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641.
Ginzberg I Perl A Genser M Wininger S Nemas C Kapulnik Y 《Journal of plant physiology》2004,161(5):611-620
Biotin is an essential cofactor for a variety of carboxylase and decarboxylase reactions and is involved in diverse metabolic pathways of all organisms. In the present study we tested the hypothesis that controlling biotin availability by the expression of Streptomyces avidinii streptavidin, would impede plant development. Transient expression of streptavidin fused to plant signal peptide, bacterial signal peptide or both, in tomato (Lycopersicon esculentum cv. VF36) plants resulted in various levels of tissue impairment, exhibited as lesion development on 1-week-old tomato seedlings. The least toxic construct was introduced to tomato (stable transformation) under the constitutive CaMV 35S promoter, and lesions appeared on stems, flower morphologies were modified and numbers and sizes of fruits were altered. Furthermore, tissue-specific expression of the streptavidin, by means of the beta-phaseolin or TobRB7 promoters, resulted in localised effects, i.e., impaired seed formation or seedless fruits, respectively, with no alteration in the morphology of the other plant organs. External application of biotin on streptavidin-expressing tomato plants prevented the degeneration symptoms and facilitated normal plant development. It can be concluded that expression of streptavidin in the plant cell can lead to local and temporal deficiencies in biotin availability, impairing developmental processes while biotin application restores plant growth cycle. 相似文献
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644.
Helena?Mira Mar?al?Vilar Vicent?Esteve Marc?Martinell Marcelo?J?Kogan Ernest?Giralt David?Salom Ismael?Mingarro Lola?Pe?arrubia Enrique?Pérez-PayáEmail author 《BMC structural biology》2004,4(1):7
Background
Arabidopsis thaliana copper metallochaperone CCH is a functional homologue of yeast antioxidant ATX1, involved in cytosolic copper transport. In higher plants, CCH has to be transported to specialised cells through plasmodesmata, being the only metallochaperone reported to date that leaves the cell where it is synthesised. CCH has two different domains, the N-terminal domain conserved among other copper-metallochaperones and a C-terminal domain absent in all the identified non-plant metallochaperones. The aim of the present study was the biochemical and biophysical characterisation of the C-terminal domain of the copper metallochaperone CCH. 相似文献645.
Evidence for the plant-specific intercellular transport of the Arabidopsis copper chaperone CCH 总被引:11,自引:0,他引:11
Mira H Martínez-García F Peñarrubia L 《The Plant journal : for cell and molecular biology》2001,25(5):521-528
Arabidopsis copper chaperone (CCH) belongs to a family of eukaryotic proteins that participates in intracellular copper homeostasis by delivering this metal to the secretory pathway. In this work we show that the CCH protein is mainly located along the vascular bundles of senescing leaves and petioles, as shown by tissue prints and immunohistochemical detection. CCH protein also accumulates in stem sieve elements and is collected in phloem exudates. Accordingly, Arabidopsis CCH is the only member of the metallochaperone family described to function intercellularly to date. Moreover, the CCH protein remains stable when plants are subjected to excess copper that causes a rapid and specific decrease in its mRNA. These facts point to a role for CCH in copper mobilization from decaying organs towards reproductive structures, as a result of metalloprotein breakdown. 相似文献
646.
García-Martos P Marín P Hernández-Molina JM García-Agudo L Aoufi S Mira J 《Mycopathologia》2001,150(1):1-4
The extracellular enzymatic activity of 36 strains of yeast belonging to 11 species of the genus Cryptococcus, has been investigated, using the API-ZYM (BioMérieux, France) commercial system, with the objective of determining the differences
in the enzymatic profiles of the various species. The strains studied were : 9 of C. neoformans, 7 of C. albidus, 6 of C. laurentii, 5 of C. uniguttulatus, 3 of C. humicolus, and 1 each of C. ater, C. curvatus, C. dimennae, C. hungaricus, C. infirmo-miniatus and C. magnus. All the strains showed enzymatic activity with positivity to Phosphatase alkaline, Esterase lipase C8, Leucine arylamidase,
Phosphatase acid and Naphthol-AS-BI-phosphohydrolase, and negativity to Lipase C14, Trypsin, Chemotrypsin, β-galactosidase,
β-glucuronidase and α-manosidase. Variable enzymatic activity was shown to Esterase C4, Valine arylamidase, Cystine arylamidase,
α-galactosidase,α-glucosidase, β-glucosidase, N-acetyl-β-glucosaminidase and α-fucosidase. This allowed 11 separate enzymatic
patterns to be established. The species C. neoformans and C. laurentii each presented two distinct patterns; C. uniguttulatus, C. hungaricus andC. magnus shared the same pattern; C. albidus, C. ater, C. curvatus,C. dimennae, C. humicolus and C. infirmo-miniatus presented an individual enzymatic pattern. The results obtained suggest that the API-ZYM system could be useful for the identification
of species of the genus Cryptococcus and for the differentiation of the enzymotypes for epidemiological purposes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
647.
