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31.
Eswari J. Satya Majdoubi Jihen Naik Sweta Gupta Sneha Bit Arindam Rahimi-Gorji Mohammad Saleem Anber 《Biomechanics and modeling in mechanobiology》2020,19(5):1697-1711
Biomechanics and Modeling in Mechanobiology - Blood flow analysis in the artery is a paramount study in the field of arterial stenosis evaluation. Studies conducted so far have reported the... 相似文献
32.
33.
Pegah Rahimizadeh Tohid Rezaei Topraggaleh Mohammad Hossein Nasr‐Esfahani Niloofar Ziarati Shahab Mirshahvaladi Vahid Esmaeili Soroush Seifi Poopak Eftekhari‐Yazdi Abdolhossein Shahverdi 《Molecular reproduction and development》2020,87(1):115-123
Failed oocyte activation has been observed in unexplained infertile (UI) and asthenoteratozoospermic (AT) men. The deficiency of phospholipase C‐zeta (PLCζ) could be a possible reason for such failures and has not been studied yet. We investigated the expression and localization of PLCζ protein in the sperms of patients with UI and AT conditions. The relationships between PLCζ‐related parameters with male age, sperm characteristics, DNA integrity, and cellular maturity were assessed. Semen samples were collected from fertile (n = 40), UI (n = 40), and AT (n = 40) men. Subsequently, semen analysis, DNA fragmentation, hyaluronic acid‐binding ability, and PLCζ level along with its distribution were evaluated using computer‐assisted sperm analyzer, sperm chromatin structure assay (SCSA), hyaluronic acid‐binding assay (HBA), western blot analysis and immunofluorescence microscopy, respectively. Unlike SCSA, the values of HBA, and PLCζ expression were significantly reduced in UI and AT patients compared to fertile men, whereas no significant differences were observed among the experimental groups in terms of PLCζ localization patterns. The regression analysis also showed that HBA is the only variable associated with PLCζ levels. Furthermore, the correlation of male age with PLCζ localization in postacrosomal, equatorial, and acrosomal+postacrosomal+equatorial (A+PA+E) patterns, as well as the relation of normal morphology, with the (A+PA+E) pattern, remained in the regression model. Our findings indicated that reduced PLCζ level along with the increased DNA fragmentation and impaired maturation may be possible etiologies of decreased fertilization in the studied subjects. 相似文献
34.
Fatemeh Farshchi Mohammad Hasanzadeh Ahad Mokhtarzadeh 《Journal of molecular recognition : JMR》2020,33(4)
In this study, a novel electroconductive interface was prepared based on Fe3O4 magnetic nanoparticle and cysteamine functionalized gold nanoparticle. The engineered interface was used as signal amplification substrate in the electrochemical analysis of antibody‐antigen binding. For this purpose, biotinilated‐anti‐prostate‐specific antigen (PSA) antibody was bioconjugated with iron oxide magnetic nanoparticles (Fe3O4) and drop‐casted on the surface of glassy carbon electrode (GCE). Also, secondary antibody (HRP‐Ab2) encapsulated on gold nanoparticles caped by cysteamine was immobilized on the surface of GCE modified electrode. A transmission electron microscopy images shows that a sandwich immunoreaction was done and binding of Ab1 and Ab2 performed successfully. Various parameters of immunoassay, including the loading of magnetic nanoparticles, the amount of gold nanoparticle conjugate, and the immunoreaction time, were optimized. The detection limit of 0.001 μg. L?1 of PSA was obtained under optimum experimental conditions. It is found that such magneto‐bioassay could be readily used for simultaneous parallel detection of multiple proteins by using multiple inorganic metal nanoparticle tracers and are expected to open new opportunities for early stage diagnosis of cancer in near future. 相似文献
35.
