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91.
92.
Atsushi Takashima Atsushi Kume Shigejiro Yoshida Takuhiko Murakami Tsuyoshi Kajisa Nobuya Mizoue 《Ecological Research》2009,24(5):1003-1011
The mountain zone of Yakushima Island is covered with a mixed conifer-broadleaved forest dominated by old-growth Cryptomeria japonica (L.f.) D. Don trees. Even though Yakushima Island has been frequently struck by typhoons with wind velocities exceeding 55 m s−1, the Cr. japonica trees in the mountain zone have survived for thousands of years without fatal damage. To evaluate the effect of storms on
tree growth, the relationships between the stem diameter at breast height (DBH) and the heights of Cr. japonica and coexistent tree species were investigated. Two models based on an expanded allometric equation and a discontinuous piecewise
allometric equation, respectively, to represent DBH–height relationships were evaluated. In all plots, the DBH–height relationship
of Cr. japonica was discontinuous between small DBH and large DBH individuals. The tops of the large DBH individuals of Cr. japonica were lost to strong winds. However in each instance, they occupied the highest position in the canopy, even if they had lost
their tops. In contrast, the DBH–height relationships of subcanopy broadleaved species were continuous in many plots and the
equilibrium heights of the dominant broadleaved species were similar and almost in the same order regardless of the canopy
heights of Cr. japonica. These results revealed a constant vertical structure in the Cr. japonica forest on Yakushima Island. Our results demonstrate a vertical niche segregation in the forest under high wind pressures
and such vertical structure enables effective use of forest space and increases the basal area density. 相似文献
93.
Eduard A. Shuralev Nikolai D. Shamaev Malik N. Mukminov Kisaburo Nagamune Yuji Taniguchi Taizo Saito Katsuya Kitoh Marina I. Arleevskaya Anastasiya Yu. Fedotova Diana R. Abdulmanova Natalya M. Aleksandrova Marina A. Efimova Aynur I. Yarullin Anna R. Valeeva Kamil S. Khaertynov Yasuhiro Takashima 《Parasitology international》2018,67(2):112-114
Toxoplasmosis, a most common zoonosis, is caused by the protozoan parasite Toxoplasma gondii. However, there is little epidemiological information on T. gondii infections in humans and livestock animals in Russia. Therefore, in this study, the seroprevalence of T. gondii in goats in Russia was investigated. A total of 216 goats from 32 farms were investigated and 95 of them were seropositive for T. gondii. The difference in seroprevalence between the examined regions was not statistically significant. We next collected serum samples from 99 cats and 181 humans in Kazan city, the state capital of the Republic of Tatarstan, Russia, and examined their T. gondii seroprevalences. Thirty-nine of the 99 cat samples and 56 of the 181 human samples showed seropositivity. Logistical regression analysis revealed that the cat breeding history of the human subjects, but not their sex or age is a significant risk factor for T. gondii seropositivity. These findings suggest that the natural environment in Russia may be widely polluted with T. gondii oocysts shed by cats, and ingestion of these oocysts provides a major route for human infection with this parasite. 相似文献
94.
A male gametocyte osmiophilic body and microgamete surface protein of the rodent malaria parasite Plasmodium yoelii (PyMiGS) plays a critical role in male osmiophilic body formation and exflagellation
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Mayumi Tachibana Tomoko Ishino Eizo Takashima Takafumi Tsuboi Motomi Torii 《Cellular microbiology》2018,20(5)
Anopheles mosquitoes transmit Plasmodium parasites of mammals, including the species that cause malaria in humans. Malaria pathology is caused by rapid multiplication of parasites in asexual intraerythrocytic cycles. Sexual stage parasites are also produced during the intraerythrocytic cycle and are ingested by the mosquito, initiating gametogenesis and subsequent sporogonic stage development. Here, we present a Plasmodium protein, termed microgamete surface protein (MiGS), which has an important role in male gametocyte osmiophilic body (MOB) formation and microgamete function. MiGS is expressed exclusively in male gametocytes and microgametes, in which MiGS localises to the MOB and microgamete surface. Targeted gene disruption of MiGS in a rodent malaria parasite Plasmodium yoelii 17XNL generated knockout parasites (ΔPyMiGS) that proliferate normally in erythrocytes and form male and female gametocytes. The number of MOB in male gametocyte cytoplasm is markedly reduced and the exflagellation of microgametes is impaired in ΔPyMiGS. In addition, anti‐PyMiGS antibody severely blocked the parasite development in the Anopheles stephensi mosquito. MiGS might thus be a potential novel transmission‐blocking vaccine target candidate. 相似文献
95.
96.
