全文获取类型
收费全文 | 614篇 |
免费 | 57篇 |
国内免费 | 4篇 |
出版年
2023年 | 2篇 |
2022年 | 12篇 |
2021年 | 14篇 |
2020年 | 9篇 |
2019年 | 9篇 |
2018年 | 11篇 |
2017年 | 7篇 |
2016年 | 20篇 |
2015年 | 30篇 |
2014年 | 33篇 |
2013年 | 29篇 |
2012年 | 52篇 |
2011年 | 43篇 |
2010年 | 34篇 |
2009年 | 34篇 |
2008年 | 36篇 |
2007年 | 40篇 |
2006年 | 20篇 |
2005年 | 35篇 |
2004年 | 25篇 |
2003年 | 19篇 |
2002年 | 30篇 |
2001年 | 18篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 4篇 |
1997年 | 8篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1989年 | 4篇 |
1988年 | 8篇 |
1986年 | 2篇 |
1985年 | 6篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 5篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1935年 | 1篇 |
1930年 | 2篇 |
1929年 | 1篇 |
排序方式: 共有675条查询结果,搜索用时 15 毫秒
11.
Biological properties of a type C virus isolated from a human X mouse hybrid cell line. 总被引:1,自引:0,他引:1
A F Gazdar E K Russell J Minna 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1975,149(3):688-692
The biological properties of the HMV-1 virus, spontaneously released from a human X C57BL/6 mouse hybrid cell line, were similar to those of RadLV, the prototype B-tropic virus of C57BL/6 mice. Both viruses replicated on B-type mouse cells and in the wild mouse cell line SC-1. The plaque-forming abilities of the two viruses were relatively low, but gradually increased after passage in new host cells. Both viruses were neutralized by AKR antisera but not by FMR antisera. HMV-1 virus could rescue the defective sarcoma genome from S+H- mouse cells. The pseudotype sarcoma virus so produced was deficient in "helper virus" activity. Newborn mice inoculated with HMV-1 virus remained tumor-free over a 1-yr observation period. 相似文献
12.
The evergreen dwarf shrub lingonberry (Vaccinium vitis-idaea L.) was subjected to microtine herbivory simulations to investigate its recovery in a boreal forest. Beside the control (A), the study included three levels of herbivory: clipping of half the annual branches from 50% of ramets (B), removal of 50% of ramets (C), and removal of 100% of ramets (D). Density, growth, and fecundity of the ramets were monitored during the study. New ramets emerged rapidly from dormant basal buds at the base of the removed ramets in groups C and D. After three growth seasons, 60–117% and 42–112% of density and biomass, respectively, had been regained in the groups damaged relative to the control, the difference being insignificant between the treated groups apparently because of the small sample size. Survival, fecundity and net growth of ramets were not affected by the treatment. Mean dry weight of annual branches in new ramets was lower in the damaged groups than the control. The recovery of the lingonberry was unexpectedly good. The results suggest that, since natural herbivory seldom is as heavy as in this work, the time between population density peaks of microtine rodents is usually long enough for the lingonberry to recover in boreal ecosystems. 相似文献
13.
14.
Yang Yiheng Pan Hongli Chen Jie Zhang Zhonghua Liang Minna Feng Xunqiang 《Molecular and cellular biochemistry》2021,476(6):2513-2525
Molecular and Cellular Biochemistry - Multiple circular RNAs (circRNAs) have been identified to act as essential mediators in diverse human cancers. However, the roles of circRNAs in neuroblastoma... 相似文献
15.
Sanna Loppi Paula Korhonen Maria Bouvy‐Liivrand Simone Caligola Tiia A. Turunen Mikko P. Turunen Ana Hernandez de Sande Natalia Koosowska Flavia Scoyni Anna Rosell Teresa García‐Berrocoso Sighild Lemarchant Hiramani Dhungana Joan Montaner Jari Koistinaho Katja M. Kanninen Minna U. Kaikkonen Rosalba Giugno Merja Heinniemi Tarja Malm 《Aging cell》2021,20(1)
16.