Liping Liu Lihua Zhao Piaojuan Chen Hanyang Cai Zhimin Hou Xingyue Jin Mohammad Aslam Mengnan Chai Linyi Lai Qing He Yanhui Liu Xiaoyi Huang Huihuang Chen Yingzhi Chen Yuan Qin 《The Plant journal : for cell and molecular biology》2020,102(6):1172-1186
Angiosperm reproductive development is a complex event that includes floral organ development, male and female gametophyte formation and interaction between the male and female reproductive organs for successful fertilization. Previous studies have revealed the redundant function of ATP binding cassette subfamily G (ABCG) transporters ABCG1 and ABCG16 in pollen development, but whether they are involved in other reproductive processes is unknown. Here we show that ABCG1 and ABCG16 were not only expressed in anthers and stamen filaments but also enriched in pistil tissues, including the stigma, style, transmitting tract and ovule. We further demonstrated that pistil‐expressed ABCG1 and ABCG16 promoted rapid pollen tube growth through their effects on auxin distribution and auxin flow in the pistil. Moreover, disrupted auxin homeostasis in stamen filaments was associated with defective filament elongation. Our work reveals the key functions of ABCG1 and ABCG16 in reproductive development and provides clues for identifying ABCG1 and ABCG16 substrates in Arabidopsis. 相似文献
36.
Abbu Zaid Firoz Mohammad Qazi Fariduddin 《Physiology and Molecular Biology of Plants》2020,26(1):25-39
Menthol mint (Mentha arvensis L.) cultivation is significantly affected by the heavy metals like cadmium (Cd) which also imposes severe health hazards. Two menthol mint cultivars namely Kosi and Kushal were evaluated under Cd stress conditions. Impact of plant growth regulators (PGRs) like salicylic acid (SA), gibberellic acid (GA3) and triacontanol (Tria) on Cd stress tolerance was assessed. Reduced growth, photosynthetic parameters, mineral nutrient concentration, and increased oxidative stress biomarkers like electrolyte leakage, malondialdehyde, and hydrogen peroxide contents were observed under Cd stress. Differential upregulation of proline content and antioxidant activities under Cd stress was observed in both the cultivars. Interestingly, low electrolyte leakage, lipid peroxidation, hydrogen peroxide and Cd concentration in leaves were observed in Kushal compared to Kosi. Among all the PGRs tested, SA proved to be the best in improving Cd-stress tolerance in both the cultivars but Kushal responded better than Kosi. 相似文献
37.
Dastmalchi Narges Safaralizadeh Reza Hosseinpourfeizi Mohammad Ali Baradaran Behzad Khojasteh Seyed Mahdi Banan 《Molecular biology reports》2021,48(2):1345-1357
Molecular Biology Reports - Combination therapy has been considered as a potential method to overcome the BC chemoresistance. MicroRNAs (miRs) have been suggested as a therapeutic factor in the... 相似文献
38.
Nemati Sara Pazoki Hossein Mohammad Rahimi Hanieh Asadzadeh Aghdaei Hamid Shahrokh Shabnam Baghaei Kaveh Mirjalali Hamed Zali Mohammad Reza 《Molecular biology reports》2021,48(10):7041-7047
Molecular Biology Reports - Autophagy process is an important defense mechanism against intracellular infection. This process plays a critical role in limiting the development of Toxoplasma gondii.... 相似文献
39.
Saremi Leila Shafizadeh Marziyeh Esmaeilzadeh Emran Ghaffari Mohammad Ebrahim Mahdavi Mohammad hosein Amid Reza Kadkhodazadeh Mahdi 《Molecular biology reports》2021,48(3):2285-2290
Molecular Biology Reports - Peri-implantitis (PI) is a multifactorial condition caused by the interactions of pathogens and the host immune response. Previous studies have demonstrated a... 相似文献
40.
Mirbolook Atena Rasouli-Sadaghiani MirHassan Sepehr Ebrahim Lakzian Amir Hakimi Mohammad 《Journal of Plant Growth Regulation》2021,40(2):831-847
Journal of Plant Growth Regulation - Plants require optimum amounts of nutrients for suitable growth and yield production. Accordingly, the most efficient methods of fertilization, including the... 相似文献