全文获取类型
收费全文 | 2385篇 |
免费 | 111篇 |
出版年
2021年 | 13篇 |
2020年 | 15篇 |
2019年 | 27篇 |
2018年 | 30篇 |
2017年 | 22篇 |
2016年 | 38篇 |
2015年 | 56篇 |
2014年 | 59篇 |
2013年 | 250篇 |
2012年 | 156篇 |
2011年 | 156篇 |
2010年 | 82篇 |
2009年 | 106篇 |
2008年 | 148篇 |
2007年 | 129篇 |
2006年 | 135篇 |
2005年 | 130篇 |
2004年 | 146篇 |
2003年 | 142篇 |
2002年 | 134篇 |
2001年 | 22篇 |
2000年 | 26篇 |
1999年 | 27篇 |
1998年 | 29篇 |
1997年 | 33篇 |
1996年 | 27篇 |
1995年 | 32篇 |
1994年 | 30篇 |
1993年 | 26篇 |
1992年 | 24篇 |
1991年 | 13篇 |
1990年 | 8篇 |
1989年 | 17篇 |
1988年 | 16篇 |
1987年 | 12篇 |
1986年 | 6篇 |
1985年 | 8篇 |
1984年 | 16篇 |
1983年 | 6篇 |
1982年 | 11篇 |
1981年 | 23篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1978年 | 6篇 |
1977年 | 12篇 |
1976年 | 9篇 |
1975年 | 6篇 |
1974年 | 7篇 |
1973年 | 10篇 |
1966年 | 5篇 |
排序方式: 共有2496条查询结果,搜索用时 15 毫秒
991.
应用FPLC、HPLC系统配合MALDI TOF MS等技术 ,分离得到一个以灭活的Escherichiacoli诱导产生的具有明显杀菌活性的柞蚕抗菌肽CA1。自动蛋白质序列分析仪测定其一级结构为WNPFKELERAGSRVRDAIISAGVAVATVAQATAILK ,含有 36个氨基酸残基 ,经联机检索 ,与cecropinD有 88%的同源性 ,仅有 4个氨基酸残基的差异 ,其中铰链区第 2 1~ 2 3位氨基酸为AGV ,与已知柞蚕抗菌肽A、D铰链区AGP有所不同 ,提示抗菌肽存在多态性。 相似文献
992.
Yoshihiro Yoshiyama Gakuzo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(12):2439-2441
We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores. 相似文献
993.
994.
Hensik Koh Zenzaburo Kumazawa Minoru Nakajima 《Bioscience, biotechnology, and biochemistry》2013,77(6):594-596
Amides, IVa and IVb, which were derived from Grayanotoxin-II reacted very easily under a mild acidic condition to give a product, the structure of which has been established to be Va. 相似文献
995.
996.
997.
998.
Thechromophore in the luminous squid, Symplectoteuthis oualaniensis, was identified to be dehydrocoelenterazine. This chromophore exists as conjugate adduct in the photoprotein. 相似文献
999.
Leukocyte oxidative function was investigated in a more physiological milieu than currently used in the chemiluminescence (CL) technique. Heparinized blood was mixed with 6% dextran-T70 (9:1) and the leukocyte-rich plasma obtained without centrifugation was used for the CL experiments (phagocyte count was adjusted to 0.7 × 106/mL with Hanks' buffer) (method A). In this medium, phagocytes responded to stimulation by opsonized zymosan, producing strong luminescence in the presence of 0.5 m? mol/L MCLA. CL was inhibited by superoxide dismutase, suggesting that the luminescence reaction was attributable to O. Granulocytes were also prepared by the usual method involving centrifugation and were then suspended in plasma (method B). Oxidative function of phagocytes prepared by the two methods was studied together with whole blood as aliquots diluted with Hanks' buffer up to a factor of 1000. Luminescence reached a peak value at a dilution factor of 16, but at very high dilutions luminescence decreased sharply. Significantly higher luminescence values were obtained with samples from method A. Luminescence of whole blood peaked at a dilution factor of 248 but it was less than the value obtained using samples prepared by method A or B. As samples prepared by method A contain all the leukocyte populations, platelets, residual red cells and plasma proteins, the assay of leukocyte-generated reactive oxygens using CL is attained in more physiological conditions than method B in which leukocytes may be damaged owing to repeated centrifugation and hypotonic shock. 相似文献
1000.
Shinichi Kudo Masaaki Onda Ann Rearden Minoru Fukuda 《Journal of medical primatology》1993,22(1):7-12
Glycophorin A, B, and E genes were derived from a common ancestral gene and this gene family appeared during primate evolution, probably between orangutan and gorilla divergences. Based on the study of genomic structures of these human glycophorins and the genetic and immunological study of primate glycophorins, we hypothesize that chimpanzee and gorilla glycophorin B could possess a longer extracellular region and carry a stronger N blood group antigenicity compared with that of the human. 相似文献