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81.
Current Status and Perspectives Regarding LNA‐Anti‐miR Oligonucleotides and microRNA miR‐21 Inhibitors as a Potential Therapeutic Option in Treatment of Colorectal Cancer 下载免费PDF全文
82.
Design and implementation of microarray gene expression markup language (MAGE-ML) 总被引:4,自引:0,他引:4 下载免费PDF全文
Spellman PT Miller M Stewart J Troup C Sarkans U Chervitz S Bernhart D Sherlock G Ball C Lepage M Swiatek M Marks WL Goncalves J Markel S Iordan D Shojatalab M Pizarro A White J Hubley R Deutsch E Senger M Aronow BJ Robinson A Bassett D Stoeckert CJ Brazma A 《Genome biology》2002,3(9):research0046.1-research00469
Background
Meaningful exchange of microarray data is currently difficult because it is rare that published data provide sufficient information depth or are even in the same format from one publication to another. Only when data can be easily exchanged will the entire biological community be able to derive the full benefit from such microarray studies.Results
To this end we have developed three key ingredients towards standardizing the storage and exchange of microarray data. First, we have created a minimal information for the annotation of a microarray experiment (MIAME)-compliant conceptualization of microarray experiments modeled using the unified modeling language (UML) named MAGE-OM (microarray gene expression object model). Second, we have translated MAGE-OM into an XML-based data format, MAGE-ML, to facilitate the exchange of data. Third, some of us are now using MAGE (or its progenitors) in data production settings. Finally, we have developed a freely available software tool kit (MAGE-STK) that eases the integration of MAGE-ML into end users' systems.Conclusions
MAGE will help microarray data producers and users to exchange information by providing a common platform for data exchange, and MAGE-STK will make the adoption of MAGE easier. 相似文献83.
Benjamin Bleyhl Marine Arakelyan Elshad Askerov Hendrik Bluhm Alexander Gavashelishvili Mamikon Ghasabian Arash Ghoddousi Aurel Heidelberg Igor Khorozyan Alexander Malkhasyan Karen Manvelyan Mohammadreza Masoud Ehsan M. Moqanaki Volker C. Radeloff Mahmood Soofi Paul Weinberg Nugzar Zazanashvili Tobias Kuemmerle 《Diversity & distributions》2019,25(1):129-141
84.
Alireza Kondori Mohammadreza Esmaeilirad Artem Baskin Boao Song Jialiang Wei Wei Chen Carlo U. Segre Reza Shahbazian‐Yassar David Prendergast Mohammad Asadi 《Liver Transplantation》2019,9(22)
Solid‐state electrocatalysis plays a crucial role in the development of renewable energy to reshape current and future energy needs. However, finding an inexpensive and highly active catalyst to replace precious metals remains a big challenge for this technology. Here, tri‐molybdenum phosphide (Mo3P) is found as a promising nonprecious metal and earth‐abundant candidate with outstanding catalytic properties that can be used for electrocatalytic processes. The catalytic performance of Mo3P nanoparticles is tested in the hydrogen evolution reaction (HER). The results indicate an onset potential of as low as 21 mV, H2 formation rate, and exchange current density of 214.7 µmol s?1 g?1cat (at only 100 mV overpotential) and 279.07 µA cm?2, respectively, which are among the closest values yet observed to platinum. Combined atomic‐scale characterizations and computational studies confirm that high density of molybdenum (Mo) active sites at the surface with superior intrinsic electronic properties are mainly responsible for the remarkable HER performance. The density functional theory calculation results also confirm that the exceptional performance of Mo3P is due to neutral Gibbs free energy (ΔGH*) of the hydrogen (H) adsorption at above 1/2 monolayer (ML) coverage of the (110) surface, exceeding the performance of existing non‐noble metal catalysts for HER. 相似文献
85.
