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71.
R. D. Reeves A. J. M. Baker T. Becquer G. Echevarria Z. J. G. Miranda 《Plant and Soil》2007,293(1-2):107-119
Major collections of the ultramafic flora of Goiás, central Brazil, were made by Brooks and co-workers in 1988 and 1990. At
the time of reports on this material in 1990–1992 much of it had been identified only tentatively and incompletely, but the
area was clearly interesting for taxonomic and biogeochemical reasons. Further progress has been made but still only two-thirds
of the specimens are identified at the species level. Following a third collection in early 2005, we now have 800 specimens
from this area, with chemical analyses of all the plants and of more than 120 representative soil samples. New species have
been found, e.g., in Paspalum (Poaceae) and Pterolepis (Melastomataceae). There is a need for more taxonomic work in genera such as Cnidoscolus (Euphorbiaceae), Lippia (Verbenaceae), Turnera and Piriqueta (Turneraceae), and Vellozia (Velloziaceae). Ni hyperaccumulation (>1,000 mg/kg in dry plant matter) has now been found in a total of 79 specimens, representing
more than 30 different species. Notable Ni hyperaccumulators include Pfaffia sarcophylla (Amaranthaceae), species of Justicia, Lophostachys and Ruellia (Acanthaceae), Porophyllum (Asteraceae), several species of Lippia (Verbenaceae), Turnera and Piriqueta (Turneraceae), and a possibly new Cnidoscolus (Euphorbiaceae). Ni hyperaccumulation has not been found in plants of the outcrops of Morro Feio or Crominia-Mairipotaba;
it seems to be confined to the extensive layered ultramafics of Barro Alto and the Macedo-Niquelandia areas. The distribution
of Ni-values in the Brazilian plant collection is different from that found in the Mediterranean and California, where there
is a clear distinction between accumulator and non-accumulator plants: in Brazil the distribution is more continuous, and
median Ni concentrations are much greater. An ultramafic hill just north of Niquelandia deserves to be protected because of
the presence there of many of the hyperaccumulators and species probably endemic to the Goiás ultramafics. 相似文献
72.
Ribeiro Brbhara Isabella Oenning Braghin Louizi de Souza Magalhes Lansac-Tha Fernando Miranda Bomfim Francieli Ftima Almeida Bia A. Bonecker Cludia Costa Lansac-Tha Fbio Amdeo 《Hydrobiologia》2022,849(14):3135-3147
Hydrobiologia - Beta diversity is the variability in species composition among sampling units for a given area and can be influenced by several environmental drivers, including environmental... 相似文献
73.
74.
Phenotypic and genotypic profile of clinical and animal multidrug‐resistant Salmonella enterica isolates from Mexico 下载免费PDF全文
S. Aguilar‐Montes de Oca M. Talavera‐Rojas E. Soriano‐Vargas J. Barba‐León J. Vázquez‐Navarrete J. Acosta‐Dibarrat C. Salgado‐Miranda 《Journal of applied microbiology》2018,124(1):67-74
Aims
The objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug‐resistant (MDR) isolates from food‐producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico).Methods and Results
A total of 243 isolates were evaluated in terms of antimicrobial resistance (AMR) and related genes through a disk diffusion method and PCR respectively; we found 16 MDR isolates, all of them harbouring the blaCMY gene but not qnr genes, these isolates represent less than 10% of the collection. The pulsed‐field gel electrophoresis revealed a higher genotypic similitude within isolates of State of Mexico than Jalisco.Conclusions
A low percentage of Salmonella isolates were resistant to relevant antibiotics in human health, nevertheless, the AMR and involved genes were similar despite the different serovars and origin of the isolates.Significance and Impact of the Study
This investigation provided an insight of the current status of AMR of Salmonella isolates in two States of Mexico and pinpoint the genes involved in AMR and their epidemiological relationship, the information could help to determine an adequate therapy in human and veterinary medicine. 相似文献75.
Over 250 PDZ (PSD95/Dlg/ZO-1) domain-containing proteins have been described in the human proteome. As many of these possess multiple PDZ domains, the potential combinations of associations with proteins that possess PBMs (PDZ-binding motifs) are vast. However, PDZ domain recognition is a highly specific process, and much less promiscuous than originally thought. Furthermore, a large number of PDZ domain-containing proteins have been linked directly to the control of processes whose loss, or inappropriate activation, contribute to the development of human malignancies. These regulate processes as diverse as cytoskeletal organization, cell polarity, cell proliferation and many signal transduction pathways. In the present review, we discuss how PBM-PDZ recognition and imbalances therein can perturb cellular homoeostasis and ultimately contribute to malignant progression. 相似文献
76.
