全文获取类型
收费全文 | 110篇 |
免费 | 5篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2019年 | 1篇 |
2017年 | 1篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 3篇 |
2012年 | 12篇 |
2011年 | 6篇 |
2010年 | 3篇 |
2009年 | 2篇 |
2008年 | 8篇 |
2007年 | 11篇 |
2006年 | 9篇 |
2005年 | 6篇 |
2004年 | 9篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
1965年 | 2篇 |
1964年 | 1篇 |
1962年 | 2篇 |
1959年 | 1篇 |
1958年 | 3篇 |
1957年 | 1篇 |
1955年 | 3篇 |
1946年 | 1篇 |
1937年 | 1篇 |
排序方式: 共有115条查询结果,搜索用时 15 毫秒
31.
32.
33.
Mischa Titiev 《American anthropologist》1962,64(2):379-380
34.
Helena Jambor Alberto Antonietti Bradly Alicea Tracy L. Audisio Susann Auer Vivek Bhardwaj Steven J. Burgess Iuliia Ferling Magorzata Anna Gazda Luke H. Hoeppner Vinodh Ilangovan Hung Lo Mischa Olson Salem Yousef Mohamed Sarvenaz Sarabipour Aalok Varma Kaivalya Walavalkar Erin M. Wissink Tracey L. Weissgerber 《PLoS biology》2021,19(3)
35.
Xiyuan Bai Nicole E. Feldman Kathryn Chmura Alida R. Ovrutsky Wen-Lin Su Laura Griffin Dohun Pyeon Mischa T. McGibney Matthew J. Strand Mari Numata Seiji Murakami Loretta Gaido Jennifer R. Honda William H. Kinney Rebecca E. Oberley-Deegan Dennis R. Voelker Diane J. Ordway Edward D. Chan 《PloS one》2013,8(4)
36.
Dustin E. Bosch Adam J. Kimple Alyssa J. Manning Robin E. Muller Francis S. Willard Mischa Machius Stephen L. Rogers David P. Siderovski 《Structure (London, England : 1993)》2013,21(1):65-75
- Download : Download high-res image (298KB)
- Download : Download full-size image
37.
Machius M Brautigam CA Tomchick DR Ward P Otwinowski Z Blevins JS Deka RK Norgard MV 《Journal of molecular biology》2007,373(3):681-694
Tp0655 of Treponema pallidum, the causative agent of syphilis, is predicted to be a 40 kDa membrane lipoprotein. Previous sequence analysis of Tp0655 noted its homology to polyamine-binding proteins of the bacterial PotD family, which serve as periplasmic ligand-binding proteins of ATP-binding-cassette (ABC) transport systems. Here, the 1.8 A crystal structure of Tp0655 demonstrated structural homology to Escherichia coli PotD and PotF. The latter two proteins preferentially bind spermidine and putrescine, respectively. All of these proteins contain two domains that sandwich the ligand between them. The ligand-binding site of Tp0655 can be occupied by 2-(N-morpholino)ethanesulfanoic acid, a component of the crystallization medium. To discern the polyamine binding preferences of Tp0655, the protein was subjected to isothermal titration calorimetric experiments. The titrations established that Tp0655 binds polyamines avidly, with a marked preference for putrescine (Kd=10 nM) over spermidine (Kd=430 nM), but the related compounds cadaverine and spermine did not bind. Structural comparisons and structure-based sequence analyses provide insights into how polyamine-binding proteins recognize their ligands. In particular, these comparisons allow the derivation of rules that may be used to predict the function of other members of the PotD family. The sequential, structural, and functional homology of Tp0655 to PotD and PotF prompt the conclusion that the former likely is the polyamine-binding component of an ABC-type polyamine transport system in T. pallidum. We thus rename Tp0655 as TpPotD. The ramifications of TpPotD as a polyamine-binding protein to the parasitic strategy of T. pallidum are discussed. 相似文献
38.
Deka RK Brautigam CA Tomson FL Lumpkins SB Tomchick DR Machius M Norgard MV 《The Journal of biological chemistry》2007,282(8):5944-5958
The Tp34 (TP0971) membrane lipoprotein of Treponema pallidum, an obligate human pathogen and the agent of syphilis, was previously reported to have lactoferrin binding properties. Given the non-cultivatable nature of T. pallidum, a structure-to-function approach was pursued to clarify further potential relationships between the Tp34 structural and biochemical properties and its propensity to bind human lactoferrin. The crystal structure of a nonacylated, recombinant form of Tp34 (rTp34), solved to a resolution of 1.9A(,) revealed two metaloccupied binding sites within a dimer; the identity of the ion most likely was zinc. Residues from both of the monomers contributed to the interfacial metal-binding sites; a novel feature was that the delta-sulfur of methionine coordinated the zinc ion. Analytical ultracentrifugation showed that, in solution, rTp34 formed a metal-stabilized dimer and that rTp34 bound human lactoferrin with a stoichiometry of 2:1. Isothermal titration calorimetry further revealed that rTp34 bound human lactoferrin at high (submicromolar) affinity. Finally, membrane topology studies revealed that native Tp34 is not located on the outer surface (outer membrane) of T. pallidum but, rather, is periplasmic. How propensity of Tp34 to bind zinc and the iron-sequestering lactoferrin may relate overall to the biology of T. pallidum infection in humans is discussed. 相似文献
39.
RIM proteins play critical roles in synaptic vesicle priming and diverse forms of presynaptic plasticity. The C-terminal C2B domain is the only module that is common to all RIMs but is only distantly related to well-studied C2 domains, and its three-dimensional structure and interactions have not been characterized in detail. Using NMR spectroscopy, we now show that N- and C-terminal extensions beyond the predicted C2B domain core sequence are necessary to form a folded, stable RIM1alpha C2B domain. We also find that the isolated RIM1alpha C2B domain is not sufficient for previously described protein-protein interactions involving the RIM1alpha C-terminus, suggesting that additional sequences adjacent to the C2B domain might be required for these interactions. However, analytical ultracentrifugation shows that the RIM1alpha C2B domain forms weak dimers in solution. The crystal structure of the RIM1alpha C2B domain dimer at 1.7 A resolution reveals that it forms a beta-sandwich characteristic of C2 domains and that the unique N- and C-terminal extensions form a small subdomain that packs against the beta-sandwich and mediates dimerization. Our results provide a structural basis to understand the function of RIM C2B domains and suggest that dimerization may be a crucial aspect of RIM function. 相似文献
40.