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161.
Wildfire is a major disturbance in Baikiaea plurijuga Harms woodland savannas. We tested the hypothesis that the timing and intensity of herbivory influence fuel loads. We used three stocking rates namely light (three cows and four goats ha?1), medium (six cows and eight goats ha?1) and heavy (eleven cows and sixteen goats ha?1) and three times of grazing namely early‐, middle‐ and late‐growing seasons. Season of grazing and stocking rate influenced herbaceous phytomass. Phytomass was generally the highest (53.5 g DM m?2) in paddocks grazed during the early growing season and the lowest (27.8 g DM m?2) in those grazed during the late growing season. Phytomass was also generally the highest (40.4 g DM m?2) in lightly stocked paddocks and the lowest (32.7 g DM m?2) in heavily stocked ones. Litter mass was the lowest (160.8 g DM m?2) in paddocks grazed during the early season whereas there were no differences in ungrazed paddocks and those grazed during either mid‐ or late growing seasons (205.4 g DM m?2). There was a negative relationship between litter mass and stocking rate. Baikiaea Benth. woodlands should be grazed during either the mid‐ or late‐growing season at stocking rates greater than 0.1 LU ha?1 to reduce grass fuel loads. 相似文献
162.
Bacterial concentration and diversity was assessed in a moderately acidic (pH 5.1) anaerobic groundwater contaminated by chlorosolvent-containing DNAPL at a Superfund site located near Baton Rouge, Louisiana. Groundwater analysis revealed a total aqueous-phase chlorosolvent concentration exceeding 1000 mg L(-1), including chloroethanes, vinyl chloride, 1,2-dichloropropane, and hexachloro-1,3-butadiene as the primary contaminants. Direct counting of stained cells revealed more than 3 x 10(7) cells mL(-1) in the groundwater, with 58% intact and potentially viable. Universal and 'Dehalococcoides'-specific 16S rRNA gene libraries were created and analyzed. Universal clones were grouped into 18 operational taxonomic units (OTUs), which were dominated by low-G+C Gram-positive bacteria (62%) and included several as yet uncultured or undescribed organisms. Several unique 16S rRNA gene sequences closely related to Dehalococcoides ethenogenes were detected. Anaerobically grown isolates (168 in total) were also sequenced. These were phylogenetically grouped into 18 OTUs, of which only three were represented in the clone library. Phylogenetic analysis of isolates and the clone sequences revealed close relationships with dechlorinators, fermenters, and hydrogen producers. Despite acidic conditions and saturation or near-saturation chlorosolvent concentrations, the data presented here demonstrate that large numbers of novel bacteria are present in groundwater within the DNAPL source zone, and the population appears to contain bacterial components necessary to carry out reductive dechlorination. 相似文献
163.
Andrew?T.?Koppisch Cindy?C.?Browder Amanda?L.?Moe Jacob?T.?Shelley Blaine?A.?Kinkel Larry?E.?Hersman Srinivas?Iyer Christy?E.?RuggieroEmail author 《Biometals》2005,18(6):577-585
The siderophores of Bacillus anthracis are critical for the pathogen’s proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2
resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin,
a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin,
a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen. 相似文献
164.
Rønning B Moe B Chastel O Broggi J Langset M Bech C 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2008,178(6):779-784
Black-legged kittiwakes (BLKIs) reduce self-maintenance cost through reductions in mass-specific basal metabolic rate (BMR), body mass and the size of visceral organs during the chick-rearing period. In the present study, we measured kidney in vitro oxygen consumption and plasma 3,3',5-triiodo-L: -thyronine (T3) levels of incubating and chick-rearing female BLKIs, to test whether the decrease in BMR is caused mainly by decreased metabolic intensity or simply by reductions in the size of organs with high metabolic intensity. Body mass and body condition were lower in chick-rearing birds compared with the incubating birds. In contrast to the previous findings, however, the kidney mass did not differ between the two breeding stages. Plasma T3 levels decreased substantially during the breeding season, indicating a reduction in BMR. Over the same period, kidney mass-specific oxygen consumption decreased (by 17.2%) from the incubating to the chick-rearing stage. Thus, the reduction in BMR found in breeding BLKIs seems partly explained by adjustments in metabolic intensity of visceral organs. Lowered metabolic intensity of visceral organs would permit increased allocation of energy to offspring at the expense of their own self-maintenance. 相似文献
165.
