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排序方式: 共有720条查询结果,搜索用时 15 毫秒
711.
Mira Starović Slobodan Kuzmanović Veljko Gavrilović Goran Aleksić Tatjana Popović Saša Stojanović Dragana Jošić 《Journal of Phytopathology》2012,160(11-12):758-760
Plants of alfalfa (Medicago sativa) exhibiting general stunting, proliferation and phyllody associated with leaf yellowing and reddening were observed in three localities of Central Serbia. Phytoplasma strains belonging to 16SrIII‐B and 16SrXII‐A groups were detected and identified by RFLP and sequence analysis of 16S rDNA. Stolbur phytoplasma tuf gene RFLP analysis showed the presence of the TufAY‐b‐type phytoplasma subgroup in 80% of symptomatic samples. This is the first report of 16SrIII‐B and 16SrXII‐A phytoplasma groups affecting alfalfa in Serbia. 相似文献
712.
Histatins are salivary cationic peptides that provide the first line of defense against oral candidiasis caused by Candida albicans. This minireview presents a critical evaluation of our knowledge of the candidacidal mechanism of histatin 5 (Hst 5). Hst 5 is the most potent among all histatin family members with regard to its antifungal activity. The mode of action of Hst 5 has been a subject of intense debate. Unlike other classical host innate immune proteins, pore formation or membrane lysis by Hst 5 has largely been disproven, and it is now known that all targets of Hst 5 are intracellular. Hst 5 binds C. albicans cell wall proteins (Ssa1/2) and glycans and is taken up by the cells through fungal polyamine transporters in an energy-dependent manner. Once inside the fungal cells, Hst 5 may affect mitochondrial functions and cause oxidative stress; however, the ultimate cause of cell death is by volume dysregulation and ion imbalance triggered by osmotic stress. Besides these diverse targets, a novel mechanism based on the metal binding abilities of Hst 5 is discussed. Finally, translational approaches for Hst 5, based on peptide design and synergy with other known drugs, are considered a step forward for bench-to-bed application of Hst 5. 相似文献
713.
SDS-polyacrylamide gel electrophoresis of membrane proteins: effect of phospholipids 总被引:1,自引:0,他引:1
Under the conditions described in this report, it was found that the occurrence of phospholipids in membrane samples has no artifactual impact on the subsequent separation and visualization of membrane proteins in SDS-polyacrylamide gels stained with Coomassie Blue. 相似文献
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716.
Anton A. Reznicek María del Socorro González-Elizondo Marlene Hahn Mira Garner Andrew L. Hipp 《植物分类学报:英文版》2021,59(4):698-725
Carex section Schiedeanae (subg. Euthyceras) has long been thought to comprise at most five species of Mexican sedges. Our morphological studies in the field and herbarium, however, supported by more recent DNA sequencing work, demonstrate that the section is, in fact, three times as species-rich, making it one of the largest sections in Mexico. Its 15 species range from Chihuahua to Tamaulipas south to Veracruz and Oaxaca; a single species, Carex muriculata, extends into the United States. The section has unusual inflorescences with simple (except in Carex complexa), uniformly androgynous, sessile spikes, utriculiform cladoprophylls, and perigynia with a unique combination of pubescence types composed of papillae; unicellular, simple hairs; and (in many species) multicellular tubercles. We recognize five previously described species (C. muriculata,Carex perstricta,Carex schiedeana, Carex stellata, and Carex vizarronensis) and describe 10 additional species (Carex angustilepis, Carex cabralii, Carex complexa, Carex dentata, Carex gypsophila, Carex mesophila, Carex paneroi, Carex planilamina, Carex revoluta, and Carex tehuacana). We have expanded the morphological features used in the section to include particularly anther, staminate scale, and style features, and more detailed micromorphology of the perigynia, especially indumentum. In total, 13 of the 15 species recognized herein, with a total of 32 individuals, were examined for ETS and ITS for phylogenetic reconstruction. Phylogenetically, the xeric species (plus C. paneroi) form a grade leading to a clade of the more mesophytic species adapted to forest understories, plus the unique cliff dwelling C. complexa. 相似文献
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Ji‐Eun Lee Da‐Eun Jo An‐Jung Lee Hye‐Kyung Park Kumju Youn Eun‐Young Yun Jae‐Sam Hwang Mira Jun Byoung Heon Kang 《Entomological Research》2014,44(6):244-253
Protaetia brevitarsis larvae have been used as a traditional medicine to treat various liver diseases. Freeze‐dried powder and solvent partitioned fractions of Protaetia brevitarsis larvae, in this study, were examined for hepatoprotective and antitumor activities in vivo and in vitro. Oral administration of the powder reduced signs of acute and chronic liver injuries in diethylnitrosamine‐induced hepatotoxic mouse model. Various cancer cell types, but not normal primary hepatocytes, were killed by the hexane, ethyl acetate and butanol fractions, which were partitioned from larval extracts. Apoptosis and autophagy were primarily induced in cancer cells by hexane and ethyl acetate fractions, respectively, and can be the cytotoxic mechanisms of the fractions. Further analyses of the insect fractions could lead to identification of novel bioactive substances that can be exploited as therapeutics for various human liver diseases. 相似文献
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720.
M. Mira G. Alperstein M. Karr G. Ranmuthugala J. Causer A. Niec A. M. Lilburne 《BMJ (Clinical research ed.)》1996,312(7035):881-883
OBJECTIVE: To compare the intakes of haem and non-haem iron in iron depleted and iron replete children. DESIGN: Case-control study. SETTING: Early Childhood Centres and a long day care centre in Sydney, Australia. SUBJECTS: Children aged 12-36 months depleted in iron and controls matched for age and sex. MEAN OUTCOME MEASURES: Iron status by using plasma ferritin concentration. A three day weighed dietary intake record completed by the parents. Risk factors for iron deficiency assessed by questionnaire. RESULTS: Fifty six iron depleted and 68 iron replete children participated. The average daily intake of haem iron was significantly lower in the iron depleted group (t = 2.392, P = 0.018); there was a tendency towards a lower average daily intake of non-haem iron (t = 1.724, P = 0.086) and vitamin C (t = 1.921, P = 0.057) for iron depleted children. Low intake of haem iron (< 0.71 mg/day) was significantly associated with iron depletion with an odds ratio fo 3.0 (P = 0.005). The proportion of iron depleted children who were given whole cows'' milk before 12 months of age was almost double that of iron replete children; multivariate analysis showed that both haem iron intake and age of introduction of cows'' milk were independently associated with iron depletion. CONCLUSIONS: The results of this study show that, in young children in developed countries, a lower haem iron intake is a major risk factor for iron depletion; the introduction of whole cows'' milk before 12 months is further confirmed as a risk factor. Parental education on nutrition should now focus on these two aspects of nutrition for infants and young children. 相似文献