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排序方式: 共有407条查询结果,搜索用时 15 毫秒
91.
Kawanabe N Murata S Fukushima H Ishihara Y Yanagita T Yanagita E Ono M Kurosaka H Kamioka H Itoh T Kuboki T Yamashiro T 《Experimental cell research》2012,318(5):453-463
Embryonic stem cell-associated antigens are expressed in a variety of adult stem cells as well as embryonic stem cells. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 can be used to isolate dental pulp (DP) stem cells. DP cells showed plastic adherence, specific surface antigen expression, and multipotent differentiation potential, similar to mesenchymal stem cells (MSC). SSEA-4+ cells were found in cultured DP cells in vitro as well as in DP tissue in vivo. Flow cytometric analysis demonstrated that 45.5% of the DP cells were SSEA-4+. When the DP cells were cultured in the presence of all-trans-retinoic acid, marked downregulation of SSEA-3 and SSEA-4 and the upregulation of SSEA-1 were observed. SSEA-4+ DP cells showed a greater telomere length and a higher growth rate compared to ungated and SSEA-4- cells. A clonal assay demonstrated that 65.5% of the SSEA-4+ DP cells had osteogenic potential, and the SSEA-4+ clonal DP cells showed multilineage differentiation potential toward osteoblasts, chondrocytes, and neurons in vitro. In addition, the SSEA-4+ DP cells had the capacity to form ectopic bone in vivo. Thus, our results suggest that SSEA-4 is a specific cell surface antigen that can be used to identify DP stem cells. 相似文献
92.
Nitta A Iura Y Tomioka H Sato I Morihira K Kubota H Morokata T Takeuchi M Ohta M Tsukamoto S Imaoka T Takahashi T 《Bioorganic & medicinal chemistry letters》2012,22(15):4951-4954
The synthesis and structure-activity relationships of ureas as CCR3 antagonists are described. Optimization starting with lead compound 2 (IC(50)=190 nM) derived from initial screening hit compound 1 (IC(50)=600 nM) led to the identification of (S)-N-((1R,3S,5S)-8-((6-fluoronaphthalen-2-yl)methyl)-8-azabicyclo[3.2.1]octan-3-yl)-N-(2-nitrophenyl)pyrrolidine-1,2-dicarboxamide 27 (IC(50)=4.9 nM) as a potent CCR3 antagonist. 相似文献
93.
Negoro K Yonetoku Y Maruyama T Yoshida S Takeuchi M Ohta M 《Bioorganic & medicinal chemistry》2012,20(7):2369-2375
Through preparation and examination of a series of novel 4-amino-2-phenylpyrimidine derivatives as agonists for GPR119, we identified 2-(4-bromophenyl)-6-methyl-N-[2-(1-oxidopyridin-3-yl)ethyl]pyrimidin-4-amine (9t). Compound 9t improved glucose tolerance in mice following oral administration and showed good pharmacokinetic profiles in rats. 相似文献
94.
A novel HSF1-mediated death pathway that is suppressed by heat shock proteins 总被引:6,自引:0,他引:6
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Hayashida N Inouye S Fujimoto M Tanaka Y Izu H Takaki E Ichikawa H Rho J Nakai A 《The EMBO journal》2006,25(20):4773-4783
95.
Tomotake H Okuyama R Katagiri M Fuzita M Yamato M Ota F 《Bioscience, biotechnology, and biochemistry》2006,70(11):2771-2774
The fatty acid composition, the lipid digestibility and protein profile of Japanese-Saanen goat's milk were characterized. Caprine milk contained substantial quantities of C(4:0) to C(10:0) fatty acids as compared with Holstein cow's milk. The lipids of the former showed significantly higher digestibility in vitro by porcine lipase than those of the latter (P<0.05). As determined by SDS-PAGE, the respective contents of alpha(s1)-casein, one of the major allergens, were 3.9% and 33.7% in caprine and bovine milk. 相似文献
96.
Tatsuya Maruyama Kenichi Onda Masahiko Hayakawa Norio Seki Takumi Takahashi Hiroyuki Moritomo Takayuki Suzuki Tetsuo Matsui Toshiyuki Takasu Itsuro Nagase Mitsuaki Ohta 《Bioorganic & medicinal chemistry》2009,17(9):3283-3294
In the search for potent and selective human β3-adrenergic receptor (AR) agonists as potential drugs for the treatment of obesity and noninsulin-dependent (type II) diabetes, a novel series of phenoxypropanolamine derivatives containing acetanilides were prepared and their biological activities were evaluated at the human β3-, β2-, and β1-ARs. Several of the analogues (21a, 21b, and 27a) exhibited potent agonistic activity at the β3-AR. Among the compounds described herein, the N-methyl-1-benzylimidazol-2-ylacetanilide derivative (21b) was found to be the most potent and selective β3-AR agonist, with an EC50 value of 0.28 μM and no agonistic activity for either the β1- or β2-AR. In addition, 21b showed significant hypoglycemic activity in a rodent diabetic model. 相似文献
97.
98.
99.
Mitsuaki Nishioka Hiroaki Takeuchi Sergio A. Con Yoshio Uehara Isao Nishimori Toshika Okumiya Yoshitaka Kumon Tetsuro Sugiura 《Microbiology and immunology》2010,54(8):442-451
To elucidate a potential role for H. pylori BabA and SabA adhesins in the pathogenesis of gastric mucosal lesions, the MBS of BabA and SabA was examined using an in‐house ABA‐ELISA. Ninety isolates from Japanese patients with gastric cancer (n= 43) and non‐cancerous (n= 47) lesions were subjected to an ABA‐ELISA which had been developed in‐house, and sequential analysis of the babA2 middle region. The BabA‐MBS was significantly higher in the cancer than the non‐cancer group (P= 0.019), but there was no significant difference for SabA‐MBS. A weak correlation between BabA‐MBS and SabA‐MBS (r= 0.418) was observed, the positive correlation being higher in the cancer than the non‐cancer group (r= 0.598 and 0.288, respectively). The isolates were classified into two groups: a BabA‐high‐binding and a BabA‐low‐binding group (in comparison to the average for BabA‐MBS). The average SabA‐MBS in the BabA‐high‐binding group was significantly higher than in the BabA‐low‐binding group (P < 0.0001). Analysis of babA2 middle region diversity (AD1–5) revealed that AD2‐type was predominant in isolates irrespective of BabA‐MBS. H. pylori BabA‐MBS might have an effect on SabA‐MBS and relate to the severity of gastric disorders, including gastric cancer. Evaluation of MBS of the combined two adhesins would be helpful for predicting damage in the H. pylori infected stomach. 相似文献
100.
Katsuki K Fujimoto M Zhang XY Izu H Takaki E Tanizawa Y Inouye S Nakai A 《FEBS letters》2004,571(1-3):187-191