全文获取类型
收费全文 | 468篇 |
免费 | 31篇 |
专业分类
499篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 9篇 |
2020年 | 4篇 |
2019年 | 10篇 |
2018年 | 15篇 |
2017年 | 8篇 |
2016年 | 21篇 |
2015年 | 18篇 |
2014年 | 25篇 |
2013年 | 40篇 |
2012年 | 23篇 |
2011年 | 20篇 |
2010年 | 14篇 |
2009年 | 19篇 |
2008年 | 30篇 |
2007年 | 21篇 |
2006年 | 16篇 |
2005年 | 23篇 |
2004年 | 17篇 |
2003年 | 16篇 |
2002年 | 16篇 |
2001年 | 12篇 |
2000年 | 11篇 |
1999年 | 9篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 9篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1991年 | 5篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 5篇 |
1977年 | 2篇 |
1974年 | 2篇 |
1972年 | 5篇 |
1970年 | 1篇 |
1964年 | 1篇 |
1962年 | 1篇 |
1952年 | 1篇 |
排序方式: 共有499条查询结果,搜索用时 10 毫秒
11.
B H Weber K Willeford J G Moe D Piszkiewicz 《Biochemical and biophysical research communications》1979,86(2):252-258
Cibacron Blue F3GA from several commercial sources is shown to be heterogeneous. This crude dye inactivates both phosphoglycerate kinase and isoleucyl-tRNA synthetase. Purification of Cibacron Blue F3GA to homogeneity results in a dramatic decrease in inactivation of these enzymes. The inactivation is shown to be due to covalent modification of phosphoglycerate kinase and probably isoleucyl-tRNA synthetase by a minor component present in crude Cibacron Blue F3GA. 相似文献
12.
Woody plant encroachment alters the structure and function of rangeland ecosystems. The objective of this study was to explore the association between woody plant encroachment and various ecosystem properties (i.e. vascular plant species diversity, richness, evenness, soil organic matter, herbaceous biomass, leaf litter and bare ground cover) in a semiarid savanna rangeland, and also to test whether the relationships were influenced by woody species composition, elevation and site. We carried out a vegetation survey in four rangeland sites in the lower Omo region of southwestern Ethiopia, and regressed each one of the ecosystem properties, separately, against woody plant density, elevation and site using multiple linear regressions. We found that vascular plant species diversity, richness and evenness increased with woody plant density, most likely due to increased spatial heterogeneity and soil microclimate improvement. Bare ground cover increased significantly, whereas herbaceous biomass and soil organic matter did not respond to woody encroachment. In a subsequent investigation, we used a redundancy analysis to assess whether ecosystem properties were influenced by the identity of encroaching woody plant species. Species diversity and richness responded positively to Lannea triphylla, whereas leaf litter responded positively to Grewia tenax and G. villosa. Our findings suggest that woody plant encroachment in a semiarid rangeland does alter ecosystem properties. However, its impact is highly variable, influenced by a set of factors including the level of encroachment and identity of encroaching woody species. 相似文献
13.
Five fungal species, Cladosporium resinae (ATCC 34066), Cladosporium sphaerospermum (ATCC 200384), Exophiala lecanii-corni (CBS 102400), Mucor rouxii (ATCC 44260), and Phanerochaete chrysosporium (ATCC 24725), were tested for their ability to degrade nine compounds commonly found in industrial off-gas emissions. Fungal cultures inoculated on ceramic support media were provided with volatile organic compounds (VOCs) via the vapor phase as their sole carbon and energy sources. Compounds tested included aromatic hydrocarbons (benzene, ethylbenzene, toluene, and styrene), ketones (methyl ethyl ketone, methyl isobutyl ketone, and methyl propyl ketone), and organic acids ( n-butyl acetate, ethyl 3-ethoxypropionate). Experiments were conducted using three pH values ranging from 3.5 to 6.5. Fungal ability to degrade each VOC was determined by observing the presence or absence of visible growth on the ceramic support medium during a 30-day test period. Results indicate that E. lecanii-corni and C. sphaerospermum can readily utilize each of the nine VOCs as a sole carbon and energy source. P. chrysosporium was able to degrade all VOCs tested except for styrene under the conditions imposed. C. resinae was able to degrade both organic acids, all of the ketones, and some of the aromatic compounds (ethylbenzene and toluene); however, it was not able to grow utilizing benzene or styrene under the conditions tested. With the VOCs tested, M. rouxiiproduced visible growth only when supplied with n-butyl acetate or ethyl 3-ethoxypropionate. Maximum growth for most fungi was observed at a pH of approximately 5.0. The experimental protocol utilized in these studies is a useful tool for assessing the ability of different fungal species to degrade gas-phase VOCs under conditions expected in a biofilter application. 相似文献
14.
