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131.
Towards physiologically meaningful water‐use efficiency estimates from eddy covariance data 下载免费PDF全文
Jürgen Knauer Sönke Zaehle Belinda E. Medlyn Markus Reichstein Christopher A. Williams Mirco Migliavacca Martin G. De Kauwe Christiane Werner Claudia Keitel Pasi Kolari Jean‐Marc Limousin Maj‐Lena Linderson 《Global Change Biology》2018,24(2):694-710
Intrinsic water‐use efficiency (iWUE) characterizes the physiological control on the simultaneous exchange of water and carbon dioxide in terrestrial ecosystems. Knowledge of iWUE is commonly gained from leaf‐level gas exchange measurements, which are inevitably restricted in their spatial and temporal coverage. Flux measurements based on the eddy covariance (EC) technique can overcome these limitations, as they provide continuous and long‐term records of carbon and water fluxes at the ecosystem scale. However, vegetation gas exchange parameters derived from EC data are subject to scale‐dependent and method‐specific uncertainties that compromise their ecophysiological interpretation as well as their comparability among ecosystems and across spatial scales. Here, we use estimates of canopy conductance and gross primary productivity (GPP) derived from EC data to calculate a measure of iWUE (G1, “stomatal slope”) at the ecosystem level at six sites comprising tropical, Mediterranean, temperate, and boreal forests. We assess the following six mechanisms potentially causing discrepancies between leaf and ecosystem‐level estimates of G1: (i) non‐transpirational water fluxes; (ii) aerodynamic conductance; (iii) meteorological deviations between measurement height and canopy surface; (iv) energy balance non‐closure; (v) uncertainties in net ecosystem exchange partitioning; and (vi) physiological within‐canopy gradients. Our results demonstrate that an unclosed energy balance caused the largest uncertainties, in particular if it was associated with erroneous latent heat flux estimates. The effect of aerodynamic conductance on G1 was sufficiently captured with a simple representation. G1 was found to be less sensitive to meteorological deviations between canopy surface and measurement height and, given that data are appropriately filtered, to non‐transpirational water fluxes. Uncertainties in the derived GPP and physiological within‐canopy gradients and their implications for parameter estimates at leaf and ecosystem level are discussed. Our results highlight the importance of adequately considering the sources of uncertainty outlined here when EC‐derived water‐use efficiency is interpreted in an ecophysiological context. 相似文献
132.
Renan Manoel de Oliveira Ana Cristina Schilling Mirco Solé 《Studies on Neotropical Fauna and Environment》2019,54(1):10-21
To coexist, two species should use available resources in different ways. These differences may happen in three different dimensions: temporal, spatial and trophic. In frogs, differences in trophic dimensions are mainly due to different sizes and types of ingested prey. We studied the diet of two syntopic Pithecopus species, addressing prey selectivity and sharing of food resources. Fieldwork was undertaken at the Michelin Ecological Reserve, southern Bahia, between the years 2011 and 2012. Stomach contents were retrieved using a flushing procedure and data on prey availability was obtained. Of the 16 invertebrate categories found in the environment, 11 were consumed by P. rohdei and 12 by P. nordestinus, features that characterize a generalist diet. The most abundant category in the diet of P. nordestinus was Diptera while Hemiptera was the most consumed prey category for P. rohdei. Trophic niche overlap was high considering the size of ingested prey and lower for categories. The selectivity index showed that both species used the resources available in the environment in a slightly different way. The differences found concerning trophic ecology of these two species seem to be sufficient to allow coexistence in the studied area. 相似文献
133.
