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E.A. SCHLÜNS B.J. WEGENER‡ H. SCHLÜNS N. AZUMA† S. K. A. ROBSON R. H. CROZIER 《Molecular ecology》2009,18(1):156-167
Weaver ants ( Oecophylla smaragdina ) are dominant ants in open forests from India, Australia, China and Southeast Asia, whose leaf nests are held together with larval silk. The species, together with its sole congener O. longinoda , has been important in research on biological control, communication, territoriality and colony integration. Over most of the range, only one queen has been found per colony, but the occurrence of several queens per nest has been reported for the Australian Northern Territory. The number of males mating with each queen is little known. Here we report on the colony structure of O. smaragdina using published and new microsatellite markers. Worker genotype arrays reflect the occurrence of habitual polygyny (more than one queen per colony) in 18 colonies from Darwin, Northern Australia, with up to five queens inferred per colony. Monogyny (one queen per colony) with occasional polygyny was inferred for 14 colonies from Queensland, Australia, and 20 colonies from Java, Indonesia. Direct genotyping of the sperm carried by 77 Queensland queens and worker genotypic arrays of established colonies yielded similar results, indicating that less than half of the queens mate only once and some mate up to five times. Worker genotype arrays indicated that queens from Java and the Northern Territory also often mate with more than one male, but less often than those from Queensland. A strong isolation-by-distance effect was found for Queensland samples. The variation uncovered means that O. smaragdina is a more versatile study system than previously supposed. 相似文献
104.
Genome-wide scan on total serum IgE levels identifies FCER1A as novel susceptibility locus
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Weidinger S Gieger C Rodriguez E Baurecht H Mempel M Klopp N Gohlke H Wagenpfeil S Ollert M Ring J Behrendt H Heinrich J Novak N Bieber T Krämer U Berdel D von Berg A Bauer CP Herbarth O Koletzko S Prokisch H Mehta D Meitinger T Depner M von Mutius E Liang L Moffatt M Cookson W Kabesch M Wichmann HE Illig T 《PLoS genetics》2008,4(8):e1000166
High levels of serum IgE are considered markers of parasite and helminth exposure. In addition, they are associated with allergic disorders, play a key role in anti-tumoral defence, and are crucial mediators of autoimmune diseases. Total IgE is a strongly heritable trait. In a genome-wide association study (GWAS), we tested 353,569 SNPs for association with serum IgE levels in 1,530 individuals from the population-based KORA S3/F3 study. Replication was performed in four independent population-based study samples (total n = 9,769 individuals). Functional variants in the gene encoding the alpha chain of the high affinity receptor for IgE (FCER1A) on chromosome 1q23 (rs2251746 and rs2427837) were strongly associated with total IgE levels in all cohorts with P values of 1.85 x 10(-20) and 7.08 x 10(-19) in a combined analysis, and in a post-hoc analysis showed additional associations with allergic sensitization (P = 7.78 x 10(-4) and P = 1.95 x 10(-3)). The "top" SNP significantly influenced the cell surface expression of FCER1A on basophils, and genome-wide expression profiles indicated an interesting novel regulatory mechanism of FCER1A expression via GATA-2. Polymorphisms within the RAD50 gene on chromosome 5q31 were consistently associated with IgE levels (P values 6.28 x 10(-7)-4.46 x 10(-8)) and increased the risk for atopic eczema and asthma. Furthermore, STAT6 was confirmed as susceptibility locus modulating IgE levels. In this first GWAS on total IgE FCER1A was identified and replicated as new susceptibility locus at which common genetic variation influences serum IgE levels. In addition, variants within the RAD50 gene might represent additional factors within cytokine gene cluster on chromosome 5q31, emphasizing the need for further investigations in this intriguing region. Our data furthermore confirm association of STAT6 variation with serum IgE levels. 相似文献
105.
Impacts of land use on riparian forest along an urban – rural gradient in southern Manitoba 总被引:3,自引:0,他引:3
Extensive landscape modification by humans has led to the fragmentation of riparian forests across North America. We compared
the vegetation of extant riparian forest along an urban-rural disturbance gradient. In 1999, twenty-five sites along Assiniboine
River in Manitoba, Canada were categorized according to land use: urban, suburban, high intensity rural, low intensity rural,
and relatively high quality reference forest. Differences in herbaceous, shrub, and tree species composition and diversity
were related to the proportion of surrounding land use, forest patch size, connectivity, and area:perimeter ratio. Urban riparian
forests were more disturbed and isolated. They were smaller and characterized by drier, more alkaline soils. Moreover, they
had significantly lower native and overall understorey species diversity, and had a higher proportion of exotics including
Solanum dulcamara and Hesperis matronalis. Suburban forests were less disturbed, faced greater development pressure, and had sandier soils. Although suburban understorey
diversity was similar to that of rural forests, suburban sites had a higher proportion of exotic species, especially escaped
horticultural and invasive species including Caragana arborescens and Rhamnus cathartica. Reference sites were relatively large and exhibited greater connectivity, but there was little difference in species composition
and diversity among high intensity rural, low intensity rural, and reference sites. These site types were less disturbed than
either urban or suburban forests, and reference sites were characterized by hydrophilic species including Scirpus fluviatilis and Carex aquatilis. Our results suggest that landscape measures of disturbance, and related changes in environment, may be confidently used
to assess impacts of land use on vegetation along urban-rural gradients.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
106.
