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41.
Mirza D. Kusrini Jodi J. L. Rowley Luna R. Khairunnisa Glenn M. Shea Ronald Altig 《PloS one》2015,10(1)
Most of the reproductive modes of frogs include an exotrophic tadpole, but a number of taxa have some form of endotrophic development that lacks a feeding tadpole stage. The dicroglossid frog genus Limnonectes ranges from China south into Indonesia. The breeding biologies of the approximately 60 described species display an unusual diversity that range from exotrophic tadpoles to endotrophic development in terrestrial nests. There have been mentions of oviductal production of typical, exotrophic tadpoles in an undescribed species of Limnonectes from Sulawesi, Indonesia. Here we examine newly collected specimens of this species, now described as L. larvaepartus and present the first substantial report on this unique breeding mode. Typical exotrophic tadpoles that are retained to an advanced developmental stage in the oviducts of a female frog are birthed into slow-flowing streams or small, non-flowing pools adjacent to the streams. 相似文献
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43.
Md Iqbal Kabir Md Bayzidur Rahman Wayne Smith Mirza Afreen Fatima Lusha Abul Hasnat Milton 《PloS one》2015,10(8)
Background
Bangladesh is one of the most vulnerable countries to climate change. People are getting educated at different levels on how to deal with potential impacts. One such educational mode was the preparation of a school manual, for high school students on climate change and health protection endorsed by the National Curriculum and Textbook Board, which is based on a 2008 World Health Organization manual. The objective of this study was to test the effectiveness of the manual in increasing the knowledge level of the school children about climate change and health adaptation.Methods
This cluster randomized intervention trial involved 60 schools throughout Bangladesh, with 3293 secondary school students participating. School upazilas (sub-districts) were randomised into intervention and control groups, and two schools from each upazila were randomly selected. All year seven students from both groups of schools sat for a pre-test of 30 short questions of binary response. A total of 1515 students from 30 intervention schools received the intervention through classroom training based on the school manual and 1778 students of the 30 control schools did not get the manual but a leaflet on climate change and health issues. Six months later, a post-intervention test of the same questionnaire used in the pre-test was performed at both intervention and control schools. The pre and post test scores were analysed along with the demographic data by using random effects model.Results
None of the various school level and student level variables were significantly different between the control and intervention group. However, the intervention group had a 17.42% (95% CI: 14.45 to 20.38, P = <0.001) higher score in the post-test after adjusting for pre-test score and other covariates in a multi-level linear regression model.Conclusions
These results suggest that school-based intervention for climate change and health adaptation is effective for increasing the knowledge level of school children on this topic. 相似文献44.
Adnan Massadeh Ahmed Gharibeh Khaled Omari Idrees Al-Momani Ahmed Alomari Hiatham Tumah Wail Hayajneh 《Biological trace element research》2010,133(1):1-11
A total of 73 blood samples (56 from smokers and 17 from nonsmokers) were collected to determine the concentrations of selected
heavy metal in the whole blood of smokers and nonsmokers living in and around the city of Amman, Jordan. Analysis of heavy
metals in the whole blood samples of various groups took in consideration the number of cigarettes smoked per day. The analysis
of blood samples was carried out using inductively coupled plasma optical emission spectrometry. This study aimed to evaluate
the blood metal levels in smokers and nonsmokers and to assess the influence of smoking cigarettes on blood metal levels.
The results were compared with those from a control group. The results indicated that the average concentrations of cadmium
(Cd), lead (Pb), copper (Cu), zinc (Zn), and selenium (Se) were 0.0313, 0.344, 2.328, 3.214, and 0.332 mg/L, respectively.
Statistical analysis of results indicated that these average concentrations were significantly higher compared with the average
concentrations in nonsmokers (P < 0.05). Moreover, the correlations between blood metal and other blood metal levels in smokers, the correlations between
blood metal and other blood metal levels in nonsmokers, and the correlations between blood metal concentration in smokers
and its concentration in nonsmokers were calculated. The standard reference material (blood serum National Institute of Standards
and Technology 1598) and the quality control were used to validate the reliability of the method used for the estimation of
heavy metals in blood samples. Results revealed that there was an agreement between the certified values and the measured
values. 相似文献
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46.
