Amylase heterogeneity variants in man

The existence of six amylase heterogeneity variants in serum and urine has been discovered in a group of 111 families with twins, and the frequence of each variant was determined. It had been shown that each of these amylase heterogeneity variants is inherited codominantly, and the mode of inheritance is demonstrated schematically.These amylase heterogeneity variants may be used as an aid to twin zygosity determination and also in paternity problems.     

Contribution to the structural characterization of gamma globulin from pig colostrum

From the results obtained in the present work it is concluded that gamma globulin of the 7 S type (γG) which represents the main immunoglobulin component of pig colostrum, differs from serum γG globulin by the presence of another type of polypeptide chain, designed L₂; the latter was detected after S-sulfonation in starch gel electrophoresis as the fastest moving component and its immunoelectrophoretic pattern shows the presence of two precipitating components. Preparation of soluble heavy and light chains enabled us to study them imunochemically. The heavy chain is represented by two zones; the fainter one was not detected in comparative analysis of the heavy chain of serum γG globulin. The L₁ chain of colostral gamma globulin and the light chain of serum gamma globulin seem to contain three precipitating components, one of which appears due to aging of the chain solution in the presence of glycine. The material from colostrum seems to contain a larger amount of this component than serum. Further it was shown that the component arising in this way is antigenically closely related to the heavy chain.     

Study of the growth and development ofChlorella populations in the culture as a whole

Colour tests for study of the nuclear apparatus ofChlorella cells are described. It can be concluded from the results thatChlorella cell nucleus contains a relatively large nucleolus; the peripheral layers of the nucleus are rich in chromatin only in the nuclear division phase. The nucleus divides by a simplified form of mitosis, characterized morphologically by simple constriction.     

Changes in the biological activity of agropyrene on combined application with humus substances in the cultivation ofScenedesmus obliquus (TURP.) krüger

The work is concerned with the question of the effect of humic acids on the biological action of agropyrene, the effective substance from the rootstock of Agropyron repens (L.) P. BEAUV. The test organism was the alga Scenedesmus obliquus (TURP.) KRÜGER, cultivated in mineral solution. The basic biological test was the determination of the number and size of the cells of this alga. It was found that the way in which agropyrene acts on combined application with humus acids depends on which humus fraction is used. It can display a synergic action if humic acid is used, since this substance probably facilitates the penetration of agropyrene into the cells. On the other hand, fulvic acid counteracts the activity of agropyrene and this most probably by mutual binding and blocking of active groups and links.     

Die durch Blattdüngung mit Humusstoffen hervorgerufenen anatomischen und physiologischen Veränderungen der Zuckerrübe

V p?edlo?ené práci je sledován ú?inek humusových látek aplikovaných na listy cukrovky post?ikem. Sou?asně je ově?ována vhodnost kombinace humusových látek s minerálními ?ivinami. Ukazuje se, ?e post?ik humusovými látkami zvlá?tě v kombinaci s minerálními ?ivinami p?íznivě ovlivňuje r?st cukrovky, zvy?uje váhu list? i ko?ene a celkové mno?ství cukru v ko?eni. Ú?inok post?iku humusovými látkami je vět?í u rostlin pěstovaných ve vodní kultu?e a st?íkaných ?ivným roztokem s kompletněj?ím zastoupením minerálních prvk?. Humusové látky p?i aplikaci na list vyvolávají podobné změny v anatomické stavbě pletiv a orgán?, jako p?i jejich aplikaci do ?ivného roztoku ke ko?en?m. Humusové látky zvlá?tě v kombinaci s minerálním roztokem zvy?ují v listech cukrovky mno?ství chlorofylu a zvy?ují intezitu fotosyntézy. Post?ik humusovými látkami zvy?uje sou?asně transpiraci cukrovky.     

Euglena gracilis as a supplementary test organism for detecting biologically active compounds

The mutagenic activity of more than 120 antimicrobial agents and protective components was investigated. Only Kathon showed a consistent increase in revertant counts in the Ames test onSalmonella typhimurium. The hereditary bleaching test onEuglena gracilis used for detecting extranuclear mutations, showed positive results for Kathon, triethanolamine and diamine silver tetraborate.