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The arbuscular mycorrhizal status of two plant species, Biscutella laevigata L. and Plantago lanceolata L., was investigated on calamine spoil mounds in Boles^aw (southern Poland). Although B. laevigata is a member of the Brassicaceae, a family generally accepted as non-mycorrhizal, this species formed AM symbioses on both heavy metal-contaminated and non-contaminated sites. Besides vesicles and coils, arbuscules were also observed, especially in roots collected prior to seed maturity. Relative mycorrhizal root length and relative arbuscular richness were usually much higher in P. lanceolata than in B. laevigata but not absolute arbuscule richness. Roots of P. lanceolata showed higher colonisation than B. laevigata. Although roots were collected from plants in close proximity, no correlation in mycorrhizal parameters was found between the two species. 相似文献
993.
Inhibition of chitosan-immobilized urease by slow-binding inhibitors: Ni, F and acetohydroxamic acid
Barbara Krajewska Wies
awa Zaborska Maciej Leszko 《Journal of Molecular Catalysis .B, Enzymatic》2001,14(4-6):101-109
The inhibitions by Ni2+ and F− ions and by acetohydroxamic acid of jack bean urease covalently immobilized on chitosan membrane was studied (pH 7.0, 25°C) and compared with those of the native enzyme. The reaction progress curves of the immobilized urease-catalyzed hydrolysis of urea were recorded in the absence and presence of the inhibitors. They revealed that the inhibitions are of the competitive slow-binding type similar to those of native urease. The immobilization weakened the inhibitory effect of the inhibitors on urease as measured by the inhibition constants Ki*. The increase in their values: 17.9-fold for Ni2+, 26.5-fold for F− and 1.7-fold for acetohydroxamic acid, was accounted for by environmental effects generated by heterogeneity of the urease–chitosan system: (1) mass transfer limitations imposed on substrate and reaction product in the external solution, and (2) the increase in local pH on the membrane produced by both the enzymatic reaction and the electric charge of the support. By relating the KM/Ki* ratio to the electrostatic potential of chitosan it was found that while the reduced Ni2+ inhibition is mainly brought about by the potential, inhibition by acetohydroxamic acid is independent of the potential, and the acid inhibits urease in its non-ionic form. The reduction in F− inhibition was ascribed to the increased pH in the local environment of the immobilized enzyme. 相似文献
994.
Agnieszka Fischer Danuta Wiechuła Lidia Postek-Stefańska Jerzy Kwapuliński 《Biological trace element research》2009,132(1-3):19-26
The study shows the results of research on the concentrations of metals (Cd, Pb, Mn, Cu, Cr, Fe, Zn, Na, K, Mg, and Ca) in deciduous and permanent teeth with respect to their location in the oral cavity (maxilla, mandible). It has been found that the concentrations of metals were significantly higher in the deciduous teeth than in permanent ones. Regression analysis and principal component analysis revealed an increased dynamics of the processes of binding the elements by the hydroxyapatite of deciduous teeth. Higher concentrations of the metals were found in the maxilla permanent and deciduous teeth than in the mandible ones. 相似文献
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