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151.
Etsuko Mori Jerry L Hedrick Nate J Wardrip Tsuneatsu Mori Seiichi Takasaki 《Glycoconjugate journal》1998,15(5):447-456
Structures of acidic N-glycans released from porcine zona pellucida glycoproteins by hydrazinolysis were studied. The results indicated that the acidic glycans are of mono- to tetraantennary complex-type with and without N-acetyllactosamine repeating units. Sulfated residues are not only located at the C-6 position of GlcNAc included in the N-acetyllactosamine repeating units, but also at the C-6 position of GlcNAc in the non-repeated antennae and at the C-3 position of reducing terminal GlcNAc residue. Analysis of the oligosaccharide fragments released by endo--galactosidase digestion and by hydrazine/nitrous acid treatment also revealed that various sulfated and non-sulfated forms of fucosylated structures such as Fuc12Gal14(±SO–36)GlcNAc (type 2H), Gal14(Fuc13)(±SO–36)GlcNAc(Lex) and Fuc13 or 4(±SO–36)GlcNAc, are expressed in the repeated outer chain moieties. 相似文献
152.
Yoh Kodera Katsukiyo Sakurai Yuiko Satoh Takeshi Uemura Yuji Kaneda Hiroyuki Nishimura Misao Hiroto Ayako Matsushima Yuji Inada 《Biotechnology letters》1998,20(2):177-180
Lipase modified with polyethylene glycol became soluble and active in organic solvents, and catalyzed regioselective deacetylation of peracetylated monosaccharide derivatives in 1,1,1-trichloroethane. The deacetylation occurred only at the positions of C-4 and C-6 of the glycopyranoside ring. Especially, peracetylated methyl -D-xylopyranoside and peracetylated L-serine--D-xylopyranoside were hydrolyzed only at the position of C-4. Subsequently, one of the resulting products, that is L-serine-2,3-di-O-acetyl--D-xylopyranoside, was coupled with galactose residue to obtain L-serine-4-O-(-D-galactopyranosyl)--D-xylopyranoside, a model compound of the carbohydrate-protein linkage region of proteoglycans. 相似文献
153.
154.
Shigeo Uehara Toshisada Nishida Hiroyuki Takasaki Mahale Mountains Wildlife Kohshi Norikoshi Takahiro Tsukahara Ramadhani Nyundo Miya Hamai 《Primates; journal of primatology》1994,35(3):275-281
K Group, originally one of the two major study groups of chimpanzees since 1965 in the Mahale Mountains National Park, western
Tanzania, was almost extinct by 1983: at most seven individuals remained in the group at the beginning of 1983. K Group continued
to exist for more than four years, but in 1987 a male was left alone at the age of 15 after all the other chimpanzees of the
group emigrated or disappeared. Since then he has been observed sporadically for more than five years only within the former
range of K Group, without having any contact with the many resident chimpanzees of the neighboring M Group, the other major
study group. The present observations reconfirm the strong philopatric tendency of adult male chimpanzees. 相似文献
155.
Hiroshi Takemoto Shinji Nishimura Yumi Kosada Satoshi Hata Shin Takagi Susumu Hosoi Kiyoshi Ezumi Misao Ide Shigenori Harada 《Microbiology and immunology》1994,38(1):63-71
Anti-human IgE monoclonal antibodies (mAbs) were produced and eight clones recognizing epitopes on native IgE were selected. Epitopes were mapped by a competitive inhibition enzyme-linked immunosorbent assay, Western blotting and a multi-pin peptide technology. Four sites (one each in the Cε1, Cε2, Cε2/Cε3 junction and Cε3) were recognized by the mAbs. The relationship between the four epitopes and the binding sites of high and low affinity IgE receptors (FcεRI and FcεRII, respectively) was studied using a monovalent Fab fragment of each mAb as a binding inhibitor. The IgE-FcεRII binding was clearly inhibited by the mAb recognizing the Cε2/Cε3 junction, suggesting that FcεRII binds to a rather limited area around the Cε2/Cε3 junction. The IgE-FcεRI binding, on the other hand, was scarcely inhibited by any single mAb. However, the binding was inhibited when the epitope in Cε2 was blocked simultaneously with that at the Cε2/Cε3 junction or with that in Cε3, indicating that these three distinct epitopes are related to the FcεRI binding sites. When these three epitopes were shown in the stereograph of human IgE, the FcεRI binding area was spread largely on the groove side between Cε2 and Cε3 domains. These results suggest that FcεRI acquires the high affinity through multiple bindings. 相似文献
156.
