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Oumaïma Ibrahim-Granet Grégory Jouvion Tobias M Hohl Sabrina Droin-Bergère François Philippart Oh Yoen Kim Minou Adib-Conquy Reto Schwendener Jean-Marc Cavaillon Matthias Brock 《BMC microbiology》2010,10(1):105
Background
Invasive aspergillosis (IA) is a major cause of infectious morbidity and mortality in immune compromised patients. Studies on the pathogenesis of IA have been limited by the difficulty to monitor disease progression in real-time. For real-time monitoring of the infection, we recently engineered a bioluminescent A. fumigatus strain. 相似文献63.
Relaxation measurements on the kinetics of the double helix to coil transition for the self-complementary ribo-oligonucleotide A7U7 are reported over a concentration range of 6.9 μM to 19.6 μM in single strand in 1 M NaCl. The rate constants for helix formation are about 2 × 106 M?1 s?1 and decrease with increasing temperature yielding an activation enthalpy of ?6 . The rate constants for helix dissociation range from 3 to 250 s?1 and increase with increasing temperature yielding an activation enthalpy of +45 . The kinetic data reported here for 1 M NaCl is compared with previously published results obtained at lower salt concentrations. These data are discussed in terms of the quantitative effect of ionic strength on the kinetics of helix-coil transitions in oligo- and polynucleotides. 相似文献