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排序方式: 共有209条查询结果,搜索用时 8 毫秒
61.
Lise Comte Juan Carvajal‐Quintero Pablo A. Tedesco Xingli Giam Ulrich Brose Tibor Ers Ana F. Filipe Marie‐Jose Fortin Katie Irving Claire Jacquet Stefano Larsen Sapna Sharma Albert Ruhi Fernando G. Becker Lilian Casatti Giuseppe Castaldelli Renato B. Dala‐Corte Stephen R. Davenport Nathan R. Franssen Emili García‐Berthou Anna Gavioli Keith B. Gido Luz Jimenez‐Segura Rafael P. Leito Bill McLarney Jason Meador Marco Milardi David B. Moffatt Thiago V. T. Occhi Paulo S. Pompeu David L. Propst Mark Pyron Gilberto N. Salvador Jerome A. Stefferud Tapio Sutela Christopher Taylor Akira Terui Hirokazu Urabe Teppo Vehanen Jean R. S. Vitule Jaquelini O. Zeni Julian D. Olden 《Global Ecology and Biogeography》2021,30(1):38-50
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C. Gaillard B. A. Moffatt M. Blacker M. Laloue 《Molecular genetics and genomics : MGG》1998,257(3):348-353
Four mutants of Arabidopsis thaliana that are deficient in adenine phosphoribosyl transferase (APRT) activity have been isolated by selecting for germination of seeds and growth of the plantlets on a medium containing 2,6-diaminopurine (DAP), a toxic analog of adenine. In all mutants, DAP resistance is due to a recessive nuclear mutation at a locus designated apt. The mutants are male sterile due to pollen abortion after meiosis. Furthermore, it has been shown that metabolism of cytokinins is impaired in the mutant BM3, which has the lowest level of APRT activity among the mutants tested. However, three different cDNAs encoding APRT have been isolated in A. thaliana and this raised the question of the nature of the mutation which results in low APRT activity. The mutation was genetically mapped to chromosome I and lies within 6 cM of the phenotypic marker dis2, indicating that the mutation affects the APT1 gene, a result confirmed by sequencing of mutant alleles. The mutation in the allele apt1-3 is located at the 5′ splicing site of the third intron, and eliminates a BstNI restriction site, as verified by Southern blotting and PCR fragment length analysis. 相似文献
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The Arabidopsis HOMOLOGY-DEPENDENT GENE SILENCING1 gene codes for an S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing 下载免费PDF全文
Rocha PS Sheikh M Melchiorre R Fagard M Boutet S Loach R Moffatt B Wagner C Vaucheret H Furner I 《The Plant cell》2005,17(2):404-417
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Chernova TA Romanyuk AV Karpova TS Shanks JR Ali M Moffatt N Howie RL O'Dell A McNally JG Liebman SW Chernoff YO Wilkinson KD 《Molecular cell》2011,43(2):242-252
Yeast prions are self-perpetuating, QN-rich amyloids that control heritable traits and serve as a model for mammalian amyloidoses. De novo prion formation by overproduced prion protein is facilitated by other aggregated QN-rich protein(s) and is influenced by alterations of protein homeostasis. Here we explore the mechanism by which the Las17-binding protein Lsb2 (Pin3) promotes conversion of the translation termination factor Sup35 into its prion form, [PSI(+)]. We show that Lsb2 localizes with some Sup35 aggregates and that Lsb2 is a short-lived protein whose levels are controlled via the ubiquitin-proteasome system and are dramatically increased by stress. Loss of Lsb2 decreases stability of [PSI(+)] after brief heat shock. Mutations interfering with Lsb2 ubiquitination increase prion induction, while a mutation eliminating association of Lsb2 with the actin cytoskeleton blocks its aggregation and prion-inducing ability. These findings directly implicate the UPS and actin cytoskeleton in regulating prions via a stress-inducible QN-rich protein. 相似文献
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Rahman LN Smith GS Bamm VV Voyer-Grant JA Moffatt BA Dutcher JR Harauz G 《Biochemistry》2011,50(44):9587-9604
Group 2 late embryogenesis abundant (LEA) proteins, also known as dehydrins, are intrinsically disordered proteins that are expressed in plants experiencing extreme environmental conditions such as drought or low temperatures. These proteins are characterized by the presence of at least one conserved, lysine-rich K-segment and sometimes by one or more serine-rich S-segments that are phosphorylated. Dehydrins may stabilize proteins and membrane structures during environmental stress and can sequester and scavenge metal ions. Here, we investigate how the conformations of two dehydrins from Thellungiella salsuginea, denoted as TsDHN-1 (acidic) and TsDHN-2 (basic), are affected by pH, interactions with cations and membranes, and phosphorylation. Both TsDHN-1 and TsDHN-2 were expressed as SUMO fusion proteins for in vitro phosphorylation by casein kinase II (CKII), and structural analysis by circular dichroism and attenuated total reflection-Fourier transform infrared spectroscopy. We show that the polyproline II conformation can be induced in the dehydrins by their environmental conditions, including changes in the concentration of divalent cations such as Ca(2+). The assembly of actin by these dehydrins was assessed by sedimentation assays and viewed by transmission electron and atomic force microscopy. Phosphorylation allowed both dehydrins to polymerize actin filaments. These results support the hypothesis that dehydrins stabilize the cytoskeleton under stress conditions and further that phosphorylation may be an important feature of this stabilization. 相似文献
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The use of ghostwriters by industry is subject to increasing public attention and scrutiny. This article addresses the practice and ethics of scientific ghostwriting. We focus on the type of ghostwriting that involves a pharmaceutical company hiring a medical education and communications company to write a paper favorable of their product, who then hires a well-known academic to publish it under his or her name without disclosing the paper's true origins. We argue that this practice is harmful both to the public and to the institutions of science and that it is not justified by an analogy to accepted scientific authorship practices. Finally, we consider ways to discourage the practice. 相似文献