Mira A 《Journal of insect physiology》2000,46(4):605-610
Many insects eat their cast cuticle (exuviae) after moulting. The functional significance of this behaviour has not been addressed experimentally. I tested the hypothesis that exuviae eating constitutes a meal, so the animal recycles its nitrogen content. Nitrogenous compounds (protein and chitin) are major components of the cuticle in Periplaneta americana, accounting for as much as 87% of the total weight. It was found that insects almost invariably ate their exuviae during their larval life. The frequency of the behaviour decreased in newly emerged adults and varied between the sexes, males eating their exuviae less frequently than females. This may be due to the extra nitrogen endowment which females need for reproduction. Aposymbiotic animals, which lack the supply of essential amino acids from endosymbiotic bacteria, always ate their exuviae regardless of sex. When animals were reared on different diets throughout their larval life protein level in the diet correlated with exuviae eating. Animals reared on a low protein diet showed the highest levels of exuviae eating; animals reared on a high protein diet showed the highest levels of exuviae rejection. Analysis of the frass produced after exuviae meals showed that over 58% of the nitrogen present in the exuviae was recycled. This demonstrated that cockroaches digested nitrogenous compounds contained in the cuticle. The possibility that the exuviae meal has other functions is discussed, although the evidence supports a nutritional role. 相似文献
648.
We compared nutrient selection in a laboratory culture of the American cockroach (Periplaneta americana: Insecta, Blattodea) and a feral population which was founded by escapees from the culture approximately 14 generations prior to the study. In a first experiment, adult male and female cockroaches were provided with two nutritionally complementary synthetic foods, and thus allowed to select the protein and carbohydrate components of their diet independently. There were no differences in the amounts of carbohydrate eaten by the two populations. However, feral males ingested more protein than cultured males. In a second experiment, the construction of nitrogen budgets showed that the additional nitrogen ingested by feral males was allocated preferentially to accessory sex glands, rather than somatic tissue or excretion via the faeces. This suggests a possible role for sexual selection in the dietary difference between the strains. By contrast with males, there was no statistically significant difference in the amount of protein eaten by females of the two populations. However, feral females were found to have a higher density of bacterial endosymbionts than cultured females. Since these symbionts are involved in the synthesis of essential amino acids, this might account for greater reproductive output observed in a previous study in the feral compared with the culture females. 相似文献
649.
Throughout evolution, both prokaryotic and eukaryotic cells have developed a variety of biochemical mechanisms to define the direction and proximity of extracellular stimuli. This process is essential for the cell to reply properly to the environmental cues that determine cell migration, proliferation, and differentiation. Chemotaxis is the cellular response to chemical attractants that direct cell migration, a process that plays a central role in many physiological situations, such as host immune responses, angiogenesis, wound healing, embryogenesis, and neuronal patterning, among others. In addition, cell migration takes part in pathological states, including inflammation and tumor metastasis. Indeed, tumor progression to invasion and metastasis depends on the active motility of the invading cancer cells and the endothelial cell bed during tumor neovascularization. Cell migration switches "off" and "on," based on quantitative differences in molecular components such as adhesion receptors, cytoskeletal linking proteins, and extracellular matrix ligands, and by regulating the affinity of membrane-bound chemoattractant receptors. A clear understanding of how cells sense chemoattractants is, therefore, of pivotal importance in the biology of the normal cell as well as in prevention of malignant cell invasion. Here we offer a perspective on cell migration that emphasizes the relationship between cell polarization and cell movement and the importance of the equilibrium between the signals that drive each process for the control of tumor cell invasion. 相似文献
650.
Zygosaccharomyces bailii is the most tolerant yeast species to acetic acid-induced toxicity, being able to grow in the presence of concentrations of this food preservative close to the legal limits. For this reason, Z. bailii is the most important microbial contaminant of acidic food products but the mechanisms behind this intrinsic resistance to acetic acid are very poorly characterized. To gain insights into the adaptive response and tolerance to acetic acid in Z. bailii, we explored an expression proteomics approach, based on quantitative 2DE, to identify alterations occurring in the protein content in response to sudden exposure or balanced growth in the presence of an inhibitory but nonlethal concentration of this weak acid. A coordinate increase in the content of proteins involved in cellular metabolism, in particular, in carbohydrate metabolism (Mdh1p, Aco1p, Cit1p, Idh2p, and Lpd1p) and energy generation (Atp1p and Atp2p), as well as in general and oxidative stress response (Sod2p, Dak2p, Omp2p) was registered. Results reinforce the concept that glucose and acetic acid are coconsumed in Z. bailii, with acetate being channeled into the tricarboxylic acid cycle. When acetic acid is the sole carbon source, results suggest the activation of gluconeogenic and pentose phosphate pathways, based on the increased content of several proteins of these pathways after glucose exhaustion. 相似文献