Leandro Bueno Bergantin Cláudio Fontes Souza Regiane Miranda Ferreira Soraya Soubhi Smaili Neide Hyppolito Jurkiewicz Afonso Caricati-Neto Aron Jurkiewicz 《Cell calcium》2013
It is well established that reduction of Ca2+ influx through L-type voltage-dependent Ca2+ channel (L-type VDCC), or increase of cytosolic cAMP concentration ([cAMP]c), inhibit contractile activity of smooth muscles in response to transmitters released from sympathetic nerves. Surprisingly, in this work we observed that simultaneous administration of L-type VDCC blocker (verapamil) and [cAMP]c enhancers (rolipram, IBMX and forskolin) potentiated purinergic contractions evoked by electrical field stimulation of rat vas deferens, instead of inhibiting them. These results, including its role in sympathetic transmission, can be considered as a “calcium paradox”. On the other hand, this potentiation was prevented by reduction of [cAMP]c by inhibition of adenylyl cyclase (SQ 22536) or depletion of Ca2+ storage of sarco-endoplasmic reticulum by blockade of Ca2+ reuptake (thapsigargin). In addition, cytosolic Ca2+ concentration ([Ca2+]c) evaluated by fluorescence microscopy in rat adrenal medullary slices was significantly reduced by verapamil or rolipram. In contrast, simultaneous incubation of adrenal slices with these compounds significantly increased [Ca2+]c. This effect was prevented by thapsigargin. Thus, a reduction of [Ca2+]c due to blockade of Ca2+ influx through L-type VDCC could stimulate adenylyl cyclase activity increasing [cAMP]c thereby stimulating Ca2+ release from endoplasmic reticulum, resulting in augmented transmitter release in sympathetic nerves and contraction. 相似文献
77.
Danielle M.P. Oliveira Fabio M. Gomes Danielle B. Carvalho Isabela Ramos Alan B. Carneiro Mario A.C. Silva-Neto Wanderley de Souza Ana P.C.A. Lima Kildare Miranda Ednildo A. Machado 《Journal of insect physiology》2013,59(12):1242-1249
Despite being the main insect pest on soybean crops in the Americas, very few studies have approached the general biology of the lepidopteran Anticarsia gemmatalis and there is a paucity of studies with embryo formation and yolk mobilization in this species. In the present work, we identified an acid phosphatase activity in the eggs of A. gemmatalis (agAP) that we further characterized by means of biochemistry and cell biology experiments. By testing several candidate substrates, this enzyme proved chiefly active with phosphotyrosine; in vitro assays suggested a link between agAP activity and dephosphorylation of egg yolk phosphotyrosine. We also detected strong activity with endogenous and exogenous short chain polyphosphates (PolyP), which are polymers of phosphate residues involved in a number of physiological processes. Both agAP activity and PolyP were shown to initially concentrate in small vesicles clearly distinct from typically larger yolk granules, suggesting subcellular compartmentalization. As PolyP has been implicated in inhibition of yolk proteases, we performed in vitro enzymatic assays with a cysteine protease to test whether it would be inhibited by PolyP. This cysteine protease is prominent in Anticarsia egg homogenates. Accordingly, short chain PolyP was a potent inhibitor of cysteine protease. We thereby suggest that PolyP hydrolysis by agAP is a triggering mechanism of yolk mobilization in A. gemmatalis. 相似文献
78.
Hoekstra M Kruijt JK Van Eck M Van Berkel TJ 《The Journal of biological chemistry》2003,278(28):25448-25453
Hepatic cholesterol(ester) uptake from serum coupled to intracellular processing and biliary excretion are important features in the removal of excess cholesterol from the body. ATP-binding cassette (ABC) transporters play an important role in hepatic cholesterol transport. The liver consists of different cell types, and ABC transporters may exert different physiological functions dependent on the individual cell type. Therefore, in the current study, using real time PCR we compared the mRNA expression of ABC transporters and genes involved in the regulation of cholesterol metabolism in liver parenchymal, endothelial, and Kupffer cells. It appears that liver parenchymal cells contain high expression levels compared with endothelial and Kupffer cells of scavenger receptor class BI ( approximately 3-fold), peroxisome proliferator-activated receptor (PPAR)alpha and PPARgamma (8-20-fold), cholesterol 7alpha-hydroxylase A1 (>100-fold), and ABCG5/G8 ( approximately 5-fold). Liver endothelial cells show a high expression of cholesterol 27-hydroxylase, liver X receptor (LXR)beta, PPARdelta, and ABCG1, suggesting a novel specific role for these genes in endothelial cells. In Kupffer cells, the expression level of LXRalpha, ABCA1, and in particular ABCG1 is high, leading to an ABCG1 mRNA expression level that is 70-fold higher than in parenchymal cells. It can be calculated that 51% of the total liver ABCG1 expression resides in Kupffer cells and 24% in endothelial cells, suggesting an intrahepatic-specific role for ABCG1 in Kupffer and endothelial cells. Because of a specific stimulation of ABCG1 in parenchymal cells by a high cholesterol diet, the contribution of parenchymal cells to the total liver increased from 25 to 60%. Our data indicate that for studies of the role of ABC transporters and their regulation in liver, their cellular localization should be taken into account, allowing proper interpretation of metabolic changes, which are directly related to their (intra)cellular expression level. 相似文献
79.
