全文获取类型
收费全文 | 142篇 |
免费 | 15篇 |
出版年
2022年 | 1篇 |
2021年 | 5篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 10篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 14篇 |
2012年 | 10篇 |
2011年 | 7篇 |
2010年 | 10篇 |
2009年 | 7篇 |
2008年 | 13篇 |
2007年 | 8篇 |
2006年 | 4篇 |
2005年 | 11篇 |
2004年 | 10篇 |
2003年 | 1篇 |
2002年 | 6篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1995年 | 3篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1976年 | 1篇 |
排序方式: 共有157条查询结果,搜索用时 31 毫秒
131.
Katarzyna?B.?Miska April?M.?Wright Rachel?Lundgren Robert?Sasaki–McClees Amy?Osterman James?M.?Gale Robert?D.?MillerEmail author 《Mammalian genome》2004,15(10):851-864
A 37-kb cosmid containing two complete major histocompatibility complex (MHC) class I chain loci from the opossum Monodelphis domestica was isolated, fully sequenced, and characterized. This sequence represents the largest contiguous genomic sequence reported for the MHC region of a nonplacental mammal. Based on particular conserved amino acid residues, and limited expression analyses, the two MHC-I loci, designated ModoUB and ModoUC, appear to encode functional MHC-I molecules. The two coding regions are 98% identical at the nucleotide level; however, their promoter regions differ significantly. Two CpG islands present in the cosmid sequence correspond to the two coding regions. Twelve microsatellites and six retroelements were also present in the cosmid. The retroelements share highest sequence homology to the CORE–SINE family of retroelements. Due to high sequence identity, it is very likely that ModoUB and ModoUC loci are products of recent gene duplication that occurred less than 4 million years ago. 相似文献
132.
A multivariate linear regression model is proposed for predicting and mapping rare vascular plant species richness in Finnish agricultural landscapes according to landscape variables. The data used in developing the model were derived from a floristic inventory from 105 0.5 km × 0.5 km grid squares. Using a stepwise multiple regression technique, four landscape variables were found to explain 71.8% of the variability in the number of rare plant species. The results suggest that the local `hotspots' of rare plants (squares with 5 rare taxa) are mainly found in heterogeneous river valleys, where extensive semi-natural grasslands and herb-rich forests occur on the steep slopes. According to other similar studies, intermediate human disturbance increases the number of rare species in agricultural landscapes. It appears that empirical models based on landscape variables derived from digital maps can provide relatively accurate surrogates for extensive field surveys and fine-scale observations on the distributions of rare taxa in agricultural landscapes. Potential reasons for the performance of the model and the ecology and habitats of the species concerned are discussed. 相似文献
133.
134.
Jonathan Lenoir Risto Virtanen Jari Oksanen Lauri Oksanen Miska Luoto John‐Arvid Grytnes Jens‐Christian Svenning 《Global Ecology and Biogeography》2012,21(8):851-860
Aim The role of dispersal in structuring biodiversity across spatial scales is controversial. If dispersal controls regional and local community assembly, it should also affect the degree of spatial species turnover as well as the extent to which regional communities are represented in local communities. Here we provide the first integrated assessment of relationships between dispersal ability and local‐to‐regional spatial aspects of species diversity across a large geographical area. Location Northern Eurasia. Methods Using a cross‐scale analysis covering local (0.64 m2) to continental (the Eurasian Arctic biome) scales, we compared slope parameters of the dissimilarity‐to‐distance relationship in species composition and the local‐to‐regional relationship in species richness among three plant‐like groups that differ in dispersal ability: lichens with the highest dispersal ability; mosses and moss allies with intermediate dispersal ability; and seed plants with the lowest dispersal ability. Results Diversity patterns generally differed between the three groups according to their dispersal ability, even after controlling for niche‐based processes. Increasing dispersal ability is linked to decreasing spatial species turnover and an increasing ratio of local to regional species richness. All comparisons supported our expectations, except for the slope of the local‐to‐regional relationship in species richness for mosses and moss allies which was not significantly steeper than that of seed plants. Main conclusions The negative link between dispersal ability and spatial species turnover and the corresponding positive link between dispersal ability and the ratio of local‐to‐regional species richness support the idea that dispersal affects community structure and diversity patterns across spatial scales. 相似文献
135.
LM Kamminga JC van Wolfswinkel MJ Luteijn LJ Kaaij MP Bagijn A Sapetschnig EA Miska E Berezikov RF Ketting 《PLoS genetics》2012,8(7):e1002702
RNA interference (RNAi)-related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part regulated by Hen1-mediated methylation of their 3' ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA-methyl-transferase homolog, HENN-1, on the different RNAi pathways in this nematode. We reveal differential effects of HENN-1 on the two pathways that are known to employ methylated sRNA molecules: the 26G and 21U pathways. Surprisingly, in the germline, stability of 21U RNAs, the C. elegans piRNAs, is only mildly affected by loss of methylation; and introduction of artificial 21U target RNA does not further destabilize non-methylated 21U RNAs. In contrast, most 26G RNAs display reduced stability and respond to loss of HENN-1 by displaying increased 3'-uridylation frequencies. Within the 26G RNA class, we find that specifically ERGO-1-bound 26G RNAs are modified by HENN-1, while ALG-3/ALG-4-bound 26G RNAs are not. Global gene expression analysis of henn-1 mutants reveals mild effects, including down-regulation of many germline-expressed genes. Our data suggest that, apart from direct effects of reduced 26G RNA levels of henn-1 on gene expression, most effects on global gene expression are indirect. These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymes in these pathways. 相似文献
136.
