全文获取类型
收费全文 | 270篇 |
免费 | 17篇 |
国内免费 | 2篇 |
出版年
2021年 | 6篇 |
2020年 | 6篇 |
2019年 | 6篇 |
2018年 | 2篇 |
2016年 | 6篇 |
2015年 | 11篇 |
2014年 | 14篇 |
2013年 | 14篇 |
2012年 | 12篇 |
2011年 | 14篇 |
2010年 | 11篇 |
2009年 | 12篇 |
2008年 | 12篇 |
2007年 | 18篇 |
2006年 | 14篇 |
2005年 | 11篇 |
2004年 | 12篇 |
2003年 | 10篇 |
2002年 | 14篇 |
2001年 | 9篇 |
2000年 | 6篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1995年 | 3篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 6篇 |
1970年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1965年 | 1篇 |
1964年 | 1篇 |
1961年 | 1篇 |
1959年 | 1篇 |
1955年 | 1篇 |
1933年 | 1篇 |
排序方式: 共有289条查询结果,搜索用时 93 毫秒
281.
Nancy Costigliola Maryna T. Kapustina Gabriel E. Weinreb Zenon Rajfur Ken Jacobson 《Biophysical journal》2010,99(4):1053-1063
When microtubules are depolymerized in spreading cells, they experience morphological oscillations characterized by a period of about a minute, indicating that normal interactions between the microfilament and microtubule systems have been significantly altered. This experimental system provides a test bed for the development of both fine- and coarse-grained models of complex motile processes, but such models need to be adequately informed by experiment. Using criteria based on Fourier transform analysis, we detect spontaneous oscillations in spreading cells. However, their amplitude and tendency to operate at a single frequency are greatly enhanced by microtubule depolymerization. Knockdown of RhoA and addition of various inhibitors of the downstream effector of RhoA, Rho kinase, block oscillatory behavior. Inhibiting calcium fluxes from endoplasmic reticulum stores and from the extracellular medium does not significantly affect the ability of cells to oscillate, indicating that calcium plays a subordinate regulatory role compared to Rho. We characterized the dynamic structure of the oscillating cell by light, fluorescence, and electron microscopy, showing how oscillating cells are dynamically polarized in terms of their overall morphology, f-actin and phosphorylated myosin light chain distribution, and nuclear position and shape. Not only will these studies guide future experiments, they will also provide a framework for the development of refined mathematical models of the oscillatory process. 相似文献
282.
Mathieu Miron Owen Z Woody Alexandre Marcil Carl Murie Robert Sladek Robert Nadon 《BMC bioinformatics》2006,7(1):333-17
Background
DNA microarrays are popular tools for measuring gene expression of biological samples. This ever increasing popularity is ensuring that a large number of microarray studies are conducted, many of which with data publicly available for mining by other investigators. Under most circumstances, validation of differential expression of genes is performed on a gene to gene basis. Thus, it is not possible to generalize validation results to the remaining majority of non-validated genes or to evaluate the overall quality of these studies. 相似文献283.
284.
285.
286.
287.
288.
Interposition agents are a well-known device for attaching legacy applications to distributed systems. However, agents are difficult to build and are often large, monolithic pieces of software which are suited only to limited applications or systems. We solve this problem with Bypass, a language and a tool for quickly building multiple small agents that can be combined together to create powerful yet manageable software. 相似文献
289.