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991.
Isolated and cultured neonatal cardiac myocytes contract spontaneously and cyclically. The contraction rhythms of two isolated cardiac myocytes, each of which beats at different frequencies at first, become synchronized after the establishment of mutual contacts, suggesting that mutual entrainment occurs due to electrical and/or mechanical interactions between two myocytes. The intracellular concentration of free Ca(2+) also changes rhythmically in association with the rhythmic contraction of myocytes (Ca(2+) oscillation), and such a Ca(2+) oscillation was also synchronized among cultured cardiac myocytes. In this study, we investigated whether intercellular communication other than via gap junctions was involved in the intercellular synchronization of intracellular Ca(2+) oscillation in spontaneously beating cultured cardiac myocytes. Treatment with either blockers of gap junction channels or an un-coupler of E-C coupling did not affect the intercellular synchronization of Ca(2+) oscillation. In contrast, treatment with a blocker of P2 purinoceptors resulted in the asynchronization of Ca(2+) oscillatory rhythms among cardiac myocytes. The present study suggested that the extracellular ATP-purinoceptor system was responsible for the intercellular synchronization of Ca(2+) oscillation among cardiac myocytes. 相似文献
992.
Pack SP Kamisetty NK Nonogawa M Devarayapalli KC Ohtani K Yamada K Yoshida Y Kodaki T Makino K 《Nucleic acids research》2007,35(17):e110
Oxanine having an O-acylisourea structure was explored to see if its reactivity with amino group is useful in DNA microarray fabrication. By the chemical synthesis, a nucleotide unit of oxanine (Oxa-N) was incorporated into the 5′-end of probe DNA with or without the -(CH2)n- spacers (n = 3 and 12) and found to immobilize the probe DNA covalently onto the NH2-functionalized glass slide by one-pot reaction, producing the high efficiency of the target hybridization. The methylene spacer, particularly the longer one, generated higher efficiency of the target recognition although there was little effect on the amount of the immobilized DNA oligomers. The post-spotting treatment was also carried out under the mild conditions (at 25 or 42°C) and the efficiencies of the immobilization and the target recognition were evaluated similarly, and analogous trends were obtained. It has also been determined under the mild conditions that the humidity and time of the post-spotting treatment, pH of the spotting solution and the synergistic effects with UV-irradiation largely contribute to the desired immobilization and resulting target recognition. Immobilization of DNA oligomer by use of Oxa-N on the NH2-functionalized surface without any activation step would be employed as one of the advanced methods for generating DNA-conjugated solid surface. 相似文献
993.
994.
Androgen Receptor Silences Thioredoxin‐interacting Protein and Competitively Inhibits Glucocorticoid Receptor‐Mediated Apoptosis in Pancreatic β‐Cells
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995.
Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signaling in Brassica rapa 总被引:2,自引:0,他引:2
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Kakita M Murase K Iwano M Matsumoto T Watanabe M Shiba H Isogai A Takayama S 《The Plant cell》2007,19(12):3961-3973
996.
997.
Ohashi M Horie K Hoshikawa Y Nagata K Osaki M Ito H Sairenji T 《Microbes and infection / Institut Pasteur》2007,9(2):150-159
As a new model to elucidate molecular mechanisms in Epstein-Barr virus (EBV) activation, we tested the tetracycline-inducible (Tet-On)/BZLF1-oriP plasmid system in Raji cells. Cells transfected with this Tet-On plasmid did not activate EBV by doxycycline and surprisingly EBV latency was disrupted with large amounts of BMRF1 protein (EA-D) being accumulated in the cells. Brilliant EA-D fluorescence was markedly condensed in small sized cells, intra-cellular vesicles, and extra-cellular particles. Scanning electron microscopy demonstrated the extra-cellular particles to be covered with a membrane. EA-D molecules of 58, 50, 48, and 44kDa were expressed in the cells. The high (58 and 50kDa) and low (48 and 44kDa) EA-D molecules appeared in the early and late stages, respectively. Low EA-D molecules were detected mostly in EA-D positive cells separated into the heaviest density layer of a discontinuous Percoll gradient. Such molecules could be created from high EA-D molecules by protein phosphatase treatment. The EA-D molecules that appeared similar were detected in EBV-activated P3HR-1 and Akata cells. Several hypotheses concerning the accumulation of EA-D molecules of various polymorphic forms and their phosphorylation/dephosphorylation in this model system are presented, with possible biological and clinical relevance. 相似文献
998.
