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81.
Use of the green fluorescent protein as an educational tool 总被引:1,自引:0,他引:1
The green fluorescent protein (GFP) is a bioluminescent protein that can be expressed and easily detected as a fully fluorescent
protein in both bacterial and eukaryotic cells. These properties, along with its ability to withstand exposure to denaturants,
organic solvents, high temperature and a wide pH range, make GFP an ideal educational tool. To that end, two GFP-based laboratory
modules are described that can be used to teach recombinant DNA and protein purification techniques to high school and undergraduate
college students. Journal of Industrial Microbiology & Biotechnology (2000) 24, 323–326.
Received 02 April 1999/ Accepted in revised form 20 November 1999 相似文献
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84.
Wang Liang Tang Dalin Maehara Akiko Molony David Zheng Jie Samady Habib Wu Zheyang Lu Wenbin Zhu Jian Ma Genshan Giddens Don P. Stone Gregg W. Mintz Gary S. 《Biomechanics and modeling in mechanobiology》2019,18(5):1269-1280
Biomechanics and Modeling in Mechanobiology - Plaque progression and vulnerability are influenced by many risk factors. Our goal is to find a simple method to combine multiple risk factors for... 相似文献
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A Amsterdam R Riesel Y Mintz M Shemesh Y Salomon 《Biochemical and biophysical research communications》1979,87(2):505-512
Follicular fluid obtained from medium or large bovine ovarian follicles inhibited ovarian luteinizing hormone/human chorionic gonadotropin sensitive adenylate cyclase in a dose-dependent manner (I50 = 3 mg follicular fluid protein/ml). The inhibitory activity was excluded by Sephadex G-10 and was fully retained following treatment with charcoal. Fluoride-stimulated enzyme activity was not inhibited. Binding of 125I human chorionic gonadotropin to ovarian plasma membranes was only slightly reduced by the follicular fluid. The post-microsomal supernatant of homogenates from ovaries of immature (27-day-old) rats collected 24–36 h after treatment with 15 i.u. of pregnant mare serum gonadotropin also inhibited luteinizing hormone-sensitive adenylate cyclase. The extent of this inhibition seemed to decline with follicular maturation. The possibility is raised that ovarian sulfated glycosaminoglycans are responsible for the observed inhibition of adenylate cyclase. 相似文献
87.
Heparin was found to be the most potent inhibitor of rat ovarian luteinizing hormone-sensitive adenylate cyclase (I50 = 2 microgram/ml) when compared to other naturally occurring glycosamin oglycans. This inhibition was also apparent when this enzyme was stimulated by follicle-stimulating hormone or prostaglandin E2. Heparin was also found to inhibit glucagon-sensitive rat hepatic adenylate cyclase, and the prostaglandin E1-sensitive enzyme from rat ileum and human platelets. In contrast, heparin stimulated the dopamine sensitive adenylate cyclase from rat caudate nucleus. The sulfated polysugar dextran sulfate exerts similar effects on adenylate cyclase activity of the rat ovary and was shown to inhibit hormone binding to rat ovarian plasma membrane in a manner similar to that exerted by heparin. In contrast to heparin, dextran sulfate inhibited dopamine-sensitive adenylate cyclase from rat caudate nucleus. 相似文献
88.
Tissue analyses of mouse β-galactosidase (BGS) activity indicate previously unobserved genetic complexities. The existing genotypic classification of Bgsh (high-activity) vs Bgsd (diminished-activity) alleles has been based exclusively on strain differences in brain [Felton, J., Meisler, M., and Paigen, K. (1974). J. Biol. Chem.249, 3267–3272]. However, it now appears that other tissues in a strain with “high BGS” in brain can be relatively low in activity, or that tissues in a “low-BGS” strain can be relatively high. In addition, different cell types within a tissue (e.g., exocrine vs endocrine pancreas) can vary in BGS activity independently of each other. A given tissue ranges, among strains tested here, between 1.8- and 3.6-fold over the activity of the lowest strain. These new results suggest that complex mechanisms may be involved in tissue- and cell type-specific control of BGS activity. The hypothesis is proposed that there may be “controlling genes” determining specific enzyme levels by causing a limited somatic amplification of the structural gene and by controlling the degree of amplification in a given cell type and strain. 相似文献
89.
Mikhail Menis Richard A. Forshee Sanjai Kumar Stephen McKean Rob Warnock Hector S. Izurieta Rahul Gondalia Chris Johnson Paul D. Mintz Mark O. Walderhaug Christopher M. Worrall Jeffrey A. Kelman Steven A. Anderson 《PloS one》2015,10(10)
Background
Human babesiosis, caused by intraerythrocytic protozoan parasites, can be an asymptomatic or mild-to-severe disease that may be fatal. The study objective was to assess babesiosis occurrence among the U.S. elderly Medicare beneficiaries, ages 65 and older, during 2006–2013.Methods
Our retrospective claims-based study utilized large Medicare administrative databases. Babesiosis occurrence was ascertained by recorded ICD-9-CM diagnosis code. The study assessed babesiosis occurrence rates (per 100,000 elderly Medicare beneficiaries) overall and by year, age, gender, race, state of residence, and diagnosis months.Results
A total of 10,305 elderly Medicare beneficiaries had a recorded babesiosis diagnosis during the eight-year study period, for an overall rate of about 5 per 100,000 persons. Study results showed a significant increase in babesiosis occurrence over time (p<0.05), with the largest number of cases recorded in 2013 (N = 1,848) and the highest rates (per 100,000) in five Northeastern states: Connecticut (46), Massachusetts (45), Rhode Island (42), New York (27), and New Jersey (14). About 75% of all cases were diagnosed from May through October. Babesiosis occurrence was significantly higher among males vs. females and whites vs. non-whites.Conclusion
Our study reveals increasing babesiosis occurrence among the U.S. elderly during 2006–2013, with highest rates in the babesiosis-endemic states. The study also shows variation in babesiosis occurrence by age, gender, race, state of residence, and diagnosis months. Overall, our study highlights the importance of large administrative databases in assessing the occurrence of emerging infections in the United States. 相似文献90.
Duijnisveld BJ Bigot A Beenakker KG Portilho DM Raz V van der Heide HJ Visser CP Chaouch S Mamchaoui K Westendorp RG Mouly V Butler-Browne GS Nelissen RG Maier AB 《Arthritis research & therapy》2011,13(6):R207-10