全文获取类型
收费全文 | 347篇 |
免费 | 44篇 |
出版年
2021年 | 6篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 6篇 |
2017年 | 4篇 |
2016年 | 8篇 |
2015年 | 14篇 |
2014年 | 21篇 |
2013年 | 14篇 |
2012年 | 23篇 |
2011年 | 18篇 |
2010年 | 13篇 |
2009年 | 17篇 |
2008年 | 19篇 |
2007年 | 18篇 |
2006年 | 14篇 |
2005年 | 14篇 |
2004年 | 11篇 |
2003年 | 10篇 |
2002年 | 10篇 |
2001年 | 8篇 |
2000年 | 15篇 |
1999年 | 6篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 10篇 |
1990年 | 10篇 |
1989年 | 9篇 |
1987年 | 4篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1974年 | 1篇 |
1973年 | 7篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1969年 | 2篇 |
1959年 | 2篇 |
1940年 | 2篇 |
1904年 | 1篇 |
排序方式: 共有391条查询结果,搜索用时 15 毫秒
51.
Costanzo MJ Yabut SC Zhang HC White KB de Garavilla L Wang Y Minor LK Tounge BA Barnakov AN Lewandowski F Milligan C Spurlino JC Abraham WM Boswell-Smith V Page CP Maryanoff BE 《Bioorganic & medicinal chemistry letters》2008,18(6):2114-2121
We have explored a series of spirocyclic piperidine amide derivatives (5) as tryptase inhibitors. Thus, 4 (JNJ-27390467) was identified as a potent, selective tryptase inhibitor with oral efficacy in two animal models of airway inflammation (sheep and guinea pig asthma models). An X-ray co-crystal structure of 4 x tryptase revealed a hydrophobic pocket in the enzyme's active site, which is induced by the phenylethynyl group and is comprised of amino acid residues from two different monomers of the tetrameric protein. 相似文献
52.
Blooms of the brown tide pelagophyte, Aureococcus anophagefferens, have been reported in coastal bays along the east coast of the USA for nearly two decades. Blooms appear to be constrained to shallow bays that have low flushing rates, little riverine input and high salinities (e.g., >28). Nutrient enrichment and coastal eutrophication has been most frequently implicated as the cause of A. anophagefferens and other blooms in coastal bays. We compare N and C dynamics during two brown tide blooms, one in Quantuck Bay, on Long Island, NY in 2000, and the other in Chincoteague Bay, at Public Landing, MD in 2002, with a physically similar site in Chincoteague Bay that did not experience a bloom. We found that the primary forms of nitrogen (N) taken up during the bloom in Quantuck Bay were ammonium and dissolved free amino acids (DFAA) while the primary form of N fueling production at both sites in Chincoteague Bay was urea. At both Chincoteague sites, amino acid carbon (C) was taken up while urea C was not. Even though A. anophagefferens has the ability to take up organic C, during the bloom at Chincoteague Bay, photosynthetic uptake of bicarbonate was the dominant pathway of C acquisition by the >1.2 μm size fraction during the day. C uptake by cells <5.0 μm was insufficient to meet cellular C demand based on the measured N uptake rates and the C:N ratio of particulate material. While cells >1.2 μm did not take up much organic C during the day, smaller cells (>0.2 μm) did. Peptide hydrolysis appeared to play an important role in mobilizing organic matter in Quantuck Bay, where amino acids contributed substantially to N and C uptake, but not in Chincoteague Bay. Dissolved organic N (DON), dissolved organic C (DOC) concentrations and the DOC/DON ratio were higher and total dissolved inorganic N (DIN) concentrations were lower at the bloom site in Chincoteague Bay than at the nonbloom site in the same bay. We conclude that A. anophagefferens is capable of using a wide variety of N and C compounds, and that nutrient inputs, biotic interactions and the dominant recycling pathways determine which compounds are available and which metabolic pathways are active at a particular site. 相似文献
53.
54.
55.
The isoinhibitors of chymotrypsin/elastase from Ascaris lumbricoides: the reactive site 总被引:1,自引:0,他引:1
R J Peanasky Y Bentz G A Homandberg S T Minor D R Babin 《Archives of biochemistry and biophysics》1984,232(1):135-142
Five isoinhibitors of chymotrypsin/elastase present in aqueous extracts of Ascaris were isolated. The reactive site in each isoinhibitor, the peptide bond that during encounter is positioned over the catalytic site in chymotrypsin, is Leu-Met. This bond was hydrolyzed by incubating intact isoinhibitors with 5-25 mol% chymotrypsin at pH 3.2 for 4-6 days (isoinhibitor 1) or 2.5-5 weeks (isoinhibitors 2-5). The reaction under these conditions did not proceed beyond 60% modified isoinhibitor (peptide bond hydrolyzed) and 40% intact inhibitor. The Leu-Met bond, hydrolyzed in modified isoinhibitor, can be resynthesized at pH 7.6 by incubating modified inhibitor with a stoichiometric amount of chymotrypsin bound to Sepharose CL-4B and then dissociating the complex in a kinetically controlled fashion with 5% trichloroacetic acid. The product, intact inhibitor, was obtained in greater than 80% yield. The site in the isoinhibitor that is positioned over the catalytic site in elastase during encounter is the same as for encounter with chymotrypsin. The Leu-Met bond hydrolyzed during encounter with elastase can be resynthesized by chymotrypsin. Chymotrypsin and elastase bind to the inhibitor at the same site. 相似文献
56.
57.
58.
59.
60.
Up to approximately 50 mug of staphylococcal enterotoxin A per plate was produced by cellophane-over-agar cultures. Enterotoxin was determined by a latex agglutination technique. 相似文献