Tim R. B. Davenport Daniela W. De Luca Claire E. Bracebridge Sophy J. Machaga Noah E. Mpunga Omari Kibure Yahya S. Abeid 《Primates; journal of primatology》2010,51(3):213-220
The diet and feeding behaviour of the kipunji (Rungwecebus kipunji) was studied over 45 months, the first dietary analysis for this species. During 9498 h of direct observation of 34 kipunji
groups, a list of 122 identified foodplants was recorded. The list represents 60 families, including 64 trees, 30 herbs, 9
climbers, 7 shrubs, 6 lianas, 3 grasses and 3 ferns. Kipunji were observed eating bark, young and mature leaves, ripe and
unripe fruits, flowers, pith, seed pods, rhizomes, tubers, shoots and stalks. Invertebrates, fungi, moss, lichen, and soil
were also eaten. Macaranga capensis var. capensis, an early successional tree, was the most commonly consumed species, with leaves, leaf stalks, pith, flowers and bark all
eaten. We demonstrate that the kipunji is an omnivorous dietary generalist, favouring mature and immature leaves, ripe and
unripe fruits and bark in similar proportions, with an almost comparable fondness for leaf stalks and flowers. Kipunji appear
to be adaptable foragers able to modify their diet seasonally, being more folivorous in the dry season and more frugivorous
in the wet. Whereas more ripe fruit is eaten in the wet season, the proportion of unripe fruit remains similar across the
year. The proportion of mature leaves and pith increases throughout the dry season at the expense of ripe fruits and bark,
and this may compensate nutritionally for the lack of available dry-season ripe fruits. Relatively more pith is eaten in the
dry season, more stalks at the end of the dry and beginning of the wet seasons, and bark consumption increases as the rainfall
rises. 相似文献
47.
Denise S. H. Tan Yuchen Ang Gwynne S. Lim Mirza Rifqi Bin Ismail Rudolf Meier 《Zoologica scripta》2010,39(1):51-61
Tan, D. S. H., Ang, Y., Lim, G. S., Ismail, M. R. B. & Meier, R. (2010). From ‘cryptic species’ to integrative taxonomy: an iterative process involving DNA sequences, morphology, and behaviour leads to the resurrection of Sepsis pyrrhosoma (Sepsidae: Diptera). —Zoologica Scripta, 39, 51–61. The increased availability of DNA sequences has led to a surge of ‘cryptic species’ in the literature. These units are usually proposed based on finding genetically distinct lineages within species that were initially defined based on morphological characters. However, few authors attempt to confirm whether these ‘cryptic’ units are species and even fewer authors are explicit about which species concept is applied. Here, we use an example from Sepsidae (Diptera) to demonstrate how cryptic species can be validated by an iterative process involving several data sources and an evaluation of the data under different species concepts. A phylogeographic analysis based on 50 specimens for five species of the flavimana group revealed deep mitochondrial splits within Sepsis flavimana which was suggestive of a cryptic species. We resolve the initial conflict between DNA sequences and morphology by adding new morphological data as well as behavioural evidence and tests for reproductive isolation. One cryptic species is confirmed and Sepsis pyrrhosoma, a former synonym of S. flavimana, is here shown to be a valid species under most species concepts. We can thus document that the same data can lead to similar conclusions under conflicting concepts once different kinds of data are integrated. 相似文献
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49.
Senkovich O Cook WJ Mirza S Hollingshead SK Protasevich II Briles DE Chattopadhyay D 《Journal of molecular biology》2007,370(4):701-713
Human lactoferrin, a component of the innate immune system, kills a wide variety of microorganisms including the Gram positive bacteria Streptococcus pneumoniae. Pneumococcal surface protein A (PspA) efficiently inhibits this bactericidal action. The crystal structure of a complex of the lactoferrin-binding domain of PspA with the N-lobe of human lactoferrin reveals direct and specific interactions between the negatively charged surface of PspA helices and the highly cationic lactoferricin moiety of lactoferrin. Binding of PspA blocks surface accessibility of this bactericidal peptide preventing it from penetrating the bacterial membrane. Results of site-directed mutagenesis, in vitro protein binding assays and isothermal titration calorimetry measurements corroborate that the specific electrostatic interactions observed in the crystal structure represent major associations between PspA and lactoferrin. The structure provides a snapshot of the protective mechanism utilized by pathogens against the host's first line of defense. PspA represents a major virulence factor and a promising vaccine candidate. Insights from the structure of the complex have implications for designing therapeutic strategies for treatment and prevention of pneumococcal diseases that remain a major public health problem worldwide. 相似文献
50.