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排序方式: 共有247条查询结果,搜索用时 15 毫秒
241.
N Hirayama M Senda H Yamamoto Y Yoshikawa K Yamanouchi 《Microbiology and immunology》1985,29(1):47-54
Ten species of virus-specific RNA were detected in Vero cells infected with the FXNO strain of canine distemper virus (CDV). The largest RNA was the genome-sized RNA and the nine smaller species were polyadenylated RNAs. Similar results were obtained for nine other strains of CDV. The molecular weights of these ten RNAs were determined to be 4.61 X 10(6), 2.46 X 10(6), 1.52 X 10(6), 1.32 X 10(6), 1.19 X 10(6), 1.07 X 10(6), 0.77 X 10(6), 0.65 X 10(6), 0.58 X 10(6), and 0.48 X 10(6). By in vitro translation of the polyadenylated RNAs in a rabbit reticulocyte lysate system, three different proteins which probably correspond to H, NP, and M were synthesized from the fraction containing RNAs 7, 8, 9, and 10. 相似文献
242.
T Sakaki M Inoue S Senda K Tomita 《Biochemical and biophysical research communications》1978,83(1):21-26
The crystal and molecular structure of the title complex has been determined by X-ray diffraction methods. The crystals contain one water molecule per asymmetric unit, which plays a important role in the molecular packing by forming hydrogen bonds with two carboxyl oxygen atoms of indole-3-acetic acid and a carbamoyl nitrogen atom of the 1-methyl-3-carbamoylpyridinium cation. Prominent stacking between the indole ring and the pyridinium ring, caused by the πD?πA interaction, is observed. This overlap with substantial result may provide a model for the stacking interaction between NAD+ and the tryptophanyl residues in the proteins. 相似文献
243.
M. Senda Y. Onodera T. Kinoshita T. Mikami 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,90(7-8):914-919
Restriction fragment length polymorphisms (RFLPs) for three mitochondrial genes, coxI, coxII and atpA, were used to determine mitochondrial (mt) DNA diversity in 21 accessions of the genus Beta representing wild and cultivated species. On the basis of distribution of the RFLP patterns these Beta genotypes were assigned into six distinct chondriome groups. A high degree of heterogeneity was found to exist between the mitochondrial genomes of the sugarbeet cultivar and the wild species of Procumbentes section. The polymorphic fragments from wild Beta species were cloned and subjected to fine mapping. We found that most of the RFLPs are due to sequence rearrangements rather than point mutations. Our data also suggest that the close linkage between coxII and coxI is taxonomically localized to an evolutionary lineage that led to Vulgares and Corollinae species but not to Procumbentes species. This linkage is most likely to have arisen via the mutation(s) that inserted the DNA segment containing coxI downstream of coxII in the common ancestor of Vulgares and Corollinae species. The results are discussed with regard to the taxonomic and phylogenetic relationships of the Beta species. 相似文献
244.
Mahito Nakanishi Hiroyuki Mizuguchi Ken-ichi Ashihara Takao Senda Akiko Eguchi Akiko Watabe 《Molecular membrane biology》2013,30(1):123-127
Fusogenic liposome (FL) is a delivery system that can transfer encapsulated materials into living cells directly through membrane fusion. FL is a promising approach for gene therapy because it can deliver various genetic materials much more efficiently than other non-viral vectors without damaging the cell. FL-mediated gene transfer consists of two independent membrane fusion phenomena; generation of a FL by fusing a Sendai virus (SV) particle with a simple liposome encapsulating DNA, and successive fusion of the FL with cell membrane. The former requires viral F protein but no other special molecule on the liposomal membrane, whereas the latter may require the receptor (sialic acid) and unidentified assistant molecule(s) on the cell membrane. Further analysis suggests that these assistant molecule(s), not the receptor, may control the fusion and govern the cell specificity of FL-mediated delivery. This review has described a detailed analysis of these fusion phenomena and discussed possible applications of FL-mediated gene delivery to human gene therapy. 相似文献
245.
246.
Sencia Mitsugi; Takeda Junko; Abe Shunnosuke; Nakamura Takeshi 《Plant & cell physiology》1979,20(7):1441-1443
When an electric impulse of a few milliseconds was applied topoint-adherence protoplasts isolated from cultured cells ofRauwolfia serpentina through glass capillary microelectrodes,fusion of the protoplasts was immediately induced. This phenomenonseems to be related to transient changes in the membrane state,such as membrane excitation, induced by electrical stimulation. (Received February 22, 1979; ) 相似文献
247.
T Senda A Matsumine H Yanai T Akiyama 《The journal of histochemistry and cytochemistry》1999,47(9):1149-1158
Localization of the MCC (mutated in colorectal cancer) gene product, a cell cycle-regulating protein mutated in several colorectal tumors, in various mouse tissues was examined by immunohistochemistry and immunoelectron microscopy. MCC was localized on microvilli and in the apical cytoplasm in renal proximal tubule epithelial cells and pancreatic acinar cells. In hepatocytes, MCC was exclusively detected on microvilli. MCC was highly expressed in the cerebral cortex and the molecular layer of the cerebellar cortex and was partially associated with membrane organelles in neuronal elements. Adrenal chromaffin cells showed little expression of MCC. MCC was localized to the cell margins of ependymal cells, thyroid follicular cells, and anterior pituitary cells. In parotid acinar cells, only the apical surface was immunopositive. MCC was not expressed in skeletal and cardiac muscle. MCC was present at lateral cell borders in the duodenum and colon epithelium. In addition, the apical cytoplasm of colon epithelial cells exhibited intense immunoreactivity. The amount of MCC increased during differentiation of NGF-treated PC12 cells. In conclusion, MCC was expressed in differentiated cells and was associated with the plasma membrane and membrane organelles. In addition to the negative regulation of the cell cycle, MCC may be involved in cell differentiation. 相似文献