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131.
Hans G. Drexler Mira Menon Gerhard Gaedicke Jun Minowada 《Cancer immunology, immunotherapy : CII》1986,21(3):188-192
Summary The reactivity with monoclonal antibodies (MoAbs) specific for myelomonocytic cells and the expression of a particular esterase isoenzyme were analyzed in 159 cases of acute myeloid leukemias. The incidence of positivity of 16 MoAbs (MCS-2, MCS-1, OKM1, My-1, Leu-M1, Leu-M3, CA-2-38, MY4, MY7, MY8, MY9, VIM-D2, VIM-D5, Mo1, Mo2, 63D3) was studied using the indirect immunofluorescence technique. A carboxylic esterase isoenzyme which can be inhibited completely and selectively by sodium fluoride (NaF) was demonstrated by isoelectric focusing on horizontal polyacrylamide gels. This NaF-sensitive isoenzyme indicated the monocytic origin of the blast cells as it is specific for this cell lineage. Prior to the immunological-isoenzymatic analysis all cases were categorized into two subtypes according to morphological criteria of the FAB classification system: 147 cases of AML (FAB M1-3) and 12 cases of AMMoL/AMoL (FAB M4/5). However, 15 out of 147 cases of AML expressed the NaF-sensitive isoenzyme and were therefore assigned to the group AMMoL/AMoL. Likewise, 1 case, diagnosed morphologically as AMMoL, was negative for this marker isoenzyme and was assigned to the other leukemia subtype. The incidence of reactivity varied widely for the MoAbs tested regarding the overall results on all cases and the positivity on cases of either AML or AMMoL/AMoL. The MoAbs were grouped into four classes depending on the pattern of reactivity with myeloblastic or monoblastic or both subtypes of acute myeloid leukemia. The MoAbs MCS-2, MY7, Leu-M1, and MY9 detected the vast majority of cases with either myelocytic or monocytic involvement (group-I: pan-myelomonocytic reactivity). The MoAbs MCS-1, OKM1, VIM-D5, and Mo1 showed a predominance in their staining pattern for monocytic variants, but were also positive on a substantial percentage of nonmonocytic cases (group-II: predominantly reactive with monocytic, but also myelocytic cases). The MoAbs Leu-M3, MY4, VIM-D2, Mo2, and MY8 reacted with the large majority of AMMoL/AMoL cases and with a small number of AML cases (group-III: monocyte-specific reactivity). The MoAbs of group-I are useful in differentiating acute lymphoid from acute myeloid leukemias. The MoAbs of group-III, and to a lower extent those of group-II, will be of considerable value in the subtyping of acute myeloid leukemias. The results show that (1) accuracy of leukemia classification might not always be achieved by morphology alone, but that immunological and biochemical aspects should be included as well, and (2) several MoAbs are very useful tools for classification and subtyping of acute myeloid leukemias. 相似文献
132.
133.
Summary A study has been made of the mineral requirements of Streptomyces kanamyceticus KCC S-0433 for production of glucose isomerase. The optimal concentrations of MgSO4 and K2HPO4 for enzyme production are 0.07% and 0.05%, respectively. The elements Fe, Mn and Zn are required at levels of 10, 3 and 3 mg/l, respectively. Cu, Co and Ca have inhibitory effects on the production of the enzyme. 相似文献
134.
Titmice are a better indicator of bird density in Northern European than in Western European forests
Mira H. Kajanus Jukka T. Forsman Maximilian G. R. Vollstdt Vincent Devictor Merja Elo Aleksi Lehikoinen Mikko Mnkknen James T. Thorson Sami M. Kivel 《Ecology and evolution》2022,12(2)
Population sizes of many birds are declining alarmingly and methods for estimating fluctuations in species’ abundances at a large spatial scale are needed. The possibility to derive indicators from the tendency of specific species to co‐occur with others has been overlooked. Here, we tested whether the abundance of resident titmice can act as a general ecological indicator of forest bird density in European forests. Titmice species are easily identifiable and have a wide distribution, which makes them potentially useful ecological indicators. Migratory birds often use information on the density of resident birds, such as titmice, as a cue for habitat selection. Thus, the density of residents may potentially affect community dynamics. We examined spatio‐temporal variation in titmouse abundance and total bird abundance, each measured as biomass, by using long‐term citizen science data on breeding forest birds in Finland and France. We analyzed the variation in observed forest bird density (excluding titmice) in relation to titmouse abundance. In Finland, forest bird density linearly increased with titmouse abundance. In France, forest bird density nonlinearly increased with titmouse abundance, the association weakening toward high titmouse abundance. We then analyzed whether the abundance (measured as biomass) of random species sets could predict forest bird density better than titmouse abundance. Random species sets outperformed titmice as an indicator of forest bird density only in 4.4% and 24.2% of the random draws, in Finland and France, respectively. Overall, the results suggest that titmice could act as an indicator of bird density in Northern European forest bird communities, encouraging the use of titmice observations by even less‐experienced observers in citizen science monitoring of general forest bird density. 相似文献
135.
