排序方式: 共有100条查询结果,搜索用时 17 毫秒
41.
Vrlinic T Debarnot D Legeay G Coudreuse A El Moualij B Zorzi W Perret-Liaudet A Quadrio I Mozetic M Poncin-Epaillard F 《Macromolecular bioscience》2012,12(6):830-839
New non-fouling tubes are developed and their influence on the adhesion of neuroproteins is studied. Recombinant prion proteins are considered as a single component representative of hydrophobic proteins. Samples are stored for 24?h at 4?°C in tubes coated with two different coatings: poly(N-isopropylacrylamide) as a hydrophilic surface and a plasma-fluorinated coating as a hydrophobic one. The protein adhesion is monitored by ELISA tests, XPS and confocal microscopy. It appears that the highest recovery of recombinant prion protein in the liquid phase is obtained with the hydrophilic surface while the hydrophobic character of the storage tube induces an important amount of biological loss. However, the recovery is not complete even for tubes coated with poly(N-isopropylacrylamide). 相似文献
42.
James JA Escalante CR Yoon-Robarts M Edwards TA Linden RM Aggarwal AK 《Structure (London, England : 1993)》2003,11(8):1025-1035
We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as opposed to bimodular. Altogether, the structural connection to AAA(+) proteins defines the general architecture of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for understanding the role of Rep proteins during AAV DNA replication, packaging, and site-specific integration. 相似文献
43.
Aude Lochet Régine Maury-Brachet Claire Poirier Javier Tomás Michel Lahaye Miran Aprahamian Eric Rochard 《Hydrobiologia》2008,602(1):99-109
Mercury concentration [Hg] was assessed in 20 adult Allis shad Alosa alosa (54–59 cm) and 20 adult Twaite shad Alosa fallax (36–44 cm) collected during their spawning migration in the Dordogne and the Garonne rivers (France). [Hg] was measured in
the gills, dorsal muscle, liver and kidney. Twaite shad exhibited higher [Hg] than Allis shad. Median [Hg] values were [Hg]Gills = 0.33 μg g−1 (dw), [Hg]Muscle = 1.22 μg g−1, [Hg]Liver = 1.99 μg g−1, [Hg]Kidney = 1.93 μg g−1 for Twaite shad and [Hg]Gills = 0.06 μg g−1, [Hg]Muscle = 0.20 μg g−1, [Hg]Liver = 1.18 μg g−1, [Hg]Kidney = 1.08 μg g−1 for Allis shad. In order to understand such differences, we investigated some life history traits of the two species: migratory
history, age (3–6 years), size at age (an expression of growth) and the number of spawning events (0–2 events). The difference
in estuarine residence time between the juveniles of both species, which was assumed to influence [Hg], was investigated using
otolith Sr:Ca ratio. The microchemical analysis revealed a significant difference in the residence time of juveniles in the
estuary (medians are 21 d and 10 d for Twaite shad and Allis shad, respectively) but this residence time seems too short to
influence [Hg] (Allis shad: r
spearman < 0.128; Twaite shad: r
spearman < −0.340). As both species show the same age structure, the influence of age on [Hg] was negligible. The literature shows
that the differences in growth and in the number of spawning events reported in our study are in favour of a higher [Hg] for
Twaite shad than for Allis shad. Although trophic status was not investigated here, the literature reveals that it is another
factor that could produce higher [Hg] in Twaite shad, since its diet includes higher trophic levels than Allis shad.
Guest editors: S. Dufour, E. Prévost, E. Rochard & P. Williot
Fish and diadromy in Europe (ecology, management, conservation) 相似文献
44.
The change in life history of Atlantic salmon (Salmo salar L.) on the River Dee over the last 60 years is described. Over the last 60 years, salmon have shown a change in run timing,
the majority currently entering the river between August and October compared with prior to June. This has coincided with
a change in the sea age composition, which was dominated by multi-sea winter salmon prior to the 1980s after which the proportion
of 1sea-winter fish increased until they now dominate the mature population. Growth rates of salmon in fresh water remained
relatively stable until the mid-1980s and then increased. By the end of the 1990s juvenile salmon were, by the end of their
first and second year, respectively, ∼60 and ∼19%, on average, larger than they were between the late 1930s and mid-1980s.