Christian Erbel Grigorios Korosoglou Pearlyn Ler Mohammadreza Akhavanpoor Gabriele Domschke Fabian Linden Andreas O. Doesch Sebastian J. Buss Evangelos Giannitsis Hugo A. Katus Christian A. Gleissner 《PloS one》2015,10(11)
Background
CXCL4 is a platelet chemokine released at micromolar concentrations upon platelet activation. CXCL4 has been shown to promote atherogenesis by various mechanisms. However, data on CXCL4 plasma levels in patients with coronary artery disease are largely inconclusive. Computed coronary artery angiography (CCTA) represents an excellent tool to quantify and characterize coronary atherosclerotic plaques. We hypothesized that increased CXCL4 plasma levels may be associated with features of plaque instability resulting in adverse cardiovascular events. Specifically, we sought to determine whether CXCL4 levels are correlated with specific features of coronary artery disease including (1) plaque volume, (2) calcium score, (3) degree of stenosis, or (4) vascular remodeling.Methods and Results
CXCL4 plasma levels were measured by ELISA in 217 patients undergoing CCTA for suspected CAD (mean age 64.2 ± 9.4 years, 107 (49.3%) male). Mean CXCL4 plasma levels were 12.5 ± 4.6 ng/mL. There was no significant correlation between CXCL4 levels and any clinical or demographic parameters including cardiovascular risk factors. CXCL4 plasma levels did not differ between patient with or without coronary artery disease (CAD: 12.5 ± 4.5 ng/ml, no CAD: 12.5 ± 4.8 ng/ml). Neither univariate nor multivariate analysis showed an association between CXCL4 levels and plaque volume, total calcium score, degree of stenosis, or vascular remodeling. Subgroup analysis of patients with CAD as confirmed by CCTA did not show any association of CXCL4 levels with the extent of CAD.Conclusions
While CXCL4 may be present and active within the arterial wall, local increase of CXCL4 may not translate into systemically elevated CXCL4 levels. Further studies will have to test whether CXCL4 may still represent a suitable therapeutic target in human atherosclerosis. 相似文献86.
Azita Hekmatdoost Farhad Vahid Zahra Yari Mohammadreza Sadeghi Hassan Eini-Zinab Niknam Lakpour Soheila Arefi 《PloS one》2015,10(12)
Purpose
To determine whether 5-methylenetetrahydrofolate (MTHF) is more effective than folic acid supplementation in treatment of recurrent abortion in different MTHFR gene C677T and A1298C polymorphisms.Methods
A randomized, double blind, placebo-controlled trial conducted April 2011-September 2014 in recurrent abortion clinics in Tehran, Iran. The participants were women with three or more idiopathic recurrent abortion, aged 20 to 45 years. Two hundred and twenty eligible women who consented to participate were randomly assigned to receive either folic acid or 5-MTHF according to the stratified blocked randomization by age and the number of previous abortions. Participants took daily 1 mg 5-methylentetrahydrofolate or 1 mg folic acid from at least 8 weeks before conception to the 20th week of the pregnancy. The primary outcome was ongoing pregnancy rate at 20th week of pregnancy, and the secondary outcomes were serum folate and homocysteine at the baseline, after 8 weeks, and at the gestational age of 4, 8, 12, and 20 weeks, MTHFR gene C677T and A1298C polymorphisms.Results
There was no significant difference in abortion rate between two groups. Serum folate increased significantly in both groups over time; these changes were significantly higher in the group receiving 5-MTHF than the group receiving folic acid (value = 2.39, p<00.1) and the result was the same by considering the time (value = 1.24, p<0.01). Plasma tHcys decreased significantly in both groups over time; however these changes were not significantly different between the groups (value = 0.01, p = 0.47).Conclusion
The results do not support any beneficial effect of 5-MTHF vs. folate supplementation in women with recurrent abortion with any MTHFR C677T and/or A1298C polymorphism.Trial Registration
ClinicalTrials.gov NCT01976676相似文献87.
