全文获取类型
收费全文 | 3357篇 |
免费 | 244篇 |
国内免费 | 3篇 |
出版年
2023年 | 18篇 |
2022年 | 51篇 |
2021年 | 76篇 |
2020年 | 47篇 |
2019年 | 54篇 |
2018年 | 65篇 |
2017年 | 64篇 |
2016年 | 103篇 |
2015年 | 183篇 |
2014年 | 190篇 |
2013年 | 229篇 |
2012年 | 315篇 |
2011年 | 332篇 |
2010年 | 194篇 |
2009年 | 178篇 |
2008年 | 193篇 |
2007年 | 163篇 |
2006年 | 127篇 |
2005年 | 141篇 |
2004年 | 161篇 |
2003年 | 126篇 |
2002年 | 115篇 |
2001年 | 17篇 |
2000年 | 12篇 |
1999年 | 18篇 |
1998年 | 32篇 |
1997年 | 24篇 |
1996年 | 21篇 |
1995年 | 20篇 |
1994年 | 14篇 |
1993年 | 27篇 |
1992年 | 9篇 |
1991年 | 14篇 |
1990年 | 11篇 |
1988年 | 14篇 |
1986年 | 16篇 |
1985年 | 17篇 |
1984年 | 18篇 |
1983年 | 11篇 |
1982年 | 19篇 |
1981年 | 14篇 |
1980年 | 8篇 |
1979年 | 10篇 |
1978年 | 9篇 |
1977年 | 16篇 |
1976年 | 9篇 |
1975年 | 11篇 |
1974年 | 8篇 |
1973年 | 13篇 |
1972年 | 8篇 |
排序方式: 共有3604条查询结果,搜索用时 15 毫秒
981.
The coevolutionary dynamics of obligate ant social parasite systems--between prudence and antagonism
Brandt M Foitzik S Fischer-Blass B Heinze J 《Biological reviews of the Cambridge Philosophical Society》2005,80(2):251-267
In this synthesis we apply coevolutionary models to the interactions between socially parasitic ants and their hosts. Obligate social parasite systems are ideal models for coevolution, because the close phylogenetic relationship between these parasites and their hosts results in similar evolutionary potentials, thus making mutual adaptations in a stepwise fashion especially likely to occur. The evolutionary dynamics of host-parasite interactions are influenced by a number of parameters, for example the parasite's transmission mode and rate, the genetic structure of host and parasite populations, the antagonists' migration rates, and the degree of mutual specialisation. For the three types of obligate ant social parasites, queen-tolerant and queen-intolerant inquilines and slavemakers, several of these parameters, and thus the evolutionary trajectory, are likely to differ. Because of the fundamental differences in lifestyle between these social parasite systems, coevolution should further select for different traits in the parasites and their hosts. Queen-tolerant inquilines are true parasites that exert a low selection pressure on their host, because of their rarity and the fact that they do not conduct slave raids to replenish their labour force. Due to their high degree of specialisation and the potential for vertical transmission, coevolutionary theory would predict interactions between these workerless parasites and their hosts to become even more benign over time. Queen-intolerant inquilines that kill the host queen during colony take-over are best described as parasitoids, and their reproductive success is limited by the existing worker force of the invaded host nest. These parasites should therefore evolve strategies to best exploit this fixed resource. Slavemaking ants, by contrast, act as parasites only during colony foundation, while their frequent slave raids follow a predator prey dynamic. They often exploit a number of host species at a given site, and theory predicts that their associations are best described in terms of a highly antagonistic coevolutionary arms race. 相似文献
982.
Oligonucleotide-peptide conjugates 1-3 were prepared by sequential addition of the appropriate Boc-protected amino acids, followed by nucleoside phosphoramidites in the same support. These molecules are designed to be used for triplex formation and for the directed assembly of nanomaterials. The structures of the desired oligonucleotide-peptide conjugates were confirmed by mass spectrometry on small oligonucleotide-peptide conjugates, by gel electrophoresis, and by hybridization with complementary oligonucleotides. Oligonucleotides carrying the c-myc peptide were specifically recognized by the anti-c-myc monoclonal antibody. 相似文献
983.