This has been reflected in a change in the age composition of smolts where the mean smolt age has declined from ∼2 years prior
to the 1980s to ∼1.6 years in the late 1990s. There was no observed trend in post-smolt (marine) growth for salmon. Size at
return for 1SW salmon appeared stable while there is some evidence of an increase in mean length of 2SW salmon at the end
of the 1990s. A steady state life history model was developed which suggests an increase in the instantaneous rate of mortality
by 2.9% from 1.495 year−1 in 1937/1938 to 1.538 year−1 in 1967/1969 and by 21.6% to 1.870 year−1 in 1997/1999. This is considered to explain the shift in mean age at maturity from 5.2 to 4.8 to 3.9 years for the three
periods examined. There is close agreement between the observed mean age at maturity and that predicted by the model suggesting
optimal lifetime reproductive success.
Guest editors: S. Dufour, E. Prévost, E. Rochard & P. Williot
Fish and diadromy in Europe (ecology, management, conservation) 相似文献
45.
46.
In the north Croatian Adriatic area in the period of seven years (from January 2001 to September 2007) 475 patients (39 to 80 years of age) with dry form of age related macular degeneration (AMD) were diagnosed. Complete ophthalmologic examination with special reference to visual field testing (Perimetric analysis) was performed. Peripheral visual field defects were found in 85% of patients. Elderly patients with more advanced forms of macular degeneration had more peripheral visual field defects. In 400 patients corticosteroid therapy (5 mg Prednisolonacetate, anterior H-inject, Winthorp) was administered via parabulbar injections every day/five days. Control group consisted of 75 patients treated with regular polyvitamine therapy (Lutein, Beta Karoten, Vitamin E). Patients treated with corticosteroids had peripheral visual field improvements from 10 to 25 degrees and central field improvements from 5 to 20%. In the control group treated with vitamins, central visual field showed improvements from 0.5 to 1% in 43 patients but without peripheral visual field improvements after 6 months. 相似文献
47.
The accumulation and release of polysaccharides by planktonic cells and the subsequent bacterial response during a controlled experiment 总被引:2,自引:0,他引:2
48.
The gonyaulacalean dinoflagellates Amylax spp. were recently found to contain plastids of the cryptophyte origin, more specifically of Teleaulax amphioxeia. However, not only how the dinoflagellates get the plastids of the cryptophyte origin is unknown but also their ecophysiology, including growth and feeding responses as functions of both light and prey concentration, remain unknown. Here, we report the establishment of Amylax triacantha in culture, its feeding mechanism, and its growth rate using the ciliate prey Mesodinium rubrum (= Myrionecta rubra) in light and dark, and growth and grazing responses to prey concentration and light intensity. The strain established in culture in this study was assigned to A. triacantha, based on morphological characteristics (particularly, a prominent apical horn and three antapical spines) and nuclear SSU and LSU rDNA sequences. Amylax triacantha grew well in laboratory culture when supplied with the marine mixotrophic ciliate M. rubrum as prey, reaching densities of over 7.5 × 103 cells/ml. Amylax triacantha captured its prey using a tow filament, and then ingested the whole prey by direct engulfment through the sulcus. The dinoflagellate was able to grow heterotrophically in the dark, but the growth rate was approximately two times lower than in the light. Although mixotrophic growth rates of A. triacantha increased sharply with mean prey concentrations, with maximum growth rate being 0.68/d, phototrophic growth (i.e. growth in the absence of prey) was ?0.08/d. The maximum ingestion rate was 2.54 ng C/Amylax/d (5.9 cells/Amylax/d). Growth rate also increased with increasing light intensity, but the effect was evident only when prey was supplied. Increased growth with increasing light intensity was accompanied by a corresponding increase in ingestion. In mixed cultures of two predators, A. triacantha and Dinophysis acuminata, with M. rubrum as prey, A. triacantha outgrew D. acuminata due to its approximately three times higher growth rate, suggesting that it can outcompete D. acuminata. Our results would help better understand the ecophysiology of dinoflagellates retaining foreign plastids. 相似文献
49.