Robenilson?F.?Santos Ana?Carla?P.?Bitencourt Mirco?Ragni Frederico?V.?Prudente Cecilia?ColettiEmail authorView authors OrcID profile Annalisa?Marzuoli Vincenzo?Aquilanti 《Journal of molecular modeling》2017,23(4):147
The Wigner 9j symbols of the first kind—also known as Fano X-coefficients—serve to connect different addition schemes of four angular momenta, widely known examples being the LS and the jj couplings in atomic, molecular, and nuclear spectroscopies. Here, we also consider alternative sequences of binary couplings of four angular momenta, which are dealt through the 9j symbols of the second kind, and are explicitly given by the pentagonal (or Biedenharn–Elliott) identity. These coefficients are essential ingredients in the quantum-mechanical treatments of rotational and polarization phenomena in reaction dynamics and photoinduced processes. We also emphasize the combinatorial structure underlying the extended construction of a previously introduced truncated icosahedral “abacus”, and provide extensions useful for algebraical manipulations, semiclassical interpretations, and computational applications, including all the 120 addition schemes. 相似文献
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Pulvirenti T Giannotta M Capestrano M Capitani M Pisanu A Polishchuk RS San Pietro E Beznoussenko GV Mironov AA Turacchio G Hsu VW Sallese M Luini A 《Nature cell biology》2008,10(8):912-922
As with other complex cellular functions, intracellular membrane transport involves the coordinated engagement of a series of organelles and machineries; however, the molecular basis of this coordination is unknown. Here we describe a Golgi-based signalling system that is activated by traffic and is involved in monitoring and balancing trafficking rates into and out of the Golgi complex. We provide evidence that the traffic signal is due to protein chaperones that leave the endoplasmic reticulum and reach the Golgi complex where they bind to the KDEL receptor. This initiates a signalling reaction that includes the activation of a Golgi pool of Src kinases and a phosphorylation cascade that in turn activates intra-Golgi trafficking, thereby maintaining the dynamic equilibrium of the Golgi complex. The concepts emerging from this study should help to understand the control circuits that coordinate high-order cellular functions. 相似文献
136.
Lauren M. Hemara Jay Jayaraman Paul W. Sutherland Mirco Montefiori Saadiah Arshed Abhishek Chatterjee Ronan Chen Mark T. Andersen Carl H. Mesarich Otto van der Linden Minsoo Yoon Magan M. Schipper Joel L. Vanneste Cyril Brendolise Matthew D. Templeton 《PLoS pathogens》2022,18(5)
A pandemic isolate of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has devastated kiwifruit orchards growing cultivars of Actinidia chinensis. In contrast, A. arguta (kiwiberry) is not a host of Psa3. Resistance is mediated via effector-triggered immunity, as demonstrated by induction of the hypersensitive response in infected A. arguta leaves, observed by microscopy and quantified by ion-leakage assays. Isolates of Psa3 that cause disease in A. arguta have been isolated and analyzed, revealing a 51 kb deletion in the exchangeable effector locus (EEL). This natural EEL-mutant isolate and strains with synthetic knockouts of the EEL were more virulent in A. arguta plantlets than wild-type Psa3. Screening of a complete library of Psa3 effector knockout strains identified increased growth in planta for knockouts of four effectors–AvrRpm1a, HopF1c, HopZ5a, and the EEL effector HopAW1a –suggesting a resistance response in A. arguta. Hypersensitive response (HR) assays indicate that three of these effectors trigger a host species-specific HR. A Psa3 strain with all four effectors knocked out escaped host recognition, but a cumulative increase in bacterial pathogenicity and virulence was not observed. These avirulence effectors can be used in turn to identify the first cognate resistance genes in Actinidia for breeding durable resistance into future kiwifruit cultivars. 相似文献
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138.
Host organisms have developed sophisticated antiviral responses in order to defeat emerging influenza A viruses (IAVs). At the same time IAVs have evolved immune evasion strategies. The immune system of mammals provides several lines of defence to neutralize invading pathogens or limit their replication. Here, we summarize the mammalian innate and adaptive immune mechanisms involved in host defence against viral infection and review strategies by which IAVs avoid, circumvent or subvert these mechanisms. We highlight well‐characterized, as well as recently described features of this intriguing virus‐host molecular battle. 相似文献
139.