在我国腹泻患儿中发现诺瓦克样病霉感染 总被引:6,自引:0,他引:6
诺瓦克病毒是引起无菌性急性胃肠炎爆发的重要病原。1990年10月至1991年1月从河南省急性腹泻门诊患儿便样中发现了诺瓦克样病毒。经电镜观察,病毒直径约为28nm,病毒壳似由有结构的亚单位组成,进一步用诺瓦克病毒特异的寡核苷酸引物做逆转录一聚合酶链式反应(RT-PCR),检定为阳性。由PCR产物测得的核苷酸序列与诺瓦克病毒原型株相应序列比较,同源性为72%。以上结果证实,在我国腹泻病人中有诺瓦克样病毒感染。这对我国病毒性胃肠炎流行的防治研究具有重要意义。 相似文献
15.
Ten‐Yang Yen Richard Wong Donald Pizzo Moe Thein Bruce A. Macher Leslie C. Timpe 《Proteomics》2020,20(15-16)
This study identifies the main changes in protein expression in human breast tumors compared to normal breast tissue. Malignant tumors (32) and normal breast tissue samples (23), from formaldehyde‐fixed, paraffin‐embedded specimens are subjected to discovery proteomics using liquid chromatography/tandem mass spectrometry, with spectral counts for quantitation. The dataset contains 1406 proteins. Differential expression is measured using a method that takes advantage of estimates of the percentage of tumor on a slide. This analysis shows that the major classes of proteins over‐expressed by tumors are RNA‐binding, heat shock and DNA repair proteins. RNA‐binding proteins, including heterogeneous nuclear ribonucleoproteins (HNRNPs), SR splice factors (SRSF) and elongation factors form the largest group. Comparison with results from another study demonstrates that the RNA‐binding proteins are associated specifically with malignant transformation, rather than with cell proliferation. HNRNP and SRSF proteins help define splice sites in normal cells. Their over‐expression may dysregulate splicing, which in turn has the potential to promote malignant transformation. 相似文献
16.
Deguchi Ryo Fujimoto Moe Sekiyama Hiroshi Sawamura Shigehito 《Sleep and biological rhythms》2021,19(3):277-283
Sleep and Biological Rhythms - Patients with chronic pain develop peripheral neuropathy and experience sleep disturbance. Yokukansan is used to treat insomnia and control neuropathic pain. We... 相似文献
17.
Assessment of human adenovirus removal by qPCR in an advanced water reclamation plant in Georgia,USA
P. Liu O. Herzegh M. Fernandez S. Hooper W. Shu J. Sobolik R. Porter N. Spivey C. Moe 《Journal of applied microbiology》2013,115(1):310-318
Aims
To assess human adenoviruses (HAdVs) removal in an advanced wastewater treatment facility and compare two parallel tertiary treatment methods for the removal of HAdVs.Methods and Results
Tangential flow ultrafiltration was used to concentrate the water samples, and HAdVs were precipitated by polyethylene glycol. HAdVs were detected only by TaqMan real‐time PCR, and HAdV genotype was determined by DNA sequence. HAdVs were detected in 100% of primary clarification influent, secondary clarification effluent and granular media (GM) filtration effluent samples but only in 31·2% of membrane filtration (MF) effluent and 41·7% of final effluent (FE) samples, respectively. The average HAdVs loads were significantly reduced along the treatments but HAdVs were still present in FE. Comparison of two parallel treatments (GM vs MF) showed that MF was technically superior to GM for the removal of HAdVs.Conclusions
These findings indicate that adenoviruses are not completely removed by treatment processes. MF is a better treatment for removal of adenoviruses than GM filtration. Because only qPCR was used, the results only indicate the removal of adenovirus DNA and not the infectivity of viruses.Significance and Impact of the Study
Presence of HAdVs in FE by qPCR suggests a potential public health risk from exposure to the treated wastewater and using the FE for recreational or water reuse purposes should be cautious. 相似文献18.
19.
A Systematic Review of Risk Factors Associated with Surgical Site Infections among Surgical Patients
Ellen Korol Karissa Johnston Nathalie Waser Frangiscos Sifakis Hasan S. Jafri Mathew Lo Moe H. Kyaw 《PloS one》2013,8(12)