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A key virulence strategy of bacterial pathogens is the delivery of multiple pathogen effector proteins into host cells during infection. The Hrp outer protein Q (HopQ1) effector from Pseudomonas syringae pv tomato (Pto) strain DC3000 is conserved across multiple bacterial plant pathogens. Here, we investigated the virulence function and host targets of HopQ1 in tomato (Solanum lycopersicum). Transgenic tomato lines expressing dexamethasone-inducible HopQ1 exhibited enhanced disease susceptibility to virulent Pto DC3000, the Pto ΔhrcC mutant, and decreased expression of a pathogen-associated molecular pattern-triggered marker gene after bacterial inoculation. HopQ1-interacting proteins were coimmunoprecipitated and identified by mass spectrometry. HopQ1 can associate with multiple tomato 14-3-3 proteins, including TFT1 and TFT5. HopQ1 is phosphorylated in tomato, and four phosphorylated peptides were identified by mass spectrometry. HopQ1 possesses a conserved mode I 14-3-3 binding motif whose serine-51 residue is phosphorylated in tomato and regulates its association with TFT1 and TFT5. Confocal microscopy and fractionation reveal that HopQ1 exhibits nucleocytoplasmic localization, while HopQ1 dephosphorylation mimics exhibit more pronounced nuclear localization. HopQ1 delivered from Pto DC3000 was found to promote bacterial virulence in the tomato genotype Rio Grande 76R. However, the HopQ1(S51A) mutant delivered from Pto DC3000 was unable to promote pathogen virulence. Taken together, our data demonstrate that HopQ1 enhances bacterial virulence and associates with tomato 14-3-3 proteins in a phosphorylation-dependent manner that influences HopQ1’s subcellular localization and virulence-promoting activities in planta.The ability to detect and mount a defense response against pathogenic microbes is vital for plant survival. Plants rely on both passive and active defenses to ward off microbial pathogens. Physical barriers, such as the cell wall and cuticle, as well as chemical barriers provide a first line of defense against microbial colonization. Unlike animals, plants do not possess a circulating immune system and rely on innate immunity for active defenses against microbial pathogens (Spoel and Dong, 2012). Plants use surface-localized receptors to recognize conserved pathogen-associated molecular patterns (PAMPs), such as bacterial flagellin, resulting in pattern-triggered immunity (PTI; Zipfel et al., 2006). Plants also use primarily intracellular nucleotide-binding domain, Leu-rich repeat containing (NLR) immune receptors to recognize pathogen effectors delivered into host cells during infection (Spoel and Dong, 2012). NLR activation results in effector-triggered immunity (ETI). A signature of ETI is the hypersensitive response (HR), a form of programmed cell death occurring at the site of infection.In order to cause disease and suppress host defense responses, gram-negative bacterial pathogens deliver effector proteins into host cells via the type III secretion system (TTSS). Plant pathogenic bacteria deliver a large number (20–40) of effectors into host cells during infection (Cui et al., 2009). Collectively, effectors are required for bacterial virulence (Lindgren et al., 1986). However, knockouts affecting individual effectors frequently have phenotypes that are subtle, likely due to functional redundancy (Cunnac et al., 2011). Alternatively, individual effectors may play an important role in bacterial survival under conditions that are not typically analyzed in the laboratory or act cooperatively with one another. Progress in understanding individual effectors’ contributions to virulence has been made by generating transgenic plants that express effectors. Multiple effectors have been shown to suppress plant innate immunity and promote bacterial growth when either transiently or stably expressed in plants (Jamir et al., 2004; Guo et al., 2009). Effector expression can also result in avirulent phenotypes when a plant NLR receptor recognizes a cognate effector and mounts an HR. Such an HR phenotype can be used to dissect important effector domains required for plant recognition and enzymatic activity.Elucidating effector targets and enzymatic activity is necessary in order to understand how they act to subvert plant immune responses and can provide elegant insight into biological processes. Significant progress has been made in elucidating the enzymatic activity of a subset of effectors. Some of the most well-characterized effectors come from Pseudomonas syringae pv tomato (Pto), the causal agent of bacterial speck on tomato (Solanum lycopersicum) and Arabidopsis (Arabidopsis thaliana). Multiple effectors can suppress immune responses by directly targeting PAMP receptors (AvrPto and AvrPtoB) or by interfering with downstream signaling processes (AvrB, AvrPphB, and HopAI1; Cui et al., 2009, 2010). The HopU1 effector interferes with RNA metabolism (Fu et al., 2007), and the HopI1 effector targets heat-shock proteins in the plant chloroplast (Jelenska et al., 2010).14-3-3s are conserved eukaryotic proteins that bind a diverse set of phosphorylated client proteins, typically at one of three distinct 14-3-3 binding motifs (Bridges and Moorhead, 2005). There are common recognition motifs for 14-3-3 proteins that contain phosphorylated Ser or Thr residues, but binding to nonphosphorylated ligands and to proteins lacking consensus motifs has been reported (Henriksson et al., 2002; Smith et al., 2011). The 14-3-3 mode I consensus motif is RXXpS/pTX and that of mode II is RXXXpS/pTXP, where X can be any amino acid and p indicates the site of phosphorylation (Smith et al., 2011). 14-3-3 proteins can also bind to the extreme C termini of proteins at the RXXpS/pTX-COOH mode III consensus motif (Smith et al., 2011). Interaction with 14-3-3s can regulate protein activity by influencing client subcellular localization, structure, and protein-protein interactions (Bridges and Moorhead, 2005). Recently, the Xanthomonas campestris XopN effector was shown to target tomato 14-3-3 isoforms, which facilitates its interaction with the tomato atypical receptor kinase1 and suppresses PTI (Kim et al., 2009; Taylor et al., 2012). Other 14-3-3s have also been shown to play a role during plant defense responses. The tomato TFT7 14-3-3 interacts with multiple mitogen-activated protein kinases to positively regulate HR induced by ETI (Oh and Martin, 2011). The Arabidopsis 14-3-3 isoform λ interacts with the RPW8.2 powdery mildew receptor and is required for complete RPW8.2-mediated resistance (Yang et al., 2009).In this study, we investigated the function of the Pto HopQ1 (for Hrp outer protein Q [also known as HopQ1-1]) effector in tomato. HopQ1 is an active effector that is transcribed and translocated via the TTSS (Schechter et al., 2004). HopQ1 induces cell death when expressed in Nicotiana benthamiana and therefore contributes to differences in host range in P. syringae pathovars on Nicotiana spp. (Wei et al., 2007; Ferrante et al., 2009). HopQ1 was also reported to slightly enhance disease symptoms (approximately 0.2 log) and bacterial virulence on bean (Phaseolus vulgaris) when expressed from P. syringae pv tabaci (Ferrante et al., 2009). Here, we generated transgenic tomato plants expressing HopQ1 that exhibited enhanced susceptibility to virulent Pto as well as the Pto
ΔhrcC mutant. HopQ1-interacting proteins were identified from tomato using coimmunoprecipitations coupled with mass spectrometry. Multiple 14-3-3 proteins were identified. HopQ1 possesses a 14-3-3 binding motif whose Ser residue is phosphorylated in planta and affects its association with the tomato 14-3-3s TFT1 and TFT5. Mutation of HopQ1’s 14-3-3 binding motif affected its ability to promote bacterial virulence. Taken together, these results indicate that phosphorylation and subsequent interaction with tomato 14-3-3 proteins affect HopQ1’s virulence-promoting activities and subcellular localization. 相似文献
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