小泡巨鼠(Edwards'rat)一新亚种——海南小泡巨鼠

海南岛中南部山地林区产的小泡巨鼠(Rattus edwardsi)与分布于华南地区的模式亚种(R.e.edwardsi)、分布于四川的亚种(R.e.gigas)明显不同,与国外产的马来半岛亚种(R.e.ciliatus)、苏门答腊亚种(R.e.setiger)及苏门答腊西部亚种(R.e.siporanus)亦有明显的差别,经研究为一新亚种,定名为小泡巨鼠海南亚种Rattus edwardsi hainanensis Xu et Yu subsp.nov.。     

Energy Transfer from Cyanobacterial Phycobilisomes to Spinach Photosystem Ⅱ Particles

The phycobilisomes (PBS) from the thermophilic cyanobacterium Myxosarcina concinna Printz and the PS Ⅱ particles from Spinacia oleracea Mill. were isolated. Their spectral characteristics were defined and the light energy transfer from PBS to PS Ⅱ was studied. As the ion intensity (KH2PO4) of the buffer system increased from 0.1 mol/L to 0.5 mol/L, the efficiency of the energy transfer also increased. The PS Ⅱ particles had a higher water splitting activity. When excited with light at 558 nm, there was a significant rise of the activity of O2 evolution which further indicated that the excitation energy harvested by PBS could transfer to the PS Ⅱ particle from spinach.     

Ultracytochemical Localization of Calcium in Micropyle and Embryo Sac of Brassica napus Before and After Pollination

Potassiam antimonate was used to localize Ca2+ in the micropyle and embryo sac of Brassica napus L. before and after pollination. To identify the nature of the pyroantimonate deposits, energy-dispersive X-ray microanalysis (EDXA) was employed and the deposits were proved to contain calcium pyroantimonate. Image processing system was employed to measure the volume density and the diameter of the deposits. Before and after pollination, calcium was more abundant in the exostome and endostome as compared with the other regions of the integuments, and was concentrated at the apoplast system, i.e. the intercellular matrix of the micropyle canal and the cell wall. Before pollination, each of the two sister synergids accumulated more calcium than the other embryo sac cells. Although the mean diameter of the deposits in the synergid was only two-thirds as that in the egg cell and central cell, the volume density of the deposits in the synergid was about 2.5 times and 1.9 times as that in the egg cell and the central cell respectively. The filiform apparatus and the nucleus had the most abundant calcium within a synergid. After pollination both sister synergids degenerated conspicuously and were characterized by much more deposited calcium (about 2.4 times more than before); and the diameter of the deposits decreased dramatically, which was less than one-third as before. The relationship between calcium distribution and synergid degeneration as well as its functions was discussed.     

里氏木霉纤维二糖水解酶Ⅱ在毕赤酵母中的高效表达

本工作采用巴氏毕赤酵母Pichiapastoris表达系统进行了里氏木霉Trichodermareesei纤维二糖水解酶Ⅱ(CellobiohydrolaseII)的表达。用RT-PCR的方法从经稻草粉诱导的里氏木霉培养物中分离出纤维二糖水解酶Ⅱ的基因,将其插入到巴氏毕赤酵母的表达载体pPICZαA中,并使之处于α-因子信号肽序列的下游,得到重组质粒pPICZαA-cbh2。通过电穿孔的方法用线性化的pPICZαA-cbh2转化巴氏毕赤酵母GS115菌株,经过大量筛选后得到可以高效表达纤维二糖水解酶的毕赤酵母菌株P.pastorisCBHⅡ1。在甲醇诱导的条件下培养P.pastorisCBHⅡ1,培养液中的CMC活性可达到3.82U/mL,SDS-PAGE分析结果表明纤维二糖水解酶在P.pastorisCBHⅡ1中的表达量远远高于里氏木霉。对表达产物进行了LC-MS分析,结果表明所表达的蛋白为里氏木霉的纤维二糖水解酶。     

二杈狗牙花的化学成分

从二杈狗牙花（Ｅｒｖａｔａｍｉａｄｉｖａｒｉｃａｔａ（Ｌ．）Ｂｕｒｋ）中共分离出８个已知化合物,分别为３个环菠萝蜜烷型三萜：ｃｙｃｌｏａｒｔ－２３－ｅｎｅ－３β,２５－ｄｉｏｌ（１）,３β－ｈｙｄｒｏｘｙｃｙｃｌｏａｒｔ－２５－ｅｎｅ－２４－ｏｎｅ（２）,ｃｙｃｌｏｅｕｃａｌｅｎｏｌ（３）;２个吲哚生物碱ｅｒｖａｔａｍｉｎｅ（４）和ｔａｂｅｒｎａｅｍｏｎｔａｎｉｎｅ（５）;其他是β－谷甾醇,３β－乙酰－香树脂醇和胡萝卜甙。它们均由波谱方法鉴定。其中环菠萝蜜烷型三萜系首次被发现存在于狗牙花属植物中     

云南松居群遗传学研究的等位酶分析方法

针对１５个云南松Ｐｉｎｕｓｙｕｎｎａｎｅｎｓｉｓ居群,开展了１４种酶系统的水平切片淀粉凝胶电泳实验,在谱带遗传分析的基础上确定了３３个等位酶位点及其等位基因。其中有３２个等位酶位点是多态的（有２个以上的等位基因）,只有一个单态位点Ｄｉａ－４。有３个等位基因的位点有Ｌａｐ－１、Ｌａｐ－２、Ａａ－３、Ｓｋｄ－１、Ｓｋｄ－２、Ａｄｈ－１、Ａｄｈ－３、Ｇｄｈ、Ｐｇｄ－１、Ｐｇｍ－１、Ｐｇｍ－３、Ｐｇｉ－１、Ｐｇｉ－３、Ｍｄｈ－１、Ｍｅ、Ｇ６ｐｄ、Ｄｉａ－１、Ｔｐｉ－１、Ｔｐｉ－２、Ｔｐｉ－３和Ｔｐｉ－４,有４个等位基因的位点有Ｓｋｄ－３、Ａｄｈ－２、Ｐｇｄ－２、Ｍｄｈ－２、Ｍｄｈ－３、Ｍｄｈ－４和Ｄｉａ－２,有５个等位基因的位点有Ａａｔ－１和Ｄｉａ－３。云南松居群的等位基因平均数Ａ＝２１,在松属中居于中上水平。本研究揭示了云南松居群酶位点及其等位基因带谱的变异式样,为松属植物的遗传多样性研究提供了一批酶位点及其等位基因的参考图谱