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991.
Abstract The UNESCO-MAB Programme, and in particular the Biosphere Reserve Network, is described within the context of international strategies aimed at ecological sustainability in cities. An innovative acceptation of Biosphere Reserves for urban areas, based on the landscape ecology principle of the integration of natural, social, economic and cultural knowledge, is proposed. The interdisciplinary definition of structure and functions for Urban Biosphere Reserves envisages: (i) the inclusion of the whole metropolitan territory within the Reserve's boundaries; (ii) specific criteria for the delimitation of core areas and buffer zones in urban and periurban contexts; (iii) special focus on transition area requirements; (iv) the improvement in living conditions and a solution to the conflict between humans and the environment by enhancing and harmonizing the overall natural, economic, social and cultural qualities of cities; and (v) support for sustainable planning strategies on a local scale rather than the imposition of new conservation ties. 相似文献
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Ori Braitbard Janette Bishara-Shieban Hava Glickstein Miriam Kott-Gutkowski Umberto Pace Deborah G Rund Wilfred D Stein 《Proteome science》2006,4(1):14-12
Background
We describe the application of an ELISA-based assay (the Peptidomatrix) that can be used to simultaneously identify and quantitate a number of proteins in biological samples. The biological sample (blood component, biopsy, culture or other) is first lysed to release all the proteins, without any additional separation. The denatured proteins in the sample are then digested in bulk with the desired proteolytic enzyme(s). The peptides in the digest are then assayed by appropriate antibodies, using a competition ELISA protocol. 相似文献994.
Francesc X. Prenafeta-Boldú Naiara Rojo Gorka Gallastegui Miriam Guivernau Marc Viñas Ana Elías 《Biodegradation》2014,25(4):557-568
This study reports the biodegradation of carbon disulfide (CS2) in air biofilters packed with a pelletized mixture of composted manure and sawdust. Experiments were carried out in two lab-scale (1.2 L) biofiltration units. Biofilter B was seeded with activated sludge enriched previously on CS2-degrading biomass under batch conditions, while biofilter A was left as a negative inoculation control. This inoculum was characterized by an acidic pH and sulfate accumulation, and contained Achromobacter xylosoxidans as the main putative CS2 biodegrading bacterium. Biofilter operation start-up was unsuccessfully attempted under xerophilic conditions and significant CS2 elimination was only achieved in biofilter A upon the implementation of an intermittent irrigation regime. Sustained removal efficiencies of 90–100 % at an inlet load of up to 12 g CS2 m?3 h?1 were reached. The CS2 removal in this biofilter was linked to the presence of the chemolithoautotrophic bacterium Thiobacillus thioparus, known among the relatively small number of species with a reported capacity of growing on CS2 as the sole energy source. DGGE molecular profiles confirmed that this microbe had become dominant in biofilter A while it was not detected in samples from biofilter B. Conventional biofilters packed with inexpensive organic materials are suited for the treatment of low-strength CS2 polluted gases (IL <12 g CS2 m?3 h?1), provided that the development of the adequate microorganisms is favored, either upon enrichment or by inoculation. The importance of applying culture-independent techniques for microbial community analysis as a diagnostic tool in the biofiltration of recalcitrant compounds has been highlighted. 相似文献
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Physiological indicators of nitrogen and phosphorus deficiency were used to determine the role of these nutrients in the development and proliferation of Cladophora in the Manawatu River, New Zealand. Nitrogen indicator tests showed that surplus nitrogen was available at all times. During a sustained low flow period when the dissolved reactive phosphorus was between 4–5 mg m–3, phosphorus indicator tests revealed that the Cladophora proliferations were phosphorus deficient. A downstream trend of reduced nitrogen and phosphorus availability was also observed during this period. Sudden high river flows were frequent and had a major influence on the Cladophora biomass. 相似文献