全文获取类型
收费全文 | 2091篇 |
免费 | 97篇 |
国内免费 | 3篇 |
专业分类
2191篇 |
出版年
2023年 | 13篇 |
2022年 | 24篇 |
2021年 | 46篇 |
2020年 | 36篇 |
2019年 | 39篇 |
2018年 | 46篇 |
2017年 | 28篇 |
2016年 | 62篇 |
2015年 | 86篇 |
2014年 | 93篇 |
2013年 | 140篇 |
2012年 | 141篇 |
2011年 | 177篇 |
2010年 | 89篇 |
2009年 | 79篇 |
2008年 | 104篇 |
2007年 | 105篇 |
2006年 | 106篇 |
2005年 | 100篇 |
2004年 | 78篇 |
2003年 | 72篇 |
2002年 | 71篇 |
2001年 | 35篇 |
2000年 | 31篇 |
1999年 | 34篇 |
1998年 | 17篇 |
1997年 | 14篇 |
1996年 | 12篇 |
1995年 | 17篇 |
1994年 | 11篇 |
1993年 | 15篇 |
1992年 | 26篇 |
1991年 | 20篇 |
1990年 | 17篇 |
1989年 | 20篇 |
1988年 | 23篇 |
1987年 | 15篇 |
1986年 | 14篇 |
1985年 | 17篇 |
1984年 | 11篇 |
1983年 | 10篇 |
1982年 | 11篇 |
1981年 | 10篇 |
1980年 | 10篇 |
1979年 | 8篇 |
1978年 | 8篇 |
1977年 | 8篇 |
1976年 | 6篇 |
1974年 | 9篇 |
1967年 | 5篇 |
排序方式: 共有2191条查询结果,搜索用时 15 毫秒
41.
42.
Bikram Pandey Janak R. Khatiwada Lin Zhang Kaiwen Pan Mohammed A. Dakhil Qinli Xiong Ram Kailash P. Yadav Mohan Siwakoti Akash Tariq Olusanya Abiodun Olatunji Meta Francis Justine Xiaogang Wu Xiaoming Sun Ziyan Liao Zebene Tadesse Negesse 《Ecology and evolution》2020,10(17):9474-9485
Studying the pattern of species richness is crucial in understanding the diversity and distribution of organisms in the earth. Climate and human influences are the major driving factors that directly influence the large‐scale distributions of plant species, including gymnosperms. Understanding how gymnosperms respond to climate, topography, and human‐induced changes is useful in predicting the impacts of global change. Here, we attempt to evaluate how climatic and human‐induced processes could affect the spatial richness patterns of gymnosperms in China. Initially, we divided a map of the country into grid cells of 50 × 50 km2 spatial resolution and plotted the geographical coordinate distribution occurrence of 236 native gymnosperm taxa. The gymnosperm taxa were separated into three response variables: (a) all species, (b) endemic species, and (c) nonendemic species, based on their distribution. The species richness patterns of these response variables to four predictor sets were also evaluated: (a) energy–water, (b) climatic seasonality, (c) habitat heterogeneity, and (d) human influences. We performed generalized linear models (GLMs) and variation partitioning analyses to determine the effect of predictors on spatial richness patterns. The results showed that the distribution pattern of species richness was highest in the southwestern mountainous area and Taiwan in China. We found a significant relationship between the predictor variable set and species richness pattern. Further, our findings provide evidence that climatic seasonality is the most important factor in explaining distinct fractions of variations in the species richness patterns of all studied response variables. Moreover, it was found that energy–water was the best predictor set to determine the richness pattern of all species and endemic species, while habitat heterogeneity has a better influence on nonendemic species. Therefore, we conclude that with the current climate fluctuations as a result of climate change and increasing human activities, gymnosperms might face a high risk of extinction. 相似文献
43.
Zhang K Aruva MR Shanthly N Cardi CA Patel CA Rattan S Cesarone G Wickstrom E Thakur ML 《Regulatory peptides》2007,144(1-3):91-100
Vasoactive intestinal peptide and pituitary adenylate cyclase activating peptide have high affinity for VPAC1, VPAC2 and PAC1 receptors overexpressed on human cancer cells. Four potent analogues of these peptides, TP3939, TP3982, TP4200 and TP3805 were labeled with (64)Cu and evaluated ex vivo and in vivo to asses their biological activity and receptor specificity. The ultimate goal is to utilize (64)Cu analogues for positron emission tomography (PET) imaging of breast cancers in humans. Radiochemical purity of each analogue was >92%. The muscle relaxivity assay revealed IC(50) to be 5.3x10(-8) M, 4.4x10(-8) M, 8.1x10(-8) M, 8.1x10(-9) M and Kd values determined by receptor specific cell binding assays were 3.3 nM, 0.33 nM, 0.2 nM and 0.72 nM for TP3805, TP3939, TP3982, and TP4200 respectively. The receptor affinity, using human breast cancer tissues, was 10.93 times greater than normal breast tissues. RT-PCR confirmed increased VPAC1 receptor expression on human breast tumor cells over normal cells and corroborated with autoradiography data. The blood clearance was rapid and in vivo translocation of (64)Cu to plasma protein was <15%. Data demonstrate that these analogues are potent, have uncompromised biological activity and are worthy of further evaluation for accurate PET imaging of human breast cancers and in determining malignant and benign lesions. 相似文献
44.