A large dataset from 1,077 lakes in Finland, Ireland, Sweden and the United Kingdom was collated to analyse the relationship
between nutrient status and occurrence of different taxa, as well as between total phosphorus or chlorophyll and commonly
used macroinvertebrate metrics developed for river assessment. We found that most taxa were associated with mesotrophic conditions
(sensu OECD). Species associated with oligotrophic status included Baetis rhodani, Gammarus lacustris and plecopteran larvae, a group commonly associated with low nutrient status also in rivers. Species tolerant of eutrophic
conditions were the chironomid larvae (Chironomus plumosus and Cryptochironomus defectus); and two species of tubificids (Psammoryctides barbatus and Potamothrix hammoniensis). For a number of taxa the associations of benthic invertebrates with nutrient state reported in the literature were not
supported by analysis of the REBECCA data. The analysis indicated a variable response of littoral macroinvertebrates to eutrophication
pressure when using common metrics developed for macroinvertebrates in rivers. Several metrics showed significantly different
responses in lakes with different alkalinity, justifying the use of alkalinity for typing water bodies. These significant
responses suggest that benthic invertebrates may be a useful component for classification of ecological status in lakes. The
low amount of variance explained by the regressions (<30%), however, suggests that further harmonisation of sampling methods,
as well as statistically more robust assessment tools are needed to increase the comparability of datasets and to improve
the precision in the dose–response relationships. 相似文献
166.
Andersson M Okeyo G Wilson D Keizer H Moe P Blount P Fine D Dodabalapur A Duran RS 《Biosensors & bioelectronics》2008,23(6):919-923
The mechanosensitive (MS) ion channel is gated by changes in bilayer deformation. It is functional without the presence of any other proteins and gating of the channel has been successfully achieved using conventional patch clamping techniques where a voltage has been applied together with a pressure over the membrane. Here, we have for the first time analyzed the large conducting (MscL) channel in a supported membrane using only an external electrical field. This was made possible using a newly developed technique utilizing a tethered lipid bilayer membrane (tBLM), which is part of an engineered microelectronic array chip. Single ion channel activity characteristic for MscL was obtained, albeit with lower conductivity. The ion channel was gated using solely a transmembrane potential of 300 mV. Computations demonstrate that this amount of membrane potential induces a membrane tension of 12 dyn/cm, equivalent to that calculated to gate the channel in patch clamp from pressure-induced stretching of the bilayer. These results strengthen the supposition that the MscL ion channel gates in response to stress in the lipid membrane rather than pressure across it. Furthermore, these findings illustrate the possibility of using the MscL as a release valve for engineered membrane devices; one step closer to mimicking the true function of the living cell. 相似文献
167.
Magdalena Zarowiecki Yvonne‐Marie Linton Rory J. Post Michael J. Bangs Pe Than Htun Thaung Hlaing Chang Moh Seng Visut Baimai Trung Ho Ding Tho Sochantha Catherine Walton 《Molecular ecology》2014,23(10):2573-2589
Southeast Asia harbours abundant biodiversity, hypothesized to have been generated by Pliocene and Pleistocene climatic and environmental change. Vicariance between the island of Borneo, the remaining Indonesian archipelago and mainland Southeast Asia caused by elevated sea levels during interglacial periods has been proposed to lead to diversification in the littoral zone mosquito Anopheles (Cellia) sundaicus (Rodenwaldt) sensu lato. To test this biogeographical hypothesis, we inferred the population history and assessed gene flow of A. sundaicus s.l. sampled from 18 populations across its pan‐Asian species range, using sequences from mitochondrial cytochrome c oxidase subunit 1 (CO1), the internal transcribed spacer 2 (ITS2) and the mannose phosphate isomerase (Mpi) gene. A hypothesis of ecological speciation for A. sundaicus involving divergent adaptation to brackish and freshwater larval habitats was also previously proposed, based on a deficiency of heterozygotes for Mpi allozyme alleles in sympatry. This hypothesis was not supported by Mpi sequence data, which exhibited no fixed differences between brackish and freshwater larval habitats. Mpi and CO1 supported the presence of up to eight genetically distinct population groupings. Counter to the hypothesis of three allopatric species, divergence was often no greater between Borneo, Sumatra/Java and the Southeast Asian mainland than it was between genetic groupings within these landmasses. An isolation‐with‐migration (IM) model indicates recurrent gene flow between the current major landmasses. Such gene flow would have been possible during glacial periods when the current landmasses merged, presenting opportunities for dispersal along expanding and contracting coastlines. Consequently, Pleistocene climatic variation has proved a homogenizing, rather than diversifying, force for A. sundaicus diversity. 相似文献
168.