Natalia Molinero Diego Taladrid Irene Zorraquín-Pea Miguel de Celis Ignacio Belda Alex Mira Begoa Bartolom M. Victoria Moreno-Arribas 《Current issues in molecular biology》2022,44(4):1513
Ulcerative colitis (UC) is a recurrent pathology of complex etiology that has been occasionally associated with oral lesions, but the overall composition of the oral microbiome in UC patients and its role in the pathogenesis of the disease are still poorly understood. In this study, the oral microbiome of UC patients and healthy individuals was compared to ascertain the possible changes in the oral microbial communities associated with UC. For this, the salivary microbiota of 10 patients diagnosed with an active phase of UC and 11 healthy controls was analyzed by 16S rRNA gene sequencing (trial ref. ISRCTN39987). Metataxonomic analysis revealed a decrease in the alpha diversity and an imbalance in the relative proportions of some key members of the oral core microbiome in UC patients. Additionally, Staphylococcus members and four differential species or phylotypes were only present in UC patients, not being detected in healthy subjects. This study provides a global snapshot of the existence of oral dysbiosis associated with UC, and the possible presence of potential oral biomarkers. 相似文献
136.
A major target for environmental managers when trying to minimise the road-barrier effect on wildlife is to improve permeability
to animal movements. Previous studies have demonstrated that drainage culverts are used by vertebrates, although knowledge
of the main influencing factors remains limited. The use of 34 culverts from two roads in southern Portugal, differing in
traffic volume, vehicle speeds and configuration, was evaluated by the analysis of terrestrial vertebrate footprint data (408
passage-operative days). Culvert crossings were related to various explanatory variables by means of canonical ordination
techniques. We recorded 901 complete crossings, corresponding to an average of 2.2 crossings/culvert/operative day. Thirteen
taxa were detected, all in more than one passage. Animal species included reptiles, small mammals, lagomorphs, carnivores
and domestic dogs and cats. Our results suggest that fencing might have a funnelling effect, directing larger animals toward
culverts. Also, vegetation covering culvert entrances seems to have a positive effect, particularly on genets; longer passages
with entrances far from the pavement were, apparently, avoided by smaller animals; a lower number of crossings was detected
on passages with detritus pits; the closest passages to urban areas are more often used by domestic species; forest-living
species favour passages with low, open land cover nearby; and smaller species, like lagomorphs and small mammals, appear to
use more culverts near the pavement, which probably reflects the importance of road verges as refuges for these species. Although
not used by all species present in the study area, constructing numerous passages of different sizes without detritus pits
and which are distributed along roads might be an important step in mitigating road fragmentation effects on animal populations. 相似文献
137.
AIMS: To study arginine degradation and carcinogenic ethyl carbamate precursor citrulline formation during and after malolactic fermentation (MLF). METHODS AND RESULTS: MLF was induced in white wine with two commercial Oenococcus oeni strains under different winemaking conditions regarding the type of alcoholic fermentation (spontaneous, induced) and the lees management (racked, on lees). Arginine degradation and citrulline formation did not occur during malic acid degradation in any treatment. In five of the six treatments in which arginine degradation took place, it occurred 3 weeks after malic acid depletion and significant amounts of citrulline were formed. Presence of yeast lees in wines led to increased citrulline formation. Conclusions: This study suggests that arginine metabolism is inhibited in oenococci at low pH values (< 3.5) and that in the postalcoholic fermentation phase, citrulline formation from arginine degradation can be avoided if MLF is induced by pure cultures of O. oeni with inhibition of the bacterial biomass after malic acid depletion. Residual yeast lees in the wine have been identified as a significant risk factor for increased citrulline formation. SIGNIFICANCE AND IMPACT OF THE STUDY: Conclusions drawn from this study allow reducing the risk of carcinogenic ethyl carbamate formation from citrulline excretion by wine lactic acid bacteria. 相似文献
138.
