蓝舌病毒HbC株与不同种系细胞相互作用及群特异性抗原特征

BTV-HbC株和蓝舌病毒标准株BTV-10分别接种在不同种系细胞如猴肾传代细胞(Vero)、人宫颈癌细胞(Hela)和小鼠神经胶质瘤细胞(C6)等细胞株上,比较研究了BTV-HbC在不同种系细胞上的增殖特征,BTV-HbC与BTV-10在相同细胞上的复制增殖特征,病毒与细胞相互作用的显微和超微结构特征.用免疫交叉反应研究了BTV-HbC株与BTV-10型标准株之间的血清学关系.本研究结合本室对BTV-HbC株基因组图谱分析和蓝舌病毒群特异性抗原编码基因S7的RT-PCR分析,进一步证实了BTV-HbC株可能是一个新的血清型蓝舌病毒.     

巴西酋龙的颌骨

巴山酋龙（Ｄａｔｏｕｓａｕｒｕｓ ｂａｓｈａｎｅｎｓｉｓ Ｄｏｎｇ ａｎｄ Ｔａｎｇ,１９８４）是四川盆地大山铺中侏罗侏罗世蜥脚类恐龙,亦称大头龙。其主要特征之,为硕壮的头骨,文中对保存于重庆自然博物馆的酋龙上下颌骨新材料进行较详细的记述。保存的前上颌骨上,高（前端）、厚分别为１２０ｍｍ,１２５ｍｍ和４０ｍｍ;上颌骨上为２８０ｍｍ;下颌齿骨高为１４５ｍｍ。估计每侧上、下颌齿数分别为１４和１２枚。牙     

人类周期性活动对物种多样性的影响及其预测

人类活动通过作用于栖息地而影响着物种的种群动态,从而影响着物种多样性的变化。首次提出了不同时间尺度人类周期性活动干扰下的多物种竞争动力模式,模拟了千年时间尺度,物种多样性对人类周期性活动的响应过程,开展了人类周期性活动所导致的物种多样性的量的变化的预测研究。有关模拟和预测结果表明:在人类周期性活动的作用下,物种多度变化对栖息地变化的响应也做准周期振荡;同时人类活动强度越大,物种多度振荡的幅度也越大;并且在栖息地减少过程中,人类周期性活动对物种多样性的影响幅度要小于栖息地扩充过程中的。在一个完整的周期内,并没有物种的灭绝,只是物种的多度和强弱关系变化很大。当人类周期性活动仅持续1/4个周期时,最强的几个物种将灭绝,而其它物种做准周期振荡,但振幅相对较小。     

Paradoxical role of β8 integrin on angiogenesis and vasculogenic mimicry in glioblastoma

Glioblastoma multiforme (GBM) is the most aggressive and highly vascularized brain tumor with poor prognosis. Endothelial cell-dependent angiogenesis and tumor cell-dependent Vasculogenic mimicry (VM) synergistically contribute to glioma vascularization and progression. However, the mechanism underlying GBM vascularization remains unclear. In this study, GBM stem cells (GSCs) were divided into high and low β8 integrin (ITGB8) subpopulations. Co-culture assays followed by Cell Counting Kit-8 (CCK-8), migration, Matrigel tube formation, and sprouting assays were conducted to assess the proliferative, migratory and angiogenic capacity of GBM cells and human brain microvascular endothelial cells (hBMECs). An intracranial glioma model was constructed to assess the effect of ITGB8 on tumor vascularization in vivo. Our results indicated that ITGB8 expression was elevated in GSCs and positively associated with stem cell markers in glioma tissues, and could be induced by hypoxia and p38 activation. ITGB8 in GSCs inhibited the angiogenesis of hBMECs in vitro, while it promoted the ability of network formation and expression of VM-related proteins. The orthotopic GBM model showed that ITGB8 contributed to decreased angiogenesis, meanwhile enhanced invasiveness and VM formation. Mechanistic studies indicated that ITGB8-TGFβ1 axis modulates VM and epithelial-mesenchymal transition (EMT) process via Smad2/3-RhoA signaling. Together, our findings demonstrated a differential role for ITGB8 in the regulation of angiogenesis and VM formation in GBM, and suggest that pharmacological inhibition of ITGB8 may represent a promising therapeutic strategy for treatment of GBM.Subject terms: Cancer stem cells, CNS cancer     

Genome-wide CRISPR screen identifies synthetic lethality between DOCK1 inhibition and metformin in liver cancer

Metformin is currently a strong candidate anti-tumor agent in multiple cancers. However, its anti-tumor effectiveness varies among different cancers or subpopulations, potentially due to tumor heterogeneity. It thus remains unclear which hepatocellular carcinoma (HCC) patient subpopulation(s) can benefit from metformin treatment. Here, through a genome-wide CRISPR-Cas9-based knockout screen, we find that DOCK1 levels determine the anti-tumor effects of metformin and that DOCK1 is a synthetic lethal target of metformin in HCC. Mechanistically, metformin promotes DOCK1 phosphorylation, which activates RAC1 to facilitate cell survival, leading to metformin resistance. The DOCK1-selective inhibitor, TBOPP, potentiates anti-tumor activity by metformin in vitro in liver cancer cell lines and patient-derived HCC organoids, and in vivo in xenografted liver cancer cells and immunocompetent mouse liver cancer models. Notably, metformin improves overall survival of HCC patients with low DOCK1 levels but not among patients with high DOCK1 expression. This study shows that metformin effectiveness depends on DOCK1 levels and that combining metformin with DOCK1 inhibition may provide a promising personalized therapeutic strategy for metformin-resistant HCC patients.Supplementary InformationThe online version contains supplementary material available at 10.1007/s13238-022-00906-6.     

界面发酵红曲霉的图像解析*

利用计算机视觉技术研究了红曲霉界面发酵过程中菌体形态变化与菌体生长的关系。在界面培养中通过检测前期菌落面积,后期隆起部分的表征体积——基于颜色变化的生长点分布,可以有效表示菌体生长状况。基于此建立了含有相应形态参数的动力学模型,该模型与常规动力学模型具有相似的表达形式。     

miR-34c inhibits proliferation of glioma by targeting PTP1B

Glioma is one of the most pervasive and invasive primary malignancies in the central nervous sys-tem.Due to its abnormal proliferation,glioma remains hard to cu...     

大草蛉对草地贪夜蛾捕食潜能研究

草地贪夜蛾Spodoptera frugiperda JE Smith于2018年底至2019年初入侵我国后,迅速扩散,并在半年内对西南地区的玉米生产造成为害。田间调查和观测研究表明,田间虽然可见该害虫的天敌,但由于天敌种群数量低,无法形成有效控害的模式。为研究大草蛉对草地贪夜蛾的控害潜能,本研究进行了捕食功能反应试验。结果表明,大草蛉成虫对草地贪夜蛾卵以及大草蛉3龄幼虫对草地贪夜蛾1龄幼虫的捕食效应均能够很好的拟合HollingⅡ功能反应模型。其中,大草蛉成虫对贪夜蛾卵的理论日最大捕食量、瞬时攻击率和处理时间分别为1115.56头、1.004和0.0009 d。大草蛉幼虫对贪夜蛾低龄幼虫的理论日最大捕食量、瞬时攻击率和处理时间分别为358头、1.074和0.003 d。结果表明大草蛉具备对草地贪夜蛾卵和低龄幼虫进行有效控害的潜力,为应用天敌昆虫防治草地贪夜蛾提供了理论依据。