Masuzawa Toshiyuki Suzuki Teruyuki Seki Kayoko Kosugi Tsuyoshi Hibi Yuriko Yamamoto Mineko Takada Jitsuya Matsushita Rokuji Yanada Mitsuru 《Biological trace element research》1999,(1):331-342
Phytoplankton samples were collected during spring bloom of diatoms from three coastal areas of Japan using a NORPAC P-25
net (25-Μm opening) with a NGG52 prenet (335-Μm opening), and 25 major and trace elements have been analyzed by INAA. Concentration
ranges of analyzed phytoplankton samples are much wider than the concentration ranges compiled by Bowen (1979) except for
As, and data of marine phytoplankton samples for Br, Sb, Hf, Sc, La, Ce, Sm, and Eu were not included in the compilation.
The 25 analyzed elements have been categorized into three groups: elements showing positive correlation with Br, positive
correlation with Al, and no positive correlation with Br or Al. The marine phytoplankton samples have been plotted on a Masuzawa-Koyama-Terazaki
(MKT) plot and it proved that the MKT plot is applicable to marine phytoplankton samples. 相似文献
999.
Komori Sadayoshi Li Binghong Matsumura Kuniyoshi Takusagawa Masahiro Sano Soichi Kohno Isao Osada Mitsuru Sawanobori Takao Ishihara Tsukasa Umetani Ken Ijiri Hiroshi Tamura Kohji 《Molecular and cellular biochemistry》1999,199(1-2):201-208
The antiarrhythmic effect of magnesium sulfate (Mg) as well as the hemodynamics were studied using the coronary ligation and reperfusion models in rats.In the study on coronary ligation arrhythmia, i.v. administration of Mg (0.6, 2, 6, 20 and 60 \sgmaelig;mol) was conducted at 5 min after coronary ligation. Mg had an action to decrease the total number of premature ventricular contraction (PVC), the duration of ventricular tachycardia (VT), the frequency of VT and ventricular fibrillation (Vf) and the mortality ratio for 30 min after coronary ligation. In the 6-60 \sgmaelig;mol groups, significant antiarrhythmic action (p < 0.01 vs. control) was attained.In the study on reperfusion arrhythmia, i.v. administration of Mg (20, 60 and 200 \sgmaelig;mol) was conducted at 4 min after coronary ligation, and at 1 min after ligation, the coronary artery was reperfused. Mg had an action to decrease the frequency of Vf, the mortality ratio and the duration of VT and Vf and to extend the interval between the initiation of reperfusion and the occurrence of VT and Vf for 10 min after reperfusion. In the 200 \sgmaelig;mol group, significant antiarrhythmic action (p < 0.05 vs. control) was attained. Administration of Mg decreased the heart rate and blood pressure.We concluded that Mg can control myocardial ischemia-induced and reperfusion-induced arrhythmia and that sudden cardiac death which occurs as a result of arrhythmia can be prevented. 相似文献
1000.
Kohji Nakazawa Hiroshi Mizumoto Mitsuru Kaneko Hiroyuki Ijima Tomonobu Gion Mitsuo Shimada Ken Shirabe Kenji Takenaka Keizo Sugimachi Kazumori Funatsu 《Cytotechnology》1999,31(1-2):61-68
Porcine hepatocytes are used in the hybrid artificial liver support system that we are developing because of their high level
of liver functions in vitro and because human hepatocytes can not be used in Japan for ethical reasons. Spherical multicellular
aggregates or spheroids have been found to be effective in vitro for long-term maintenance of liver functions. Therefore,
we formed spherical multicellular aggregates (spheroids) of primary porcine hepatocytes using a polyurethane foam (PUF) as
a culture substratum and analyzed their drug metabolic functions in vitro. Primary porcine hepatocytes inoculated into the
pores of a flat PUF plate (25 × 25 × 1 mm), spontaneously formed spheroids within the range of 100 to 150 μm in diameter 24
to 36 h after inoculation. The formed spheroids were attached to the bottom surface of the PUF pores, and their morphology
and viability were maintained for more than 12 days. The P-450 activity in the spheroids of porcine hepatocytes was demonstrated
by detecting production of monoethylglycinexylidide from lidocaine. In addition, the conjugation enzyme activity was demonstrated
by detecting glucuronidation and sulfation of acetaminophen. These activities were maintained for 12 days at a level twice
as high as in the monolayer culture. This result shows that the porcine hepatocyte spheroids formed by using PUF can maintain
the drug metabolic functions important in a hybrid artificial liver device. Consequently, culturing porcine hepatocyte spheroids
using PUF seems to be promising for development of a hybrid artificial liver.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献