45.
46.
Raghavan Srividhya Malayaperumal Sarubala Mohan Viswanathan Balasubramanyam Muthuswamy 《Molecular and cellular biochemistry》2021,476(1):457-469
Molecular and Cellular Biochemistry - β-cell dysfunction is a critical determinant for both type 1 diabetes and type 2 diabetes and β-cells are shown to be highly susceptible to cellular... 相似文献
47.
Arunachalam M Mohan N Sugadev R Chellappan P Mahadevan A 《Biochimica et biophysica acta》2003,1621(3):261-265
Acinetobacter calcoaceticus MTC 127 was able to grow on catechin and protocatechuic acid (PCA) as sole carbon source. Cells induced with catechin oxidized catechin and PCA at rates higher than cells of uninduced cultures. Two aromatic compounds, PCA and phloroglucinol carboxylic acid (PGCA) were isolated from culture filtrate of cells grown in catechin and characterized by infrared spectrometry and high performance thin-layer chromatography. Moreover, A. calcoaceticus MTC 127 produced high levels of PCA compared to PGCA in the degradation of catechin. Based upon these results, a pathway for the degradation of (+)-catechin in A. calcoaceticus MTC 127 is proposed. Enzymes extracted from catechin-induced culture showed catechin oxygenase (cox) and protocatechuate 3,4-dioxygenase (pcd) activities. Catechin oxygenase was purified by column chromatography and SDS-PAGE analysis showed a single band with an apparent molecular weight of 47 kDa. 相似文献
48.
Ch. Mohan Rao B. Raman T. Ramakrishna K. Rajaraman D. Ghosh S. Datta V. D. Trivedi M. B. Sukhaswami 《International journal of biological macromolecules》1998,22(3-4)
α-Crystallin is a multimeric lenticular protein that has recently been shown to be expressed in several non-lenticular tissues as well. It is shown to prevent aggregation of non-native proteins as a molecular chaperone. By using a non-thermal aggregation model, we could show that this process is temperature-dependent. We investigated the chaperone-like activity of α-crystallin towards photo-induced aggregation of γ-crystallin, aggregation of insulin and on the refolding induced aggregation of β- and γ-crystallins. We observed that α-crystallin could prevent photo-aggregation of γ-crystallin and this chaperone-like activity of α-crystallin is enhanced several fold at temperatures above 30°C. This enhancement parallels the exposure of its hydrophobic surfaces as a function of temperature, probed using hydrophobic fluorescent probes such as pyrene and 8-anilinonaphthalene-1-sulfonate. We, therefore, concluded that α-crystallin prevents the aggregation of other proteins by providing appropriately placed hydrophobic surfaces; a structural transition above 30°C involving enhanced or re-organized hydrophobic surfaces of α-crystallin is important for its chaperone-like activity. We also addressed the issue of conformational aspects of target proteins and found that their aggregation prone molten globule states bind to α-crystallin. We trace these developments and discuss some new lines that suggest the role of tertiary structural aspects in the chaperone process. 相似文献
49.
Summary Relative efficiency of five chemical extractants for the extraction of available Zn in four different soils and its uptake by rice seedlings was studied in a pot culture experiment. The Zn extracted by dithizone-ammonium acetate showed a significant relationship with plant uptake whereas the values for other extractants except NH4OAc (pH4.8) did not approach the level of significance. Among the soil properties studied, pH and CaCO3 correlated negatively and organic matter and CEC positively with Zn uptake by rice plants.Contribution from the Soil Science and Agricultural Chemistry Dept., Banaras Hindu University, Varanasi-221005, India.Lecturer and Research Scholar of the Soil Science and Agricultural Chemistry Department, respectively.Lecturer and Research Scholar of the Soil Science and Agricultural Chemistry Department, respectively. 相似文献
50.
Influence of recirculation on the performance of anaerobic sequencing batch biofilm reactor (AnSBBR) was studied in the process of treating hypersaline (total dissolved inorganic solids (TDIS) approximately 26 g/l) and low biodegradable (BOD/COD approximately 0.3) composite chemical wastewater. Significant enhancement in the substrate removal efficiency and biogas yield was observed after introducing the recirculation to the system. Maximum efficiency (COD removal efficiency - 51%; SDR - 3.14 kg COD/cum-day) was observed at recirculation to feed (R/F) ratio of 2 (OLR - 6.15 kg C OD/cum-day; HLR - 2.30 cum (liquid)/cum day; UFV(A) - 0.023 m/h). Subsequent increase of R/F to 3 (OLR - 6.15 kg COD/cum-day; HLR - 3.07cum (liquid)/cum-day; UFV(A) - 0.035 m/h) resulted in reduction in COD removal efficiency (32%; SDR - 1.97 kg COD/cum-day). The enhanced performance of the system due to the introduction of recirculation was attributed to the improvement in the mass transfer between the substrate present in the bulk liquid and the attached biofilm. The hydrodynamic behavior due to recirculation mode of operation reduced the concentration gradient (substrate inhibition) of substrate and reaction by-products (VFA) resulting in mixed flow conditions. 相似文献