Among the advantages of the single-molecule approach when used to study biomolecular structural dynamics and interaction is its ability to distinguish between and independently observe minor subpopulations. In a single-molecule Förster resonance energy transfer (FRET) and alternating laser excitation diffusion experiment, the various populations are apparent in the resultant histograms. However, because histograms are calculated based on the per-burst mean FRET and stoichiometry ratio and not on the internal photon distribution, much of the acquired information is lost, thereby reducing the capabilities of the method. Here we suggest what to our knowledge is a novel statistical analysis tool that significantly enhances these capabilities, and we use it to identify and isolate static and dynamic subpopulations. Based on a kernel density estimator and a proper photon distribution analysis, for each individual burst, we calculate scores that reflect properties of interest. Specifically, we determine the FRET efficiency and brightness ratio distributions and use them to reveal 1), the underlying structure of a two-state DNA-hairpin and a DNA hairpin that is bound to DNA origami; 2), a minor doubly labeled dsDNA subpopulation concealed in a larger singly labeled dsDNA; and 3), functioning DNA origami motors concealed within a larger subpopulation of defective motors. Altogether, these findings demonstrate the usefulness of the proposed approach. The method was developed and tested using simulations, its rationality is described, and a computer algorithm is provided. 相似文献
50.
Renza Spelat Nie Jihua Cesar Adolfo Snchez Trivio Simone Pifferi Diletta Pozzi Matteo Manzati Simone Mortal Irene Schiavo Federica Spada Melania Eva Zanchetta Tamara Ius Ivana Manini Irene Giulia Rolle Pietro Parisse Ana P. Milln Ginestra Bianconi Fabrizia Cesca Michele Giugliano Anna Menini Daniela Cesselli Miran Skrap Vincent Torre 《Cell death & disease》2022,13(8)
Seizures represent a frequent symptom in gliomas and significantly impact patient morbidity and quality of life. Although the pathogenesis of tumor-related seizures is not fully understood, accumulating evidence indicates a key role of the peritumoral microenvironment. Brain cancer cells interact with neurons by forming synapses with them and by releasing exosomes, cytokines, and other small molecules. Strong interactions among neurons often lead to the synchronization of their activity. In this paper, we used an in vitro model to investigate the role of exosomes released by glioma cell lines and by patient-derived glioma stem cells (GSCs). The addition of exosomes released by U87 glioma cells to neuronal cultures at day in vitro (DIV) 4, when neurons are not yet synchronous, induces synchronization. At DIV 7–12 neurons become highly synchronous, and the addition of the same exosomes disrupts synchrony. By combining Ca2+ imaging, electrical recordings from single neurons with patch-clamp electrodes, substrate-integrated microelectrode arrays, and immunohistochemistry, we show that synchronization and de-synchronization are caused by the combined effect of (i) the formation of new neuronal branches, associated with a higher expression of Arp3, (ii) the modification of synaptic efficiency, and (iii) a direct action of exosomes on the electrical properties of neurons, more evident at DIV 7–12 when the threshold for spike initiation is significantly reduced. At DIV 7–12 exosomes also selectively boost glutamatergic signaling by increasing the number of excitatory synapses. Remarkably, de-synchronization was also observed with exosomes released by glioma-associated stem cells (GASCs) from patients with low-grade glioma but not from patients with high-grade glioma, where a more variable outcome was observed. These results show that exosomes released from glioma modify the electrical properties of neuronal networks and that de-synchronization caused by exosomes from low-grade glioma can contribute to the neurological pathologies of patients with brain cancers.Subject terms: Neuroscience, Preclinical research 相似文献