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Havrylenko S Legouis R Negrutskii B Mirande M 《The Journal of biological chemistry》2011,286(32):28476-28487
MARS is an evolutionary conserved supramolecular assembly of aminoacyl-tRNA synthetases found in eukaryotes. This complex was thought to be ubiquitous in the deuterostome and protostome clades of bilaterians because similar complexes were isolated from arthropods and vertebrates. However, several features of the component enzymes suggested that in the nematode Caenorhabditis elegans, a species grouped with arthropods in modern phylogeny, this complex might not exist, or should display a significantly different structural organization. C. elegans was also taken as a model system to study in a multicellular organism amenable to experimental approaches, the reason for existence of these supramolecular entities. Here, using a proteomic approach, we have characterized the components of MARS in C. elegans. We show that this organism evolved a specific structural organization of this complex, which contains several bona fide components of the MARS complexes known so far, but also displays significant variations. These data highlight molecular evolution events that took place after radiation of bilaterians. Remarkably, it shows that expansion of MARS assembly in metazoans is not linear, but is the result of additions but also of subtractions along evolution. We then undertook an experimental approach, using inactivation of the endogenous copy of methionyl-tRNA synthetase by RNAi and expression of transgenic variants, to understand the role in complex assembly and the in vivo functionality, of the eukaryotic-specific domains appended to aminoacyl-tRNA synthetases. We show that rescue of the worms and assembly of transgenic variants into MARS rest on the presence of these appended domains. 相似文献
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A revision of fish specimens previously identified as Heptapterus mustelinus from the endorheic Río Salí Basin, Tucumán, Argentina, reveals that they present several morphological differences from that species. This paper describes Heptapterus qenqo sp. nov. from the Río Salí Basin. The new species is diagnosed by a combination of the following characters: presence of small serrae on the anterior proximal margin of the first pectoral-fin ray; anal-fin rays iv-v, 11-13 (15-17 total anal-fin rays); adipose-fin base 40·9-47·4% standard length; small eyes (7·4-14·2% head length); adipose-fin confluent to caudal fin and maxillary barbel not reaching pectoral-fin base in adults, and reaching or scarcely surpassing the first pectoral-fin ray in small juveniles. 相似文献
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Myriam Lazard Fabrice Agou Jean Cavarelli Marie-Thrse Latreille Dino Moras Marc Mirande 《Gene》1996,180(1-2):197-205
The genomic organization of the gene encoding rat aspartyl-tRNA synthetase (AspRS), a class II aminoacyl-tRNA synthetase (aaRS), was determined. A single active gene and several pseudogenes were isolated from a rat genomic DNA library and characterized. The active DRS1 gene encoding the rat AspRS spans approximately 60 kb and is divided into 16 exons. Exons 8–16, encoding the nt-binding domain of the synthetase, are clustered in the 3′-region of the gene, whereas exons 3, 4, and 5, encoding the anticodon-binding domain are separated by large introns (up to 15 kb) containing LINE sequences. One of the pseudogenes, ΨDRSI, has a nt sequence 93% identical to that of the complete cDNA sequence of rat AspRS but several stop codons interrupt the coding sequence, thus identifying ΨDRS1 to an inactive processed pseudogene. Two repetitive elements from the LINE family are inserted into ΨDRS1. Calculation of nt substitution rates suggests that ΨDRS1 sequences arose approximately 27 Myr ago. The other pseudogene, ΨDRS2, should be more ancient. Taken together, these results clearly demonstrate that the AspRS gene family is composed of only one active gene. The availability of the gene structure of AspRS could help to clarify molecular evolution of class II aaRS. 相似文献
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Mirande M 《EMBO reports》2007,8(6):547-549
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Moens M Smet A Naudts B Verhoeven J Ieven M Jorens P Geise HJ Blockhuys F 《Letters in applied microbiology》2006,42(2):121-126
AIMS: To evaluate the electronic nose (EN) as method for the identification of ten clinically important micro-organisms. METHODS AND RESULTS: A commercial EN system with a series of ten metal oxide sensors was used to characterize the headspace of the cultured organisms. The measurement procedure was optimized to obtain reproducible results. Artificial neural networks (ANNs) and a k-nearest neighbour (k-NN) algorithm in combination with a feature selection technique were used as pattern recognition tools. Hundred percent correct identification can be achieved by EN technology, provided that sufficient attention is paid to data handling. CONCLUSIONS: Even for a set containing a number of closely related species in addition to four unrelated organisms, an EN is capable of 100% correct identification. SIGNIFICANCE AND IMPACT OF THE STUDY: The time between isolation and identification of the sample can be dramatically reduced to 17 h. 相似文献
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J. Marcos Mirande 《Cladistics : the international journal of the Willi Hennig Society》2009,25(6):574-613
The family Characidae, including more than 1000 species, lacks a phylogenetic diagnosis, with many of its genera currently considered as incertae sedis . The aims of the present study are to propose a phylogenetic diagnosis and to assess higher-level relationships of and within Characidae. In this regard, 360 morphological characters are studied for 160 species of Characidae and related families. Phylogenetic analyses under implied weighting and self-weighted optimization are presented, exploring a broad range of parameters. The analysis under self-weighted optimization is innovative for this size of matrices. Familial status of Serrasalmidae is supported, and Acestrorhynchidae and Cynodontidae are included in a monophyletic Characidae. Engraulisoma taeniatum is transferred from Characidae to Gasteropelecidae. Thus constituted, the monophyly of Characidae is supported by seven synapomorphies. A new subfamily, Heterocharacinae, is proposed, and the subfamilies Aphyocharacinae, Aphyoditeinae, Characinae, Gymnocharacinae, and Stevardiinae are redefined. The Glandulocaudinae are included in Stevardiinae together with remaining members of "clade A" ( sensu Malabarba and Weitzman, 2003 . Comun. Mus. Ciênc. Tecnol. PUCRS, Sér. Zool. 16, 67–151.) and the genera Aulixidens and Nantis . Most incertae sedis genera are assigned, at least tentatively, to a phylogenetically diagnosed clade. 相似文献
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