Maria Sporbert Helge Bruelheide Gunnar Seidler Petr Keil Ute Jandt Gunnar Austrheim Idoia Biurrun Juan Antonio Campos Andra arni Milan Chytrý Jnos Csiky Els De Bie Jürgen Dengler Valentin Golub John‐Arvid Grytnes Adrian Indreica Florian Jansen Martin Jirouek Jonathan Lenoir Miska Luoto Corrado Marcen Jesper Erenskjold Moeslund Aaron Prez‐Haase Solvita Rsia Vigdis Vandvik Kiril Vassilev Erik Welk 《植被学杂志》2019,30(4):620-632
137.
138.
Gouin N Deakin JE Miska KB Miller RD Kammerer CM Graves JA VandeBerg JL Samollow PB 《Cytogenetic and genome research》2006,112(3-4):277-285
We used genetic linkage mapping and fluorescence in situ hybridization (FISH) to conduct the first analysis of genic organization and chromosome localization of the major histocompatibility complex (MHC) of a marsupial, the gray, short-tailed opossum Monodelphis domestica. Family based linkage analyses of two M. domestica MHC Class I genes (UA1, UG) and three MHC Class II genes (DAB, DMA, and DMB) revealed that these genes were tightly linked and positioned in the central region of linkage group 3 (LG3). This cluster of MHC genes was physically mapped to the centromeric region of chromosome 2q by FISH using a BAC clone containing the UA1 gene. An interesting finding from the linkage analyses is that sex-specific recombination rates were virtually identical within the MHC region. This stands in stark contrast to the genome-wide situation, wherein males exhibit approximately twice as much recombination as females, and could have evolutionary implications for maintaining equality between males and females in the ability to generate haplotype diversity in this region. These analyses also showed that three non-MHC genes that flank the MHC region on human chromosome 6, myelin oligodendrocyte glycoprotein (MOG), bone morphogenetic protein 6 (BMP6), and prolactin (PRL), are split among two separate linkage groups (chromosomes) in M. domestica. Comparative analysis with eight other vertebrate species suggests strong conservation of the BMP6-PRL synteny among birds and mammals, although the BMP6-PRL-MHC-ME1 synteny is not conserved. 相似文献
139.
Katja M. Raatikainen Risto K. Heikkinen Miska Luoto 《Biodiversity and Conservation》2009,18(4):1067-1085
Using species and environmental data from an extensive grassland area in south-western Finland, we investigated the effect
of patch area and connectivity, management and local habitat variables on the occurrence of spring-flowering vascular plants
and their richness in boreal agricultural landscapes. Generalized linear models (GLM) and variation partitioning were used
to study the explanatory power of the three groups of variables and their combined contributions on the richness and occurrence
of six spring-flowering plant species. Generalized additive models (GAMs) and associated cross-validation tests were used
to evaluate the predictability of the species occurrence and richness patterns. Present-day grassland patch area and connectivity
were important predictors for occurrence and richness of the studied plant species. In addition, local habitat factors, especially
radiation, accounted for major fractions of occurrence patterns of the studied species. Hybrid models including variables
from all three variable groups had higher explanatory power and predictive capability than partial models. However, performance
of the separate single-species models varied considerably between the six study species. Exclusion of radiation or connectivity
from the hybrid models decreased their predictive performance, suggesting that these factors are of particular importance
for grassland plant species at their northern range margins. When developing conservation and management planning for grassland
plant species in Northern Europe, attention should be paid to well-connected networks of grassland patches including large,
steeply-sloped patches with a favorable microclimate. 相似文献
140.
The let-7 MicroRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans 总被引:3,自引:0,他引:3
Abbott AL Alvarez-Saavedra E Miska EA Lau NC Bartel DP Horvitz HR Ambros V 《Developmental cell》2005,9(3):403-414
The microRNA let-7 is a critical regulator of developmental timing events at the larval-to-adult transition in C. elegans. Recently, microRNAs with sequence similarity to let-7 have been identified. We find that doubly mutant animals lacking the let-7 family microRNA genes mir-48 and mir-84 exhibit retarded molting behavior and retarded adult gene expression in the hypodermis. Triply mutant animals lacking mir-48, mir-84, and mir-241 exhibit repetition of L2-stage events in addition to retarded adult-stage events. mir-48, mir-84, and mir-241 function together to control the L2-to-L3 transition, likely by base pairing to complementary sites in the hbl-1 3' UTR and downregulating hbl-1 activity. Genetic analysis indicates that mir-48, mir-84, and mir-241 specify the timing of the L2-to-L3 transition in parallel to the heterochronic genes lin-28 and lin-46. These results indicate that let-7 family microRNAs function in combination to affect both early and late developmental timing decisions. 相似文献