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211.
Mansouri Borhan Błaszczyk Martyna Binkowski Lukasz J. Sayadi Mohammad Hossein Azadi Nammam Ali Amirabadizadeh Ali Reza Mehrpour Omid 《Biological trace element research》2020,195(1):63-70
Biological Trace Element Research - In low-income and middle-income countries such as Iran, smoking is becoming increasingly popular, especially among young people. This has led to additional... 相似文献
212.
Muhammad S. Nadeem Mohammed Razeeth Hani M. Z. Choudhry Firoz Anwar Mazin A. Zamzami Bibi N. Murtaza Fahad A. M. Al-Abbasi Mohammad I. Khan Abdul R. Shakoori 《Journal of cellular biochemistry》2020,121(1):125-134
Escherichia coli is frequently exploited for genetic manipulations and heterologous gene expression studies. We have evaluated the metabolic profile of E. coli strain BL21 (DE3) RIL CodonPlus after genetic modifications and subjecting to the production of recombinant protein. Three genetically variable E. coli cell types were studied, normal cells (susceptible to antibiotics) cultured in simple LB medium, cells harboring ampicillin-resistant plasmid pET21a (+), grown under antibiotic stress, and cells having recombinant plasmid pET21a (+) ligated with bacterial lactate dehydrogenase gene grown under ampicillin and standard isopropyl thiogalactoside (IPTG)-induced gene expression conditions. A total of 592 metabolites were identified through liquid chromatography-mass spectrometry/mass spectrometry analysis, feature and peak detection using XCMS and CAMERA followed by precursor identification by METLIN-based procedures. Overall, 107 metabolites were found differentially regulated among genetically modified cells. Quantitative analysis has shown a significant modulation in DHNA-CoA, p-aminobenzoic acid, and citrulline levels, indicating an alteration in vitamin K, folic acid biosynthesis, and urea cycle of E. coli cells during heterologous gene expression. Modulations in energy metabolites including NADH, AMP, ADP, ATP, carbohydrate, terpenoids, fatty acid metabolites, diadenosine tetraphosphate (Ap4A), and l -carnitine advocate major metabolic rearrangements. Our study provides a broader insight into the metabolic adaptations of bacterial cells during gene manipulation experiments that can be prolonged to improve the yield of heterologous gene products and concomitant production of valuable biomolecules. 相似文献
213.
Mohammad Foad Abazari Fatemeh Soleimanifar Seyed Ehsan Enderami Navid Nasiri Fatemeh Nejati Seyed Ahmad Mousavi Masoud Soleimani Jafar Kiani Pegah Ghoraeian Mousa Kehtari 《Journal of cellular biochemistry》2020,121(2):1169-1181
Human-induced pluripotent stem cells-derived hepatocyte-like cells (hiPSCs-HLCs) holds considerable promise for future clinical personalized therapy of liver disease. However, the low engraftment of these cells in the damaged liver microenvironment is still an obstacle for potential application. In this study, we explored the effectiveness of decellularized amniotic membrane (dAM) matrices for culturing of iPSCs and promoting their differentiation into HLCs. The DNA content assay and histological evaluation indicated that cellular and nuclear residues were efficiently eliminated and the AM extracellular matrix component was maintained during decelluarization. DAM matrices were developed as three-dimensional scaffolds and hiPSCs were seeded into these scaffolds in defined induction media. In dAM scaffolds, hiPSCs-HLCs gradually took a typical shape of hepatocytes (polygonal morphology). HiPSCs-HLCs that were cultured into dAM scaffolds showed a higher level of hepatic markers than those cultured in tissue culture plates (TCPs). Moreover, functional activities in term of albumin and urea synthesis and CYP3A activity were significantly higher in dAM scaffolds than TCPs over the same differentiation period. Thus, based on our results, dAM scaffold might have a considerable potential in liver tissue engineering, because it can improve hepatic differentiation of hiPSCs which exhibited higher level of the hepatic marker and more stable metabolic functions. 相似文献
214.
215.
