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61.
62.
The objective of this work was to study the role of the phytochromes (phy) B, D and E in the thermoperiodic control of elongation
and flowering time in Arabidopsis thaliana. WT, and phyB, phyD and phyE single mutants, and phyB phyD and phyB phyE double mutants, were grown under day/night temperatures (DT/NT) of 12/22°C, 17/17°C or 22/12°C (negative, zero and positive
DIF, respectively) for inflorescence stem length measurements, and under DT/NT 17/25°C or 25/17°C (negative and positive DIF,
respectively) for leaf morphology and flowering time measurements. In WT final length of the stem, petiole and leaf blade
were longer under positive DIF compared to negative DIF. The temperature effect was stronger in the leaf petiole than the
stem, whereas only a slight change was seen in the leaf blade length direction and none in the width direction. The temperature
effect on stem and petiole elongation was reduced or nearly eliminated in the genotypes lacking phyB, while a phyD or a phyE mutation had no influence or a slightly positive influence on the temperature effect, respectively. These results suggest
that phyB, and not phyD or phyE, is needed for a complete thermoperiodic control of elongation growth in A. thaliana. For all genotypes tested, plants flowered earlier at negative DIF than positive DIF, suggesting that none of the three phytochromes
B, D, or E is needed for a thermoperiodic control of flowering time in A. thaliana. 相似文献
63.
The molecular composition of square arrays 总被引:2,自引:0,他引:2
Square arrays are prominent structures in plasma membranes of brain, muscle, and kidneys with an unknown function. So far, the analysis of these arrays has been restricted to freeze fracture preparations, which have shown square arrays to contain the water channel Aquaporin-4 (AQP4). Using Blue-Native PAGE immunoblots, we provide evidence that higher-order AQP4 complexes correspond to square arrays, with the AQP4 isoform M23 playing a dominant role. Our data are consistent with the idea that square arrays consist of aggregates of AQP4 tetramers complexed with multiples of dimers. By comparison, Aquaporin-1 and Aquaporin-9 form tetramers, but not higher-order complexes. AQP4 square arrays are stable under several biochemical purification steps. Analyzing the internal composition of the higher-order complexes by 2D gels, we demonstrate that the square arrays in addition to M23 also invariably contain AQP4, M1, and a novel AQP4 isoform that we call Mz. The visualization AQP4 square arrays by a rapid, biochemical assay provides new insight in the molecular organization of square arrays and gives further proof of the heterogeneity of AQP4 square arrays in vivo. 相似文献
64.
Amphitetranychus quercivorus (Ehara and Gotoh, 1990) was first identified in Mongolian oak tree Quercus mongolica in Daegu, Korea. In comparison to A. viennensis (Zacher), A. quercivorus was much shorter in the distal, dorsally directed portion of the male aedeagus, as well as smaller and less folded in the distal anastomosed portion of the female peritreme. Comparison of the nucleotide sequences of ITS2 and COI revealed 90% and 89% similarities, respectively, between the two species. In addition, species-specific primer sets for each species were designed using unique ITS2 sequences and then used to diagnose these two species under plant quarantine. We report the Korean name of this species as “Sin-gal-na-mu-eung-ae”. 相似文献
65.
J W Sperow O A Moe J W Ridlington L G Butler 《The Journal of biological chemistry》1973,248(6):2062-2065
66.
Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation 总被引:13,自引:5,他引:8
mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge. Sequences more or less similar to mscL are found in other bacteria, but the degree of conserved function has been unclear. We subcloned and expressed these putative homologues in E . coli and examined their products under patch clamp. Here, we show that each indeed encodes a conserved mechanosensitive channel activity, consistent with the interpretation that this is an important and primary function of the protein in a wide range of bacteria. Although similar, channels of different bacteria differ in kinetics and their degree of mechanosensitivity. Comparison of the primary sequence of these proteins reveals two highly conserved regions, corresponding to domains previously shown to be important for the function of the wild-type E . coli channel, and a C-terminal region that is not conserved in all species. This structural conservation is providing insight into regions of this molecule that are vital to its role as a mechanosensitive channel and may have broader implications for the understanding of other mechanosensitive systems. 相似文献
67.
Intracellular acidosis activates c-Src 总被引:3,自引:0,他引:3
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