splenocytes, p27(Kip1)-null splenocytes did not require serum for cdk2 activation or S phase entry whereas loss of the related cdk2 inhibitor, p21(Cip1), did not override the serum dependence of these responses. We also found that cdk2 activation was both necessary and sufficient for maximal expression of cdk2 protein. These studies provide a mechanistic basis for the serum dependence of T cell mitogenesis. 相似文献
16.
17.
Zhang W Singam R Hellermann G Kong X Juan HS Lockey RF Wu SJ Porter K Mohapatra SS 《Genetic vaccines and therapy》2004,2(1):8-10
BACKGROUND: The need for safe and effective treatment of dengue virus (DEN), a class A agent that causes dengue hemorrhagic fever/dengue shock syndrome, has been a critical global priority. An effective vaccine for DEN is not yet available. In this study the possibility of attenuating DEN infection using adeno-associated virus (AAV)-encoded short interfering RNAs (siRNA) was examined in Vero cells and human dendritic cells (DCs). METHODS: A cassette encoding siRNA targeted to a 3' untranslated sequence common to all DEN serotypes was designed and tested for its ability to attenuate DEN infection by use of AAV delivery. RESULTS: Vero cells or DCs infected with AAV-siRNA showed a significant, dose-dependent reduction in DEN infection. Treatment of DCs with AAV-siRNA also decreased the DEN-induced apoptosis of DCs and did not induce significant inflammation. CONCLUSION: These results demonstrate that AAV-mediated siRNA delivery is capable of reducing DEN infection in cells and may be useful in decreasing DEN replication in humans. 相似文献
18.
19.
D.?P.?Barik S.?K.?Naik U.?Mohapatra P.?K.?ChandEmail author 《In vitro cellular & developmental biology. Plant》2004,40(5):467-470
Summary Multiple shoots were induced from cotyledonary nodes of grasspea (Lathyrus sativus L.) derived from 7-d-old in vitro seedlings on Murashige and Skoog (MS) medium containing N6-benzyladenine (BA), kinetin, or thidiazuron, BA being the most effective. Among the five genotypes tested, shoot proliferation
frequency was the highest (93.3%) for IC-120487, giving the maximum number of shoots (11.3 shoots per explant) on MS medium
augmented with 2.0 mgl−1 (8.87 μM) BA. Shoot cultures were established by repeatedly subculturing the original cotyledonary nodes on fresh medium after each
harvest of the newly formed shoots. Thus 30–40 shoots were obtained in 2 mo. from a single cotyledonary node. Up to 81.8%
of the shoots developed roots following transfer to half-strength MS medium containing 0.5 mgl−1 (2.85 μM) indole-3-acetic acid. Plantlets were successfully acclimatized and established in soil. 相似文献
20.
Roles of transition nuclear proteins in spermiogenesis 总被引:13,自引:0,他引:13
The transition nuclear proteins (TPs) constitute 90% of the chromatin basic proteins during the steps of spermiogenesis between histone removal and the deposition of the protamines. We first summarize the properties of the two major transition nuclear proteins, TP1 and TP2, and present concepts, based on their time of appearance in vivo and in vitro properties, regarding their roles. Distinct roles for the two TPs in histone displacement, sperm nuclear shaping, chromatin condensation, and maintenance of DNA integrity have been proposed. More definitive information on their roles in spermiogenesis has recently been obtained using mice with null mutations in the Tnp1 or Tnp2 genes for TP1 and TP2, respectively. In these mice, histone displacement and sperm nuclear shaping appear to progress quite normally. Spermatid nuclear condensation occurs, albeit in an abnormal fashion, and the mature sperm of the Tnp -null mutants are not as condensed as wild-type sperm. There is also evidence that sperm from these mutant mice contain an elevated level of DNA strand breaks. The mutant sperm showed several unexpected phenotypes, including a high incidence of configurational defects, such as heads bent back on midpieces, midpieces in hairpin configurations, coils, and clumps, other midpiece defects, reduced levels of proteolytic processing of protamine 2 during maturation, and reduced motility. The two TPs appear partly to compensate for each other as both Tnp1 - and Tnp2 -null mice were able to produce offspring, and appear to have largely overlapping functions as the two mutants had similar phenotypes. 相似文献