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591.
Many captive stocks of economically or otherwise valuable species were established before the decline of the wild population.
These stocks are potentially valuable sources of genetic variability, but their taxonomic identity and actual value is often
uncertain. We studied the genetics of captive stocks of the threatened lesser white-fronted goose Anser erythropus maintained in Sweden and elsewhere in Europe. Analyses of mtDNA and nuclear microsatellite markers revealed that 36% of the
individuals had a hybrid ancestry. Because the parental species are closely related it is unlikely that our analyses detected
all hybrid individuals in the material. Because no ancestral polymorphism or introgression was observed in samples of wild
populations, it is likely that the observed hybridisation has occurred in captivity. As a consequence of founder effect, drift
and hybridisation, captive stocks were genetically differentiated from the wild populations of the lesser white-fronted goose.
The high level of genetic diversity in the captive stocks is explained at least partially by hybridisation. The present captive
stocks of the lesser white-fronted goose are considered unsuitable for further reintroduction, or supplementation: hybridisation
has involved three species, the number of hybrids is high, and all the investigated captive stocks are similarly affected.
The results highlight the potential shortcomings of using captive-bred individuals in supplementation and reintroduction projects,
when the captive stocks have not been pedigreed and bred according to conservation principles.
Deceased 20 March 2004. 相似文献
592.
593.
We have developed three types of experimental systems for the study of SCCL: (1) serially heterotransplanted tumors in athymic nude mice; (2) continuous, clonable cell cultures; and (3) direct clonogenic assays for tumor specimens. These systems have their own individual advantages, applications, and limitations, but these are interrelated and complementary. The study of these systems has greatly aided our understanding of the biology of SCCL, and its relationship to other lung cancers and the APUD cell system. In addition, new markers for SCCL have been identified, such as a creatine kinase and its BB isoenzyme (CK-BB). These cellular markers may have clinical applications, as serum levels of CK-BB are an indicator of tumor burden. Assays for clonogenic tumor cells may permit selection of optimal drug combinations for the treatment of individual tumors. Variant cultures having the morphology of SCCL, but lacking some or all of the other features, have been identified. While our systems have been used primarily for biological studies, they have clinical applications for both diagnostic and therapeutic purposes. 相似文献
594.
Nerea Abrego Brittni Crosier Panu Somervuo Natalia Ivanova Arusyak Abrahamyan Amir Abdi Karoliina Hmlinen Kaisa Junninen Minna Maunula Jenna Purhonen Otso Ovaskainen 《The ISME journal》2020,14(11):2806
Increasing evidence suggests that degradation of biodiversity in human populated areas is a threat for the ecosystem processes that are relevant for human well-being. Fungi are a megadiverse kingdom that plays a key role in ecosystem processes and affects human well-being. How urbanization influences fungi has remained poorly understood, partially due to the methodological difficulties in comprehensively surveying fungi. Here we show that both aerial and soil fungal communities are greatly poorer in urban than in natural areas. Strikingly, a fivefold reduction in fungal DNA abundance took place in both air and soil samples already at 1 km scale when crossing the edge from natural to urban habitats. Furthermore, in the air, fungal diversity decreased with urbanization even more than in the soil. This result is counterintuitive as fungal spores are known to disperse over large distances. A large proportion of the fungi detectable in the air are specialized to natural habitats, whereas soil fungal communities comprise a large proportion of habitat generalists. The sensitivity of the aerial fungal community to anthropogenic disturbance makes this method a reliable and efficient bioindicator of ecosystem health in urban areas.Subject terms: Community ecology, Fungal ecology 相似文献
595.
596.
597.
Pipsa Hirva Matti Haukka Minna Jakonen Tapani A. Pakkanen 《Inorganica chimica acta》2006,359(3):853-862
Formation of the linear chain ruthenium and osmium carbonyls by successive linkage of mononuclear [M(CO)4Cl2] units and by opening trinuclear clusters [M3(CO)12] and [FeM2(CO)12] (M = Ru, Os) with chlorine gas have been studied by computational DFT methods. Energetically the formation of dinuclear [M2(CO)8Cl2] from [M(CO)4Cl2] units is the most demanding step. The following chain growth by adding new mononuclear units proceeds more easily with nearly constant energy per step. Cluster opening by chlorine gas to obtain trinuclear [M3(CO)12Cl2] is a facile reaction for both ruthenium and osmium clusters as well as for mixed metal clusters. Mixed metal clusters [FeOs2(CO)12] and [FeRu2(CO)12] open preferably between iron-osmium or iron-ruthenium bonds producing linear trinuclear Fe-M-M-type of compound. In the case of mixed metal Os-Ru clusters, the cleavage of Os-Ru bond is not clearly preferred. Fragmentation of the cluster to shorter units cis(Cl)-[M(CO)4Cl2] or [M2(CO)8Cl2] with equatorial chlorides is highly favorable and competes with the cluster opening. No preferences on the bond type (Os-Ru, Os-Os, or Ru-Ru) that are broken can be found in the case of mixed metal Os-Ru clusters. 相似文献
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599.
Anne Toivanen Eveliina Ihanus Minna Mattila Hans U. Lutz Carl G. Gahmberg 《Biochimica et Biophysica Acta (BBA)/General Subjects》2008
Several blood groups, including the LW-blood group were discovered in the first part of last century, but their biochemical characteristics and cellular functions have only more recently been elucidated. The LW-blood group, renamed ICAM-4 (CD242), is red cell specific and belongs to the intercellular adhesion molecule family. ICAM-4 binds to several integrin receptors on blood and endothelial cells and is thus able to form large cellular complexes containing red cells. Its physiological function(s) has remained incompletely understood, but recent work shows that macrophage integrins can bind red cells through this ligand. In this article we discuss molecular properties of major blood group antigens, describe ICAM-4 in more detail, and show that phagocytosis of senescent red cells is in part ICAM-4/β2-integrin dependent. 相似文献
600.
Minna Varikmaa Rita Guzun Alexei Grichine Marcela Gonzalez-Granillo Yves Usson François Boucher Tuuli Kaambre Valdur Saks 《Journal of bioenergetics and biomembranes》2013,45(4):319-331
Mitochondria are dynamic structures for which fusion and fission are well characterized for rapidly dividing cells in culture. Based on these data, it has recently been proposed that high respiratory activity is the result of fusion and formation of mitochondrial reticulum, while fission results in fragmented mitochondria with low respiratory activity. In this work we test the validity of this new hypothesis by analyzing our own experimental data obtained in studies of isolated heart mitochondria, permeabilized cells of cardiac phenotype with different mitochondrial arrangement and dynamics. Additionally, we reviewed published data including electron tomographic investigation of mitochondrial membrane-associated structures in heart cells. Oxygraphic studies show that maximal ADP-dependent respiration rates are equally high both in isolated heart mitochondria and in permeabilized cardiomyocytes. On the contrary, these rates are three times lower in NB HL-1 cells with fused mitochondrial reticulum. Confocal and electron tomographic studies show that there is no mitochondrial reticulum in cardiac cells, known to contain 5,000–10,000 individual, single mitochondria, which are regularly arranged at the level of sarcomeres and are at Z-lines separated from each other by membrane structures, including the T-tubular system in close connection to the sarcoplasmic reticulum. The new structural data in the literature show a principal role for the elaborated T-tubular system in organization of cell metabolism by supplying calcium, oxygen and substrates from the extracellular medium into local domains of the cardiac cells for calcium cycling within Calcium Release Units, associated with respiration and its regulation in Intracellular Energetic Units. 相似文献