Gustavo?Faúndez Miriam?Troncoso Paola?Navarrete Guillermo?FigueroaEmail author 《BMC microbiology》2004,4(1):19
Background
Salmonella enterica and Campylobacter jejuni are amongst the more prevalent bacterial pathogens that cause foodborne diseases. These microorganisms are common contaminants of poultry and poultry products. This study was aimed to evaluate the antibacterial activity of metallic copper surfaces on these important enteropathogens, and to determine the potential acquisition of copper by food exposed to this metal. 相似文献984.
Finlay A McAlister Malcolm Man-Son-Hing Sharon E Straus William A Ghali Paul Gibson David Anderson Jafna Cox Miriam Fradette 《BMC cardiovascular disorders》2004,4(1):1-9
Background
Surprisingly little information on symptoms of paroxysmal atrial fibrillation is available in scientific literature. Using questionnaires, we have analyzed the symptoms associated with arrhythmia attacks.Methods
One hundred randomly-selected patients with idiopathic paroxysmal atrial fibrillation filled in a structured questionnaire.Results
Psychic stress was the most common factor triggering arrhythmia (54%), followed by physical exertion (42%), tiredness (41%) coffee (25%) and infections (22%). Thirty-four patients cited alcohol, 26 in the form of red wine, 16 as white wine and 26 as spirits. Among these 34, red wine and spirits produced significantly more episodes of arrhythmia than white wine (p = 0.01 and 0.005 respectively). Symptoms during arrhythmia were palpitations while exerting (88%), reduced physical ability (87%), palpitations at rest (86%), shortage of breath during exertion (70%) and anxiety (59%). Significant differences between sexes were noted regarding swollen legs (women 21%, men 6%, p = 0.027), nausea (women 36%, men 13%, p = 0.012) and anxiety (females 79%, males 51%, p = 0.014).Conclusion
Psychic stress was the commonest triggering factor in hospitalized patients with paroxysmal atrial fibrillation. Red wine and spirits were more proarrhythmic than white wine. Symptoms in women in connection with attacks of arrhythmia vary somewhat from those in men. 相似文献985.
An activated human follicle-stimulating hormone (FSH) receptor stimulates FSH-like activity in gonadotropin-deficient transgenic mice 总被引:3,自引:0,他引:3
Haywood M Tymchenko N Spaliviero J Koch A Jimenez M Gromoll J Simoni M Nordhoff V Handelsman DJ Allan CM 《Molecular endocrinology (Baltimore, Md.)》2002,16(11):2582-2591
FSH mediates its testicular actions via a specific Sertoli cell G protein-coupled receptor. We created a novel transgenic model to investigate a mutant human FSH receptor (FSHR(+)) containing a single amino acid substitution (Asp567Gly) equivalent to activating mutations in related glycoprotein hormone receptors. To examine the ligand-independent gonadal actions of FSHR(+), the rat androgen-binding protein gene promoter was used to direct FSHR(+) transgene expression to Sertoli cells of gonadotropin-deficient hypogonadal (hpg) mice. Both normal and hpg mouse testes expressed FSHR(+) mRNA. Testis weights of transgenic FSHR(+) hpg mice were increased approximately 2-fold relative to hpg controls (P < 0.02) and contained mature Sertoli cells and postmeiotic germ cells absent in controls, revealing FSHR(+)-initiated autonomous FSH-like testicular activity. Isolated transgenic Sertoli cells had significantly higher basal ( approximately 2-fold) and FSH-stimulated ( approximately 50%) cAMP levels compared with controls, demonstrating constitutive signaling and cell-surface expression of FSHR(+), respectively. Transgenic FSHR(+) also elevated testosterone production in hpg testes, in the absence of circulating LH (or FSH), and it was not expressed functionally on steroidogenic cells, suggesting a paracrine effect mediated by Sertoli cells. The FSHR(+) response was additive with a maximal testosterone dose on hpg testicular development, demonstrating FSHR(+) activity independent of androgen-specific actions. The FSHR(+) response was male specific as ovarian expression of FSHR(+) had no effect on hpg ovary size. These findings reveal transgenic FSHR(+) stimulated a constitutive FSH-like Sertoli cell response in gonadotropin-deficient testes, and pathways that induced LH-independent testicular steroidogenesis. This novel transgenic paradigm provides a unique approach to investigate the in vivo actions of mutated activating gonadotropin receptors. 相似文献
986.
987.