Jieru Meng Mourad Majidi Bingliang Fang Lin Ji B. Nebiyou Bekele John D. Minna Jack A. Roth 《PloS one》2013,8(10)
TUSC2-defective gene expression is detected in the majority of lung cancers and is associated with worse overall survival. We analyzed the effects of TUSC2 re-expression on tumor cell sensitivity to the AKT inhibitor, MK2206, and explored their mutual signaling connections, in vitro and in vivo. TUSC2 transient expression in three LKB1-defective non-small cell lung cancer (NSCLC) cell lines combined with MK2206 treatment resulted in increased repression of cell viability and colony formation, and increased apoptotic activity. In contrast, TUSC2 did not affect the response to MK2206 treatment for two LKB1-wild type NSCLC cell lines. In vivo, TUSC2 systemic delivery, by nanoparticle gene transfer, combined with MK2206 treatment markedly inhibited growth of tumors in a human LKB1-defective H322 lung cancer xenograft mouse model. Biochemical analysis showed that TUSC2 transient expression in LKB1-defective NSCLC cells significantly stimulated AMP-activated protein kinase (AMPK) phosphorylation and enzymatic activity. More importantly, AMPK gene knockdown abrogated TUSC2-MK2206 cooperation, as evidenced by reduced sensitivity to the combined treatment. Together, TUSC2 re-expression and MK2206 treatment was more effective in inhibiting the phosphorylation and kinase activities of AKT and mTOR proteins than either single agent alone. In conclusion, these findings support the hypothesis that TUSC2 expression status is a biological variable that potentiates MK2206 sensitivity in LKB1-defective NSCLC cells, and identifies the AMPK/AKT/mTOR signaling axis as an important regulator of this activity. 相似文献
17.
James L. Mulshine Peter Ujhazy Melissa Antman Christine M. Burgess Igor Kuzmin Paul A. Bunn Jr Bruce E. Johnson Jack A. Roth Harvey I. Pass Sheila M. Ross Carolyn R. Aldige Ignacio I. Wistuba John D Minna 《Journal of cellular biochemistry》2020,121(8-9):3986-3999
The intramural the National Cancer Institute (NCI) and more recently the University of Texas Southwestern Medical Center with many different collaborators comprised a complex, multi-disciplinary team that collaborated to generated large, comprehensively annotated, cell-line related research resources which includes associated clinical, and molecular characterization data. This material has been shared in an anonymized fashion to accelerate progress in overcoming lung cancer, the leading cause of cancer death across the world. However, this cell line collection also includes a range of other cancers derived from patient-donated specimens that have been remarkably valuable for other types of cancer and disease research. A comprehensive analysis conducted by the NCI Center for Research Strategy of the 278 cell lines reported in the original Journal of Cellular Biochemistry Supplement, documents that these cell lines and related products have since been used in more than 14 000 grants, and 33 207 published scientific reports. This has resulted in over 1.2 million citations using at least one cell line. Many publications involve the use of more than one cell line, to understand the value of the resource collectively rather than individually; this method has resulted in 2.9 million citations. In addition, these cell lines have been linked to 422 clinical trials and cited by 4700 patents through publications. For lung cancer alone, the cell lines have been used in the research cited in the development of over 70 National Comprehensive Cancer Network clinical guidelines. Finally, it must be underscored again, that patient altruism enabled the availability of this invaluable research resource. 相似文献
18.
19.
Hyun Seok Kim Saurabh Mendiratta Jiyeon Kim Chad Victor Pecot Jill E. Larsen Iryna Zubovych Bo Yeun Seo Jimi Kim Banu Eskiocak Hannah Chung Elizabeth McMillan Sherry Wu Jef De Brabander Kakajan Komurov Jason E. Toombs Shuguang Wei Michael Peyton Noelle Williams Adi F. Gazdar Bruce A. Posner Rolf A. Brekken Anil K. Sood Ralph J. Deberardinis Michael G. Roth John D. Minna Michael A. White 《Cell》2013
20.
Jing Chen Chao Sun Liang Han Xi Lin Li Wang Minna Shen 《Bioscience, biotechnology, and biochemistry》2013,77(8):1246-1256
A full set of optimization procedure was applied to the extraction of anti-viral polysaccharides from Duchesnea indica (Andrews) Focke. By Plackett–Burman factorial design, three parameters (extraction time, extraction temperature, and ratio of water to raw material) were identified as significant to the extraction yield. However, no significant parameters had been identified for antiviral activity. A three-level-three-factor Box–Behnken factorial design was then employed to further optimize the extraction condition. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using appropriate statistical methods. This led to the construction of a response surface indicating the optimal values for each parameter and response studied. Concerning the extraction yield, an extraction at 98.51?ºC for 6.16 h with a ratio of water to raw material of 30.94 mL/g was found to be optimal. Under the optimized conditions, the experimental yield was 6.430 ± 0.078%, which was well matched with the predicted yield of 6.509%. 相似文献