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761.
Electron transport in spinach chloroplasts treated with cholateor Tris in the presence and absence of 20% glycerol was measured.Glycerol suppressed the inhibitory action of cholate and Trison the donor side of photosystem II and also restored the Hillactivity previously lowered by cholate. This restoration requiredthe cholate-extract from the chloroplasts. (Received November 17, 1982; Accepted July 25, 1983) 相似文献
762.
Using adhesive drapes and a disposable suction drain, a new method for the dressing of free skin grafts has been devised. The graft is compressed by pressure that is equivalent to the negative pressure of the suction drain. This method can apply uniform and constant pressure on the graft. Moreover, the graft can be observed through a transparent drape so that the existence of hematomas can be detected easily. 相似文献
763.
Summary We isolated a series of Tn5-insertional mutants from the mini-F plasmid, which has a deletion in the origin II region and replicates exclusively from origin I, and found that the mutants that had Tn5 in either the F4 or the F5 gene were defective in their replication. It is concluded that, in addition to the F3 gene on which we have reported previously, both the F4 and the F5 genes are essential for the replication from origin I. 相似文献
764.
765.
Hideyuki Matsuura Yosuke Yamamoto Misa Muraoka Kenji Akaishi Yasuhisa Hori Kanoko Uemura Naoki Tsuji Kazuo Harada Kazumasa Hirata Takeshi Bamba Hitoshi Miyasaka Kouichi Kuroda Mitsuyoshi Ueda 《Biotechnology progress》2013,29(5):1197-1202
The development of simple, portable, inexpensive, and rapid analytical methods for detecting and monitoring toxic heavy metals are important for the safety and security of humans and their environment. Herein, we describe the application of phytochelatin (PC) synthase, which plays a critical role in heavy metal responses in higher plants and green algae, in a novel fluorescent sensing platform for cadmium (Cd). We first created surface‐engineered yeast cells on which the PC synthase from Arabidopsis (AtPCS1) was displayed with retention of enzymatic activity. The general concept for the sensor is based on the Cd level‐dependent synthesis of PC2 from glutathiones by AtPCS1‐displaying yeast cells, followed by simple discriminative detection of PC2 via sensing of excimer fluorescence of thiol‐labeling pyrene probes. The intensity of excimer fluorescence increased in the presence of Cd up to 1.0 μM in an approximately dose‐dependent manner. This novel biosensor achieved a detection limit of as low as 0.2 μM (22.5 μg/L) for Cd. Although its use may be limited by the fact that Cu and Pb can induce cross‐reaction, the proposed simple biosensor holds promise as a method useful for cost‐effective screening of Cd contamination in environmental and food samples. The AtPCS1‐displaying yeast cells also might be attractive tools for dissection of the catalytic mechanisms of PCS. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1197–1202, 2013 相似文献
766.
T Inoue K Kikuchi K Hirose M Iino T Nagano 《Bioorganic & medicinal chemistry letters》1999,9(12):1697-1702
IP3 analogs were synthesized by the modification of phosphate at the 1-position, and their affinity for the IP3 receptor was analyzed by means of surface plasmon resonance measurements. Our results suggest that a hydrophobic and charged moiety linked to this position enhances the affinity for the IP3 receptor. 相似文献