Soil Respiration in European Grasslands in Relation to Climate and Assimilate Supply 总被引:9,自引:0,他引:9
Michael Bahn Mirco Rodeghiero Margaret Anderson-Dunn Sabina Dore Cristina Gimeno Matthias Drösler Michael Williams Christof Ammann Frank Berninger Chris Flechard Stephanie Jones Manuela Balzarolo Suresh Kumar Christian Newesely Tibor Priwitzer Antonio Raschi Rolf Siegwolf Sanna Susiluoto John Tenhunen Georg Wohlfahrt Alexander Cernusca 《Ecosystems》2008,11(8):1352-1367
Soil respiration constitutes the second largest flux of carbon (C) between terrestrial ecosystems and the atmosphere. This
study provides a synthesis of soil respiration (R
s) in 20 European grasslands across a climatic transect, including ten meadows, eight pastures and two unmanaged grasslands.
Maximum rates of R
s (
), R
s at a reference soil temperature (10°C; ) and annual R
s (estimated for 13 sites) ranged from 1.9 to 15.9 μmol CO2 m−2 s−1, 0.3 to 5.5 μmol CO2 m−2 s−1 and 58 to 1988 g C m−2 y−1, respectively. Values obtained for Central European mountain meadows are amongst the highest so far reported for any type
of ecosystem. Across all sites was closely related to . Assimilate supply affected R
s at timescales from daily (but not necessarily diurnal) to annual. Reductions of assimilate supply by removal of aboveground
biomass through grazing and cutting resulted in a rapid and a significant decrease of R
s. Temperature-independent seasonal fluctuations of R
s of an intensively managed pasture were closely related to changes in leaf area index (LAI). Across sites increased with mean annual soil temperature (MAT), LAI and gross primary productivity (GPP), indicating that assimilate supply
overrides potential acclimation to prevailing temperatures. Also annual R
s was closely related to LAI and GPP. Because the latter two parameters were coupled to MAT, temperature was a suitable surrogate
for deriving estimates of annual R
s across the grasslands studied. These findings contribute to our understanding of regional patterns of soil C fluxes and highlight
the importance of assimilate supply for soil CO2 emissions at various timescales. 相似文献
140.
Varga ZT Ramos I Hai R Schmolke M García-Sastre A Fernandez-Sesma A Palese P 《PLoS pathogens》2011,7(6):e1002067
PB1-F2 is a 90 amino acid protein that is expressed from the +1 open reading frame in the PB1 gene of some influenza A viruses and has been shown to contribute to viral pathogenicity. Notably, a serine at position 66 (66S) in PB1-F2 is known to increase virulence compared to an isogenic virus with an asparagine (66N) at this position. Recently, we found that an influenza virus expressing PB1-F2 N66S suppresses interferon (IFN)-stimulated genes in mice. To characterize this phenomenon, we employed several in vitro assays. Overexpression of the A/Puerto Rico/8/1934 (PR8) PB1-F2 protein in 293T cells decreased RIG-I mediated activation of an IFN-β reporter and secretion of IFN as determined by bioassay. Of note, the PB1-F2 N66S protein showed enhanced IFN antagonism activity compared to PB1-F2 wildtype. Similar observations were found in the context of viral infection with a PR8 PB1-F2 N66S virus. To understand the relationship between NS1, a previously described influenza virus protein involved in suppression of IFN synthesis, and PB1-F2, we investigated the induction of IFN when NS1 and PB1-F2 were co-expressed in an in vitro transfection system. In this assay we found that PB1-F2 N66S further reduced IFN induction in the presence of NS1. By inducing the IFN-β reporter at different levels in the signaling cascade, we found that PB1-F2 inhibited IFN production at the level of the mitochondrial antiviral signaling protein (MAVS). Furthermore, immunofluorescence studies revealed that PB1-F2 co-localizes with MAVS. In summary, we have characterized the anti-interferon function of PB1-F2 and we suggest that this activity contributes to the enhanced pathogenicity seen with PB1-F2 N66S- expressing influenza viruses. 相似文献