Catherine M. Phillips Audrey C. Tierney Pablo Perez‐Martinez Catherine Defoort Ellen E. Blaak Ingrid M. F. Gjelstad Jose Lopez‐Miranda Malgorzata Kiec‐Klimczak Malgorzata Malczewska‐Malec Christian A. Drevon Wendy Hall Julie A. Lovegrove Brita Karlstrom Ulf Risérus Helen M. Roche 《Obesity (Silver Spring, Md.)》2013,21(1):E154-E161
Objective:
Obesity is a key factor in the development of the metabolic syndrome (MetS), which is associated with increased cardiometabolic risk. We investigated whether obesity classification by BMI and body fat percentage (BF%) influences cardiometabolic profile and dietary responsiveness in 486 MetS subjects (LIPGENE dietary intervention study).Design and Methods:
Anthropometric measures, markers of inflammation and glucose metabolism, lipid profiles, adhesion molecules, and hemostatic factors were determined at baseline and after 12 weeks of four dietary interventions (high saturated fat (SFA), high monounsaturated fat (MUFA), and two low fat high complex carbohydrate (LFHCC) diets, one supplemented with long chain n‐3 polyunsaturated fatty acids (LC n‐3 PUFAs)).Results:
About 39 and 87% of subjects classified as normal and overweight by BMI were obese according to their BF%. Individuals classified as obese by BMI (≥30 kg/m2) and BF% (≥25% (men) and ≥35% (women)) (OO, n = 284) had larger waist and hip measurements, higher BMI and were heavier (P < 0.001) than those classified as nonobese by BMI but obese by BF% (NOO, n = 92). OO individuals displayed a more proinflammatory (higher C reactive protein (CRP) and leptin), prothrombotic (higher plasminogen activator inhibitor‐1 (PAI‐1)), proatherogenic (higher leptin/adiponectin ratio) and more insulin resistant (higher HOMA‐IR) metabolic profile relative to the NOO group (P < 0.001). Interestingly, tumor necrosis factor‐α (TNF‐α) concentrations were lower post‐intervention in NOO individuals compared with OO subjects (P < 0.001).Conclusions:
In conclusion, assessing BF% and BMI as part of a metabotype may help to identify individuals at greater cardiometabolic risk than BMI alone. 相似文献80.
Tania Fernandes Campos Aline Braga Galvão Silveira Marina Tostes Miranda Barroso 《Chronobiology international》2013,30(4):611-624
Various studies have been performed using the Social Rhythm Metric (SRM), though none has been developed with stroke patients. Stroke is a pathology that provokes a strong physical and social impact caused by an abnormality in cerebral circulation. Consequently, we performed two studies to validate the SRM and translate it into Portuguese, and to evaluate the regularity of the daily activities of stroke patients. Both healthy individuals and patients with unilateral cerebral lesions were evaluated. Subjects were of both sexes and between 45 and 65 yrs of age. Participants underwent clinical evaluation and recorded the time of 17 daily activities on the SRM for two weeks. Data were analyzed by the Pearson correlation and Fisher tests. After conceptual translation into Portuguese, corrections were made to arrive at the final version. Normative SRM scores varied from 3.2 to 7.0, suggesting that the activities presented in SRM adequately represented the daily routines of the patients. A correlation was found in SRM between the weeks (r=0.84; p=0.0001), indicating instrument reliability. The mean (±SD) score of the stoke patients was 4.8 (±1.0), and the correlation between the SRM and level of neurological damage showed that patients with lower SRM values were more physically compromised (r=?0.29; p=0.04), suggesting that SRM may be a clinical predictor. Activities related to eating and the sleep‐wake cycle were rated by most patients. In all, 71% of the patients did not work, while 84% of healthy individuals did (p=0.001). Only 64% of patients left home compared to 90% of the healthy subjects (p=0.001), and 59% of patients recorded the activity of going home compared to 82% of healthy individuals (p=0.001). According to the results, there is evidence of the validity and reliability of the SRM, enabling it to be reliably used in chronobiological studies of stroke patients. Given that a less regular lifestyle may be associated with neurological compromise and a decrease in social activities, we suggest new studies with the repeated application of this instrument over the clinical evolution of the disease to better define improvement or worsening of the patient's condition in terms of their social and health aspects. 相似文献