The capacitance of the membrane of phospholipid vesicles and the electrical properties of the vesicle interior have been determined. To this end the electrical properties of phospholipid vesicles have been investigated over a frequency range extending from 1 kHz to 100 MHz. The dielectric behavior is characterized by two dispersions, one placed between 1 kHz and 1 MHz and the other between 1 and 100 MHz. The relaxational behavior at low frequencies is explained by counterion movement tangential to the vesicle surface and a reasonable value for the fixed charge of the vesicles is calculated from the dispersion magnitude. The relaxation at high frequencies is of the Maxwell-Wagner type and appears caused by the phospholipid bilayer bounding the interior phase of the vesicles. It is consistent with the existence of a closed bilayer with a capacitance of about 2 μF/cm2 and an internal phase similar to the vesicle suspending medium. There is no indication of other than normally structured water inside the small vesicles. 相似文献
97.
Maeda S Sahara N Saito Y Murayama M Yoshiike Y Kim H Miyasaka T Murayama S Ikai A Takashima A 《Biochemistry》2007,46(12):3856-3861
Neurofibrillary tangles (NFTs) are pathological hallmarks of several neurodegenerative disorders, including Alzheimer's disease (AD). NFTs are composed of microtubule-binding protein tau, which assembles to form paired helical filaments (PHFs) and straight filaments. Here we show by atomic force microscopy that AD brain tissue and in vitro tau form granular and fibrillar tau aggregates. CD spectral analysis and immunostaining with conformation-dependent antibodies indicated that tau may undergo conformational changes during fibril formation. Enriched granules generated filaments, suggesting that granular tau aggregates may be an intermediate form of tau fibrils. The amount of granular tau aggregates was elevated in prefrontal cortex of Braak stage I cases compared to that of Braak stage 0 cases, suggesting that granular tau aggregation precedes PHF formation. Thus, granular tau aggregates may be a relevant marker for the early diagnosis of tauopathy. Reducing the level of these aggregates may be a promising therapy for tauopathies and for promoting healthy brain aging. 相似文献
98.
Villeneuve M Kawai M Watanabe M Aoyagi Y Hitotsuyanagi Y Takeya K Gouda H Hirono S Minnikin DE Nakahara H 《Biochimica et biophysica acta》2007,1768(7):1717-1726
Phase diagrams of Langmuir monolayers of oxygenated mycolic acids, i.e. methoxy mycolic acid (MeO-MA), ketomycolic acid (Keto-MA), and artificially obtained deoxo-mycolic acid (deoxo-MA) from Mycobacterium bovis BCG were obtained by thermodynamic analysis of the surface pressure (pi) vs. average molecular area (A) isotherms. At lower temperatures and lower surface pressures, both Keto- and MeO-MAs formed rigid condensed monolayers where each MA molecule was considered to be in a 4-chain form, in which the three carbon chain segments due to bending of the 3-hydroxy aliphatic carboxylate chain and the 2-side chain were in compact parallel arrangement. At higher temperatures and surface pressures, MeO-MA and deoxo-MA tended to take stretched-out conformations in which the 3-hydroxy aliphatic carboxylate chain was more or less in an extended form, but Keto-MA retained the original 4-chain structure. The thickness measurement of the monolayers in situ by ellipsometry at different pi values and temperatures supported the above conclusions derived from the phase diagrams. The enthalpy changes associated with the phase transitions of MeO-MA and deoxo-MA implied that the MeO-MA needed larger energy to change from a compact conformation to an extended one, possibly and partly due to the dehydration of the methoxy group from water surface involved. Molecular dynamics studies of MA models derived from Monte Carlo calculations were also performed, which confirmed the conformational behavior of MAs suggested by the thermodynamic studies on the Langmuir monolayers. 相似文献
99.
Induction of antigen-specific immunosuppression by CD95L cDNA-transfected 'killer' dendritic cells. 总被引:8,自引:0,他引:8
Dendritic cells (DCs) are special subsets of antigen-presenting cells characterized by their highly potent capacity to activate immunologically naive T cells. Here we report that DCs that are transfected with CD95 ligand (CD95L) cDNA, called 'killer' DCs, deliver death signals, instead of activation signals, to T cells after antigen-specific interaction. Injection of antigen-pulsed killer DCs into mice before sensitization induced antigen-specific immunosuppression. When administered after sensitization, killer DCs suppressed immune responses almost completely after subsequent challenge. Thus, killer DCs represent an entirely new immunomodulatory protocol, which may become directly applicable in preventing and even treating T cell-mediated inflammatory diseases. 相似文献
100.
The main original plant of the traditional Chinese drug Mianbeixian has been considered as Dioscorea septemloba Thunb. for a long time. Based on morphological, light microscopical, scanning electron microscopical, chromatographical and ultra-violet spectroscopical examinations and in comparison with the Japanese material, the Chinese one is proposed as a newspecies-Dioscorea spongiosa. 相似文献