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321.
Akihiro Suemasa Mizuki Watanabe Takaaki Kobayashi Hiroe Suzuki Hayato Fukuda Masabumi Minami Satoshi Shuto 《Bioorganic & medicinal chemistry letters》2018,28(20):3395-3399
We previously designed and synthesized a series of cyclopropane-based conformationally restricted analogues of γ-aminobutyric acid (GABA). The study demonstrated that the critical conformation of the analogues that selectively active to betaine/GABA transporter 1 (BGT1) subtype is the trans-syn-form, in which the amino and carboxyl groups are in trans-configuration and the cyclopropane ring and the carboxyl group are in syn-arrangement. In this study, we designed and synthesized cyclopropane-based GABA analogues, which were conformationally restricted in the trans-syn-form by cyclopropylic strain based on the stereochemistry of the carbon adjacent to cyclopropane. Their conformation was confirmed as the syn-form by calculations and NMR studies, and their pharmacological evaluation clarified that compounds 11a and 11d had the BGT1 selectivity, although their inhibitory effects were insufficient. 相似文献
322.
Anja Zehrmann Barbara H?rtel Franziska Glass Eszter Bayer-Császár Toshihiro Obata Etienne Meyer Axel Brennicke Mizuki Takenaka 《The Journal of biological chemistry》2015,290(10):6445-6456
RNA editing in plastids and mitochondria of flowering plants requires pentatricopeptide repeat proteins (PPR proteins) for site recognition and proteins of the multiple organellar RNA editing factor (MORF) family as cofactors. Two MORF proteins, MORF5 and MORF8, are dual-targeted to plastids and mitochondria; two are targeted to plastids, and five are targeted to mitochondria. Pulldown assays from Arabidopsis thaliana tissue culture extracts with the mitochondrial MORF1 and the plastid MORF2 proteins, respectively, both identify the dual-targeted MORF8 protein, showing that these complexes can assemble in the organelles. We have now determined the scope of potential interactions between the various MORF proteins by yeast two-hybrid, in vitro pulldown, and bimolecular fluorescence complementation assays. The resulting MORF-MORF interactome identifies specific heteromeric MORF protein interactions in plastids and in mitochondria. Heteromers are observed for MORF protein combinations affecting a common site, suggesting their functional relevance. Most MORF proteins also undergo homomeric interactions. Submolecular analysis of the MORF1 protein reveals that the MORF-MORF protein connections require the C-terminal region of the central conserved MORF box. This domain has no similarity to known protein modules and may form a novel surface for protein-protein interactions. 相似文献
323.
Mari Morimoto Mizuki Mitsukawa Chisato Fujiwara Yukio Kawamura 《Bioscience, biotechnology, and biochemistry》2019,83(3):498-501
Inhibition of mRNA processing, including splicing in the nucleus, is a potential anti-cancer candidate. To obtain mRNA processing inhibitors, we have screened for active constituents from spices. Ginger, clove, and cinnamon showed an inhibitory effect on mRNA processing in the nucleus. Two components in ginger, 6-gingerol and 6-shogaol, exhibited the inhibition of mRNA processing. 相似文献
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Ichthyological Research - Dampierosa daruma Whitley 1932 and Erosa erosa (Cuvier in Cuvier and Valenciennes 1829) (Synanceiidae) were redescribed on the basis of both primary type and non-type... 相似文献
326.
Matsunuma Mizuki Uesaka Kenta Yamakawa Takeshi Endo Hiromitsu 《Ichthyological Research》2022,69(3):299-351
Ichthyological Research - A taxonomic review of Plectrogenium (Teleostei: Plectrogeniidae) disclosed 10 valid species, eight being new (most previously identified as P. nanum Gilbert 1905): P.... 相似文献
327.
Ichthyological Research - A taxonomic revision of the genus Parapterois (Scorpaenidae: Pteroinae) resulted in recognition of three valid species, Pa. heterura (Bleeker 1856) (type locality:... 相似文献
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We propose a method of activating an enzyme utilizing heat generation from ferromagnetic particles under an ac magnetic field. We immobilize α-amylase on the surface of ferromagnetic particles and analyze its activity. We find that when α-amylase/ferromagnetic particle hybrids, that is, ferromagnetic particles, on which α-amylase molecules are immobilized, are subjected to an ac magnetic field, the particles generate heat and as a result, α-amylase on the particles is heated up and activated. We next prepare a solution, in which α-amylase/ferromagnetic particle hybrids and free, nonimmobilized chitinase are dispersed, and analyze their activities. We find that when the solution is subjected to an ac magnetic field, the activity of α-amylase immobilized on the particles increases, whereas that of free chitinase hardly changes; in other words, only α-amylase immobilized on the particles is selectively activated due to heat generation from the particles. 相似文献
330.