Terezija Hr?enjak Maja Popovi? Tamara Bo?i? Mira Grdisa Dorde Kobrehel Ljerka Tiška-Rudman 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1998,119(4):825-832
Biologically active glycolipoprotein complex (G-90) isolated from whole earthworm tissue extract shows anticoagulative and fibrinolytic activities. We isolated two tyrosine like serine peptidases with molecular masses of 34 kDa (P I) and 23 kDa (P II), respectively. P I peptidase is autocatalytically degraded to P II. Both peptidases exhibit fibrinolytic and anticoagulative activities. The activity of P I is much higher. P I in concentration of 105 ng ml−1 of plasma shortened the physiological time of fibrin clot lysis by 54% and completely inhibited blood clotting at a concentration of 103 ng ml−1 of venous blood. 相似文献
139.
Lee JC Weissglas-Volkov D Kyttälä M Dastani Z Cantor RM Sobel EM Plaisier CL Engert JC van Greevenbroek MM Kane JP Malloy MJ Pullinger CR Huertas-Vazquez A Aguilar-Salinas CA Tusie-Luna T de Bruin TW Aouizerat BE van der Kallen CC Croce CM Aqeilan RI Marcil M Viikari JS Lehtimäki T Raitakari OT Kuusisto J Laakso M Taskinen MR Genest J Pajukanta P 《American journal of human genetics》2008,83(2):180-192
Low serum HDL-cholesterol (HDL-C) is a major risk factor for coronary artery disease. We performed targeted genotyping of a 12.4 Mb linked region on 16q to test for association with low HDL-C by using a regional-tag SNP strategy. We identified one SNP, rs2548861, in the WW-domain-containing oxidoreductase (WWOX) gene with region-wide significance for low HDL-C in dyslipidemic families of Mexican and European descent and in low-HDL-C cases and controls of European descent (p = 6.9 × 10−7). We extended our investigation to the population level by using two independent unascertained population-based Finnish cohorts, the cross-sectional METSIM cohort of 4,463 males and the prospective Young Finns cohort of 2,265 subjects. The combined analysis provided p = 4 × 10−4 to 2 × 10−5. Importantly, in the prospective cohort, we observed a significant longitudinal association of rs2548861 with HDL-C levels obtained at four different time points over 21 years (p = 0.003), and the T risk allele explained 1.5% of the variance in HDL-C levels. The rs2548861 resides in a highly conserved region in intron 8 of WWOX. Results from our in vitro reporter assay and electrophoretic mobility-shift assay demonstrate that this region functions as a cis-regulatory element whose associated rs2548861 SNP has a specific allelic effect and that the region forms an allele-specific DNA-nuclear-factor complex. In conclusion, analyses of 9,798 subjects show significant association between HDL-C and a WWOX variant with an allele-specific cis-regulatory function. 相似文献
140.
Čedomila Milin Robert Domitrović Marin Tota Jasminka Giacometti Mira Ćuk Biserka Radošević-Stašić Zlatko Ciganj 《Biological trace element research》2001,82(1-3):201-210
The mineral content (zinc, iron, magnesium, and calcium) in the liver, spleen, and thymus of male Balb/C mice was analyzed.
Animals were fed, over 21 d, diets enriched with corn oil (FCO diet) or olive oil (FOO diet) (5% addition to standard pellet,
w/w). Olive oil with predominant oleic acid (C18:1, n-9) had a quite different composition than corn oil, in which linoleic
acid (C18:2, n-6) prevails. The zinc and magnesium tissue concentrations were not changed in either group. The calcium concentration
in liver as well as the calcium concentration in spleen increased in mice fed both the FCO and FOO diets. Furthermore, mice
fed both the FOO and FCO diets had increased spleen iron concentration. Mice fed the FCO diet had increased thymus calcium
concentration compared to controls. The results show the effect of diets with unsaturated, particularly polyunsaturated fatty
acids, on the calcium and iron concentration in some organs. 相似文献