Isolation and Characterization of Mutants Defective in Seed Coat Mucilage Secretory Cell Development in Arabidopsis 总被引:7,自引:2,他引:5
Tamara L. Western Joanne Burn Wei Ling Tan Debra J. Skinner Luke Martin-McCaffrey Barbara A. Moffatt George W. Haughn 《Plant physiology》2001,127(3):998-1011
In Arabidopsis, fertilization induces the epidermal cells of the outer ovule integument to differentiate into a specialized seed coat cell type producing extracellular pectinaceous mucilage and a volcano-shaped secondary cell wall. Differentiation involves a regulated series of cytological events including growth, cytoplasmic rearrangement, mucilage synthesis, and secondary cell wall production. We have tested the potential of Arabidopsis seed coat epidermal cells as a model system for the genetic analysis of these processes. A screen for mutants defective in seed mucilage identified five novel genes (MUCILAGE-MODIFIED [MUM]1–5). The seed coat development of these mutants, and that of three previously identified ones (TRANSPARENT TESTA GLABRA1, GLABRA2, and APETALA2) were characterized. Our results show that the genes identified define several events in seed coat differentiation. Although APETALA2 is needed for differentiation of both outer layers of the seed coat, TRANSPARENT TESTA GLABRA1, GLABRA2, and MUM4 are required for complete mucilage synthesis and cytoplasmic rearrangement. MUM3 and MUM5 may be involved in the regulation of mucilage composition, whereas MUM1 and MUM2 appear to play novel roles in post-synthesis cell wall modifications necessary for mucilage extrusion. 相似文献
107.
Osteoblasts and bone lining cells form a near continuous layer covering the bone surface and interactions between these cells and the organic matrix of bone are important determinants of osteoblast proliferation and differentiation. In addition, cells of the osteoblast-lineage form functional communications with each other, with the extra-cellular matrix and with osteocytes through cytoplasmic processes extending through canaliculi in the bone. Together, these cells form a network of putative importance in the regulation of skeletal homeostasis. Cell-cell and cell-matrix interactions are mediated by members of several families of cell adhesion molecules, and knowledge of their interactions will be of fundamental importance in understanding the role of osteoblast in skeletal turnover. Here, the expression pattern of members of the major families of cell adhesion molecules by cells of the osteoblast lineage is reviewed. Special emphasis has been placed on human tissues. In addition, the possibility that cells at progressive stages of the osteoblast lineage have different profiles of cell adhesion molecule expression is explored, and the putative significance of cell-matrix interactions in human skeletal disease briefly discussed. 相似文献
108.
Adenosine kinase deficiency is associated with developmental abnormalities and reduced transmethylation 总被引:12,自引:0,他引:12
Moffatt BA Stevens YY Allen MS Snider JD Pereira LA Todorova MI Summers PS Weretilnyk EA Martin-McCaffrey L Wagner C 《Plant physiology》2002,128(3):812-821
Adenosine (Ado) kinase (ADK; ATP:Ado 5' phosphotransferase, EC 2.7.1.20) catalyzes the salvage synthesis of adenine monophosphate from Ado and ATP. In Arabidopsis, ADK is encoded by two cDNAs that share 89% nucleotide identity and are constitutively, yet differentially, expressed in leaves, stems, roots, and flowers. To investigate the role of ADK in plant metabolism, lines deficient in this enzyme activity have been created by sense and antisense expression of the ADK1 cDNA. The levels of ADK activity in these lines range from 7% to 70% of the activity found in wild-type Arabidopsis. Transgenic plants with 50% or more of the wild-type activity have a normal morphology. In contrast, plants with less than 10% ADK activity are small with rounded, wavy leaves and a compact, bushy appearance. Because of the lack of elongation of the primary shoot, the siliques extend in a cluster from the rosette. Fertility is decreased because the stamen filaments do not elongate normally; hypocotyl and root elongation are reduced also. The hydrolysis of S-adenosyl-L-homo-cysteine (SAH) produced from S-adenosyl-L-methionine (SAM)-dependent methylation reactions is a key source of Ado in plants. The lack of Ado salvage in the ADK-deficient lines leads to an increase in the SAH level and results in the inhibition of SAM-dependent transmethylation. There is a direct correlation between ADK activity and the level of methylesterified pectin in seed mucilage, as monitored by staining with ruthenium red, immunofluorescence labeling, or direct assay. These results indicate that Ado must be steadily removed by ADK to prevent feedback inhibition of SAH hydrolase and maintain SAM utilization and recycling. 相似文献
109.
Application of a serum-free medium for the growth of Vero cells and the production of reovirus 总被引:3,自引:0,他引:3
Butler M Burgener A Patrick M Berry M Moffatt D Huzel N Barnabé N Coombs K 《Biotechnology progress》2000,16(5):854-858
Two strains of reovirus (serotype 1 Lang/TIL and serotype 3 Dearing/T3D) were propagated in Vero cells grown in stationary or agitated cultures in a serum-free medium, M-VSFM. Solid microcarriers (Cytodex-1) were used to support cell growth in agitated cultures with a normal doubling time of 25 h. Cell yields of 1 x 10(6) cells/mL were obtained from an inoculum of 2 x 10(5) cells/mL in 4 days in microcarrier cultures. The growth profile and cell yield was not significantly different from serum-supplemented cultures. The virus titer increased by 3-4 orders of magnitude over a culture period of 150 h. The maximum virus titer in stationary cultures reached >1 x 10(9) pfu/mL for both strains of reovirus in M-VSFM. M-VSFM also supported high viral yields in microcarrier cultures. Both the specific productivity and final viral yield was higher in M-VSFM than serum-supplemented cultures. The high viral productivity suggests that this is a suitable system for the production of reovirus as an oncolytic agent for human therapeutic use. 相似文献
110.