Light-directed synthesis of peptide nucleic acids (PNAs) chips 总被引:1,自引:0,他引:1
Liu ZC Shin DS Shokouhimehr M Lee KN Yoo BW Kim YK Lee YS 《Biosensors & bioelectronics》2007,22(12):2891-2897
We report herein the light-directed synthesis of peptide nucleic acids (PNAs) microarray using PNA monomers protected by photolabile protecting groups and a maskless technique that uses a digital micromirror array system to form virtual masks. An ultraviolet image from the virtual mask was cast onto the active surface of a glass substrate, which was mounted in a flow cell reaction chamber connected to a peptide synthesizer. Light exposure was followed by automatic chemical coupling cycles and these steps were repeated with different virtual masks to grow the desired PNA probes in a selected pattern. In a preliminary experiment, an array of PNA probes with dimensions of 4.11 mm × 4.11 mm was generated on each slide. Each synthesis region in the final array measured 210 μm × 210 μm for a total of 256 sites. The center-to-center space was 260 μm. It was observed from the hybridization pattern of the fluorescently labeled oligonucleotide targets that the fluorescence intensities of the matched, and mismatched sequences showed substantial difference, demonstrating specificity in the identification of complementary sequences. This opens the way to exploit processes from the microelectronics industry for the fabrication of PNA microarrays with high densities. 相似文献
88.
There is relatively little known about the demand for allograft tissues in Canada. The Canadian Council for Donation and Transplantation
(CCDT) is a national advisory body that undertook a comprehensive “market survey” to estimate surgical demand for human allograft
tissues in Canada. The report “Demand for Human Allograft Tissue in Canada” reflects survey results sent to 5 prominent User
Groups. User Groups were identified as orthopaedic surgeons; neurosurgeons; corneal transplant surgeons; plastic surgeons,
specifically those at Canadian Burn Units; and cardiac surgeons (adult and paediatric surgery). The demand for allograft grafts
was determined and then extrapolated across the total User Group and then increases in allograft tissue use over the next
1–2 years across User Groups were predicted. The overall response rate for the survey was 21.4%. It varied from a low of 19.6%
for the orthopaedic survey to a high of 40.5% for the corneal survey. The estimated current demand for allograft tissue in
Canada ranges from a low of 34,442 grafts per year to a high of 62,098 grafts per year. The predicted increase in use of allograft
tissue over the next 1–2 year period would suggest that annual demand could rise to somewhere in the range of 42,589–72,210
grafts. The highest rated preferences (98% and 94%) were for accredited and Canadian tissue banks, respectively. This study
represents a key step in addressing the paucity of information concerning the demand for allograft tissue in Canada. 相似文献
89.
Hamoun Anbari Amir Maghsoudi Mohammadreza Hosseinpour Fatemeh Yazdian 《Engineering in Life Science》2022,22(2):58
Curcumin is a polyphenolic molecule with antibacterial, antioxidant, anti‐inflammatory, and antimicrobial properties. This study aimed to prepare nanocurcumin by encapsulating in biopolymers to improve its stability, bioavailability, water‐solubility, antibacterial efficiency against methicillin‐resistant Staphylococcus aureus. Three effective variables of curcumin concentration, polymer concentration, and water volume on curcumin‐loaded polymer nanoparticles, were optimized. The average size of polyacrylic acid (PAA), polyvinyl alcohol (PVA), and polyethyleneimine (PEI) nanoparticles were obtained 75.2, 77.1, 86.4 nm, respectively. The nanoparticles had a spherical shape, a smooth and uniform surface morphology. The MIC of PAA, PVA, and PEI nanoparticles was 0.480, 0.390, and 0.340 mg/mL, respectively and the MIC of PAA, PVA, and PEI combined with methicillin was 0.330, 0.260, and 0.200 mg/mL, respectively. According to the results, curcumin‐loaded PEI nanoparticles had the highest inhibitory effect against methicillin‐resistant S. aureus among the synthesized nanoparticles. The results showed that solvent volume, polymer concentration and curcumin concentration had a significant effect on particle size. The inhibitory properties of curcumin nanoparticles significantly increased due to the smaller particle size and increased penetration into the bacterium. Curcumin‐loaded nanoparticles can be promising drug carriers for the treatment of infections, cancer, and other diseases. 相似文献
90.
Moniripiri Mohammad Hassani Soukht Abandani Mohammadreza Firoozabadi Bahar 《Biomechanics and modeling in mechanobiology》2023,22(2):711-727
Biomechanics and Modeling in Mechanobiology - Atherosclerosis, due to the permeation of low-density lipoprotein (LDL) particles into the arterial wall, is one of the most common and deadly diseases... 相似文献