Development of medium components for the production of glucosyl-transferring enzyme by Aureobasidium
S. Hayashi T. Hinotani T. Hayashi Y. Takasaki K. Imada 《World journal of microbiology & biotechnology》1993,9(2):248-250
Aureobasidium sp. ATCC 20524 produced a glucosyl-transferring enzyme which produced panose (O--D-glucopyranosyl-(1»6)-O--D-glucopyranosyl-(1»4)-d-glucose) from maltose. Optimum production for the enzyme was with maltose at 2% (w/v) and yeast extract at 1.5% (w/v). Enzymatic activity reached 0.7×103 U/g dry cells after 48 h. 相似文献
157.
Koichi Koshimizu Hajime Ohigashi Michael A. Huffman Toshisada Nishida Hiroyuki Takasaki 《International journal of primatology》1993,14(2):345-356
Potential medicinal plants for wild chimpanzees have been studied in order to discover their physiologically active compounds. Tests of the physiological activity of 3 plant species—Vernonia amygdalina, Aspilia mossambicensis, andFicus exasperata—indicate that they contain a variety of active compounds. From one species,V. amygdalina, an antitumor agent and 2 possible antitumor promoters are identified. Furthermore, steroid glucosides were isolated as the bitter substances. These structurally new compounds are expected to exhibit a number of significant physiological activities. The chemical investigation of possible medicinal plants used by chimpanzees should be helpful in recovering naturally occurring compounds of medicinal significance for human use. 相似文献
158.
S. Hayashi K. Matsumoto Y. Wada Y. Takasaki K. Imada 《Letters in applied microbiology》1993,17(2):75-77
A crude extract from Aureobasidium had β-glucosidase activity, hydrolysing cello-biose, methyl-β-D-glucoside, lactose, carboxymethylcellulose, avicel, o -nitrophenyl-β-D-glucoside and p -nitrophenyl-β-D-glucoside, and had favourable properties such as high pH and thermal stabilities. The optimum pH and temperature of the cello-biase activity were 4 and 80°C, respectively. The cellobiase activity was stable at pH 3–7 to 7.8 for at least 3 h, and retained 34 and 78% of its original activity at pH 1.5 and 9, respectively. Cellobiase activity was stable at 80°C for 15 min, and retained 81% of its original activity at 85°C. 相似文献
159.
Osamu Takenaka Sakie Kawamoto Toshifumi Udono Minori Arakawa Hiroyuki Takasaki Akiko Takenaka 《Primates; journal of primatology》1993,34(3):357-363
Previously designed primers for the polymerase chain reaction (PCR) amplifying microsatellite DNA segments containing GT/AC
dinucleotide repeats in the chimpanzee (Pan troglodytes) genome were used for paternity testing in a breeding colony in captivity. Combinations of three PCR primers identified the
fathers of all the tested 40 chimpanzees born in an eight-year period. The results suggested: (1) a positive (though not conclusive)
correlation between male rank and number of offspring; (2) choice of mating partners by the female rather than by the male;
and (3) absence of stable mating pairs over the years. For studies of chimpanzees in captivity and in the wild, these primers
should be useful for paternity testing, for investigating genetic variations, and for improving genetic maintenance of breeding
colonies. 相似文献
160.
Fumiya Obata Misao Tsunoda Takehisa Kaneko Koichi Ito Ichiro Ito Susan Masewicz Eric M. Mickelson William E. R. Ollier Graham Pawelec Marina Cella Giovanni B. Ferrara Noboru Kashiwagi 《Immunogenetics》1993,38(1):67-70
Correspondence to: F. Obata. 相似文献