Sergio E. Ramos Moe Bakhtiari Miguel Castañeda-Zárate Veronica Iriart Tia-Lynn Ashman 《植物分类学报:英文版》2023,61(3):550-560
Ecological interactions between plants and insects are of paramount importance for the maintenance of biodiversity and ecosystem functioning. Herbicides have long been considered a threat to plant and insect populations, but global increases in intensive agriculture and availability of herbicide-resistant crops have intensified concerns about their full impact on biodiversity. Here, we argue that exposure to sublethal herbicide doses has the potential to alter plant–insect interactions as a result of disruptions in their chemical communication. This is because herbicides interfere with biosynthetic pathways and phytohormones involved in the production of several classes of plant volatiles that mediate plant–insect chemical communication. Sublethal herbicide doses can modify the morphological and life-history plant traits and affect interactions with insects. However, the potential changes in plant volatiles and their consequences for plant–insect chemical communication have not yet received as much attention. We discuss how target-site (disruptors of primary metabolism) and non-target-site (synthetic auxins) herbicides could alter the production of plant volatiles and disrupt plant–insect chemical communication. We suggest research avenues to fill in the current gap in our knowledge that might derive recommendations and applied solutions to minimize herbicides' impacts on plant–insect interactions and biodiversity. 相似文献
169.
Foster DJ Moe OW Hsia CC 《American journal of physiology. Lung cellular and molecular physiology》2004,287(6):L1107-L1115
Circulating erythropoietin (EPO) stimulates erythrocytosis, whereas organ-specific local EPO receptor (EPOR) expression has been linked to angiogenesis, tissue growth, and development. On the basis of the observation of concurrent enhancement of lung growth and erythrocyte production during exposure to chronic hypoxia, we hypothesized that a paracrine EPO system is involved in mediating lung growth. We analyzed EPOR protein expression in normal dog lung tissue during postnatal maturation and during compensatory lung growth after right pneumonectomy (PNX). Membrane-bound EPOR was significantly more abundant in the immature lung compared with mature lung and in the remaining lung 3 wk after PNX compared with matched sham controls. COOH-terminal cytosolic EPOR peptides, which were even more abundant than membrane-bound EPOR, were also upregulated in immature lung but differentially processed after PNX. Apoptosis was enhanced during both types of lung growth in direct relationship to cellular proliferation and EPOR expression. We conclude that both developmental and compensatory lung growth involve paracrine EPO signaling with parallel upregulation but differential processing of EPOR. 相似文献
170.
The substantial increase in elephant populations across many areas in southern Africa over past decades is prompting concerns about the effects on biodiversity. We investigated the outcomes of elephant disturbance on tree-species presence, density, and richness, and on alpha and beta diversity within riparian woodland in Chobe National Park, Botswana. We enumerated all tree species occurring in 32 plots (0.06 ha) along the Chobe riverfront. Plots were stratified by soil type (nutrient-rich alluvium vs. nutrient-poor Kalahari sand covering alluvium) and elephant impact (high vs. low impact on both soil types). We tested four predictions: elephants reduce tree density, richness, and alpha diversity; beta diversity is greater in vegetation subjected to high elephant impact; elephant impact on tree-species composition is greater on nutrient-poor than on nutrient-rich soil; and the loss or decline of abundant tree species on heavily disturbed sites is offset by an increase in abundance of functionally similar species, ones that are minor on lightly disturbed sites. Elephant browsing substantially affected tree-species composition, reducing density, species richness, evenness, and alpha diversity but had no effect on beta diversity. The dominant species on relatively undisturbed areas were partly replaced by functionally similar species on heavily disturbed sites. Soil type influenced species composition on lightly disturbed sites but was less important at higher elephant densities. Our findings are important for areas with extreme dry-season densities of elephants but should not be extrapolated to infer purported effects of elephants on tree diversity at lower densities. 相似文献