Javad Amini Mahabadi Abolfazl Aazami Tameh Sayyed Alireza Talaei Mohammad Karimian Tahereh Rahiminia Seyed Ehsan Enderami Seyed Mohammad Gheibi Hayat Hossein Nikzad 《Journal of cellular biochemistry》2020,121(3):2159-2169
Numerous reagents were employed for differentiating induced pluripotent stem cells (iPSCs) into male germ cells; however, the induction procedure was ineffective. The aim of this study was to improve the in vitro differentiation of mice iPSCs (miPSCs) into male germ cells with retinoic acid (RA) and progesterone (P). miPSCs were differentiated to embryoid bodies (EBs) in suspension with RA with or without progesterone for 0, 4, and 7 days. Then, the expression of certain genes at different stages of male germ cell development including Ddx4 (pre meiosis), Stra8 (meiosis), AKAP3 (post meiosis), and Mvh protein was examined in RNA and/or protein levels by real-time polymerase chain reaction or flow cytometry, respectively. The Stra8 gene expression increased in the RA groups on all days. But, expression of this gene declined in RA + P groups. In addition, an increased expression of Ddx4 gene was observed on day 0 in the P group. Also, a significant upregulation was observed in the expression of AKAP3 gene in the RA + P group on days 0 and 4. However, gene expression decreased in P and RA groups on day 7. The expression of Mvh protein significantly increased in the RA group on day 7. The Mvh expression was also enhanced in the P group on day 4, but it decreased on day 7, while this protein upregulated on day 0 and 7 in the RA + P group. The miPSCs have the capacity for in vitro differentiation into male germ cells by RA and/or progesterone. However, the effects of these inducers depend on the type of combination and an effective time. 相似文献
216.
Mohd Amir Taj Mohammad Kartikay Prasad Gulam Mustafa Hasan Vijay Kumar Ravins Dohare 《Journal of biomolecular structure & dynamics》2020,38(15):4625-4634
Communicated by Ramaswamy H. Sarma 相似文献
217.
Zahra Khoshbin Mohammad Izadyar Mohammad Reza Bozorgmehr Asma Verdian 《Journal of biomolecular structure & dynamics》2020,38(12):3659-3675
AbstractThe aptamers with the ability to form a G-quadruplex structure can be stable in the presence of some ions. Hence, study of the interactions between such aptamers and ions can be beneficial to determine the highest selective aptamer toward an ion. In this article, molecular dynamics (MD) simulations and quantum mechanics (QM) calculations have been applied to investigate the selectivity of the T30695 aptamer toward Pb2+ in comparison with some ions. The Free Energy Landscape (FEL) analysis indicates that Pb2+ has remained inside the aptamer during the MD simulation, while the other ions have left it. The Molecular Mechanics Poisson-Boltzmann Surface Area (MM-PBSA) binding energies prove that the conformational stability of the aptamer is the highest in the presence of Pb2+. According to the compaction parameters, the greatest compressed ion-aptamer complex, and hence, the highest ion-aptamer interaction have been induced in the presence of Pb2+. The contact maps clarify the closer contacts between the nucleotides of the aptamer in the presence of Pb2+. The density functional theory (DFT) results show that Pb2+ forms the most stable complex with the aptamer, which is consistent with the MD results. The QM calculations reveal that the N-H bonds and the O…H distances are the longest and the shortest, respectively, in the presence of Pb2+. The obtained results verify that the strongest hydrogen bonds (HBs), and hence, the most compressed aptamer structure are induced by Pb2+. Besides, atoms in molecules (AIM) and natural bond orbital (NBO) analyses confirm the results.Communicated by Ramaswamy H. Sarma 相似文献
218.
Ghazizadeh Hamideh Rezaei Majid Avan Amir Fazilati Mohammad Pasdar Alireza Tavallaie Shima Kazemi Elham Seyedi Seyed Mohammad Reza Ferns Gordon A. Azimi-Nezhad Mohsen Ghayour-Mobarhan Majid 《Molecular biology reports》2020,47(2):867-875
Molecular Biology Reports - Metabolic syndrome (MetS) is associated with a pro-inflammatory state and endothelial dysfunction that places subjects with MetS at a higher risk of atherosclerosis.... 相似文献
219.
Roshankhah Shiva Abdolmaleki Amir Salahshoor Mohammad Reza 《Molecular biology reports》2020,47(8):6053-6065
Molecular Biology Reports - Mercuric chloride (MC) is a complex substance which is capable to produce free radicals. Middle Eastern Phoenix dactylifera (MEPD) is a flowering plant of palm family... 相似文献
220.
Najafzadeh Mohammad Javad Dolatabadi Somayeh de Hoog Sybren Esfahani Mahmoud Karimizadeh Haghani Iman Aghili Seyed Reza Ghazvini Roshanak Daei Rezaei-Matehkolaei Ali Abastabar Mahdi Al-Hatmi Abdullah M. S. 《Mycopathologia》2020,185(3):515-525
Mycopathologia - Fungi of the genus Fusarium are well known as major plant pathogens but also cause a broad spectrum of human infections. Sixty-three clinical isolates, collected during... 相似文献