Miriam L. L. Perilli Fernando Lima Flávio H. G. Rodrigues Sandra M. C. Cavalcanti 《PloS one》2016,11(3)
Large cats feeding habits have been studied through two main methods: scat analysis and the carcasses of prey killed by monitored animals. From November 2001 to April 2004, we studied jaguar predation patterns using GPS telemetry location clusters on a cattle ranch in southern Pantanal. During this period, we recorded 431 carcasses of animals preyed upon by monitored jaguars. Concurrently, we collected 125 jaguar scats opportunistically. We compared the frequencies of prey found through each method. We also compared the prey communities using Bray-Curtis similarity coefficient. These comparisons allowed us to evaluate the use of scat analysis as a means to describe jaguar feeding habits. Both approaches identified prey communities with high similarity (Bray-Curtis coefficient > 70). According to either method, jaguars consume three main prey: cattle (Bos taurus), caiman (Caiman yacare) and peccaries (Tayassu pecari and Pecari tajacu). The two methods did not differ in the frequency of the three main prey over dry and wet seasons or years sampled. Our results show that scat analysis is effective and capable of describing jaguar feeding habits. 相似文献
988.
Miriam F. Bennett Jan Huguenin 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1969,63(4):440-445
Summary The effect of geomagnetic force on the light-withdrawal reflex of earthworms, Lumbricus terrestris, L., was investigated during the autumn of 1968. The reactions of worms kept in the earth's magnetic field were timed beginning at 12:00 and at 20:00, E.S.T. on each of 63 days; the same was done for worms maintained in a field whose intensity was essentially zero. The worms in the earth's field withdrew from light significantly faster at night then at midday. No significant difference between the mean reaction times at 12:00 and those of the evening were found for the animals which lived and were tested in the greatly reduced magnetic field. Therefore, geomagnetism does have some effect on the circadian difference in reaction rates in this species.
Zusammenfassung Die Wirkung des erdmagnetisohen Feldes auf die Lichtreaktion von Lumbricus terrestris wurde untersucht (Herbst 1968). Als Test wurde die Reaktion auf einen Lichtreiz verwendet. Ein Teil der Würmer wurde im natürlichen erdmagnetisehen Feld, ein anderer Teil in einem auf Null kompensierten Feld gehalten. Die Reaktionszeiten auf den Lichtreiz wurden bei beiden Gruppen um 12.00 und um 20.00 Uhr gemessen. Die Reaktionszeiten im natürlichen erdmagnetischen Feld waren abends signifikant (p=0,005) kürzer (7,2 sec) als mittags (8,9 sec). Im Feld, das nahezu auf Null kompensiert war, waren die Reaktionszeiten abends und mittags gleich (8,8 bzw. 8,7 sec). Der Erdmagnetismus hat also einen Einfluß auf die circadiane Differenz der Reaktionszeiten bei Lumbricus.相似文献
989.
Portia M. Mira Kristina Crona Devin Greene Juan C. Meza Bernd Sturmfels Miriam Barlow 《PloS one》2015,10(5)
The development of reliable methods for restoring susceptibility after antibiotic resistance arises has proven elusive. A greater understanding of the relationship between antibiotic administration and the evolution of resistance is key to overcoming this challenge. Here we present a data-driven mathematical approach for developing antibiotic treatment plans that can reverse the evolution of antibiotic resistance determinants. We have generated adaptive landscapes for 16 genotypes of the TEM β-lactamase that vary from the wild type genotype “TEM-1” through all combinations of four amino acid substitutions. We determined the growth rate of each genotype when treated with each of 15 β-lactam antibiotics. By using growth rates as a measure of fitness, we computed the probability of each amino acid substitution in each β-lactam treatment using two different models named the Correlated Probability Model (CPM) and the Equal Probability Model (EPM). We then performed an exhaustive search through the 15 treatments for substitution paths leading from each of the 16 genotypes back to the wild type TEM-1. We identified optimized treatment paths that returned the highest probabilities of selecting for reversions of amino acid substitutions and returning TEM to the wild type state. For the CPM model, the optimized probabilities ranged between 0.6 and 1.0. For the EPM model, the optimized probabilities ranged between 0.38 and 1.0. For cyclical CPM treatment plans in which the starting and ending genotype was the wild type, the probabilities were between 0.62 and 0.7. Overall this study shows that there is promise for reversing the evolution of resistance through antibiotic treatment plans. 相似文献
990.